PMID- 26417309
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20150929
LR  - 20200930
IS  - 1611-2156 (Print)
IS  - 1611-2156 (Electronic)
IS  - 1611-2156 (Linking)
VI  - 13
DP  - 2014
TI  - Isolation and structure elucidaton of polyphenols from Loranthus micranthus Linn. 
      parasitic on Hevea brasiliensis with antiinflammatory property.
PG  - 859-68
AB  - The present study was carried out to evaluate the anti-inflammatory activities of 
      polyphenols isolated from the leaves of mistletoe (Loranthus micranthus Linn.) 
      parasitic on Hevea brasiliensis. The anti-inflammatory properties of the isolated 
      compounds were evaluated on the basis of their ability to inhibit the production 
      of nitric oxide (NO) and tumuor necrosis factor-alpha (TNF-alpha) in lipopolysaccharide 
      (LPS) activated RAW 264.7 mouse macrophages. Semi-preparative HPLC separation of 
      the ethyl acetate (EtOAc) and butanol (n-BuOH) fractions of the leaves of 
      mistletoe (Loranthus micranthus Linn) parasitic on Hevea brasiliensis led to the 
      isolation of four polyphenols: 3-O-(3,4,5-trimethoxybenzoyl)-(-)-epicatechin 
      (TMECG) (1); (-)-epicatechin-3-O-(3''-O-methyl)-gallate (ECG3''Me) (2); rutin (3) 
      and peltatoside (4). Compounds 1-4 were isolated for the first time from this 
      plant while 1 was isolated for the first time in nature. These compounds (1-4) 
      were readily identified by comparison of their spectroscopic data with those 
      reported in the literature. The polyphenols proved to have anti-inflammatory 
      activity as evidenced by the suppression of inducible nitric oxide (iNO) and 
      cytokine (TNF-alpha) levels in the culture supernatant of lipopolysaccharide 
      (LPS)-stimulated RAW 264.7 murine macrophages. However, the study showed that the 
      quercetin diglycosides showed stronger inhibition of proinflammatory mediators 
      than the epicatechin derivates. These data provide evidence that polyphenolic 
      compounds isolated from the mistletoe parasitic on Hevea brasiliensis may 
      contribute to its anti-inflammatory properties by inhibiting the expression of 
      inducible nitric oxide and proinflammatory cytokines such as tumour necrosis 
      factor-alpha.
FAU - Agbo, Matthias Onyebuchi
AU  - Agbo MO
AD  - Institute of Pharmaceutical Biology and Biotechnology, Heinrich-Heine University, 
      Dusseldorf, Germany ; Department of Pharmacology & Toxicology, University of 
      Nigeria, Nsukka, Enugu State Nigeria.
FAU - Nworu, Chukwuemeka Sylvester
AU  - Nworu CS
AD  - Department of Pharmacology & Toxicology, University of Nigeria, Nsukka, Enugu 
      State Nigeria.
FAU - Okoye, Festus Basden Chied
AU  - Okoye FB
AD  - Department of Pharmaceutical and Medicinal Chemistry, Nnamdi Azikiwe University, 
      Awka, Anambra State, Nigeria.
FAU - Osadebe, Patience Ogoamaka
AU  - Osadebe PO
AD  - Department of Pharmaceutical and Medicinal Chemistry, University of Nigeria, 
      Nsukka, Enugu State, Nigeria.
LA  - eng
PT  - Journal Article
DEP - 20140820
PL  - Germany
TA  - EXCLI J
JT  - EXCLI journal
JID - 101299402
PMC - PMC4464429
OTO - NOTNLM
OT  - RAW 264.7 murine macrophages
OT  - TNF-alpha
OT  - inducible nitric oxide
OT  - inflammation
OT  - mistletoe
OT  - polyphenols
OT  - structural elucidation
OT  - tumour necrosis factor-alpha
EDAT- 2014/01/01 00:00
MHDA- 2014/01/01 00:01
PMCR- 2014/08/20
CRDT- 2015/09/30 06:00
PHST- 2014/04/01 00:00 [received]
PHST- 2014/07/21 00:00 [accepted]
PHST- 2015/09/30 06:00 [entrez]
PHST- 2014/01/01 00:00 [pubmed]
PHST- 2014/01/01 00:01 [medline]
PHST- 2014/08/20 00:00 [pmc-release]
AID - 2014-282 [pii]
AID - Doc859 [pii]
PST - epublish
SO  - EXCLI J. 2014 Aug 20;13:859-68. eCollection 2014.