PMID- 26417427
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230411
IS  - 2041-6520 (Print)
IS  - 2041-6539 (Electronic)
IS  - 2041-6520 (Linking)
VI  - 6
IP  - 7
DP  - 2015 Jul 1
TI  - Significant improvement of oxidase activity through the genetic incorporation of 
      a redox-active unnatural amino acid.
PG  - 3881-3885
AB  - While nature employs various covalent and non-covalent strategies to modulate 
      tyrosine (Y) redox potential and pK (a) in order to optimize enzyme activities, 
      such approaches have not been systematically applied for the design of functional 
      metalloproteins. Through the genetic incorporation of 3-methoxytyrosine (OMeY) 
      into myoglobin, we replicated important features of cytochrome c oxidase (CcO) in 
      this small soluble protein, which exhibits selective O(2) reduction activity 
      while generating a small amount of reactive oxygen species (ROS). These results 
      demonstrate that the electron donating ability of a tyrosine residue in the 
      active site is important for CcO function. Moreover, we elucidated the structural 
      basis for the genetic incorporation of OMeY into proteins by solving the X-ray 
      structure of OMeY specific aminoacyl-tRNA synthetase complexed with OMeY.
CI  - This journal is (c) The Royal Society of Chemistry 2015.
FAU - Yu, Yang
AU  - Yu Y
AD  - Center of Biophysics and Computational Biology and Department of Chemistry , 
      University of Illinois at Urbana-Champaign , Urbana , Illinois 61801 , USA . 
      Email: yi-lu@illinois.edu.
FAU - Zhou, Qing
AU  - Zhou Q
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Wang, Li
AU  - Wang L
AD  - School of Life Sciences , University of Science and Technology of China , Hefei , 
      Anhui 230026 , China.
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Liu, Xiaohong
AU  - Liu X
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Hu, Meirong
AU  - Hu M
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Dong, Jianshu
AU  - Dong J
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Li, Jiasong
AU  - Li J
AD  - School of Life Sciences , University of Science and Technology of China , Hefei , 
      Anhui 230026 , China.
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Lv, Xiaoxuan
AU  - Lv X
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Ouyang, Hanlin
AU  - Ouyang H
AD  - Center of Biophysics and Computational Biology and Department of Chemistry , 
      University of Illinois at Urbana-Champaign , Urbana , Illinois 61801 , USA . 
      Email: yi-lu@illinois.edu.
FAU - Li, Han
AU  - Li H
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Gao, Feng
AU  - Gao F
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Gong, Weimin
AU  - Gong W
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
FAU - Lu, Yi
AU  - Lu Y
AD  - Center of Biophysics and Computational Biology and Department of Chemistry , 
      University of Illinois at Urbana-Champaign , Urbana , Illinois 61801 , USA . 
      Email: yi-lu@illinois.edu.
FAU - Wang, Jiangyun
AU  - Wang J
AD  - Laboratory of RNA Biology , Institute of Biophysics , Chinese Academy of Sciences 
      , 15 Datun Road, Chaoyang District , Beijing , 100101 , China . Email: 
      jwang@ibp.ac.cn.
LA  - eng
GR  - R01 GM062211/GM/NIGMS NIH HHS/United States
PT  - Journal Article
DEP - 20150413
PL  - England
TA  - Chem Sci
JT  - Chemical science
JID - 101545951
PMC - PMC4583198
MID - NIHMS688189
EDAT- 2015/09/30 06:00
MHDA- 2015/09/30 06:01
PMCR- 2015/07/01
CRDT- 2015/09/30 06:00
PHST- 2015/03/29 00:00 [received]
PHST- 2015/04/13 00:00 [accepted]
PHST- 2015/09/30 06:00 [pubmed]
PHST- 2015/09/30 06:01 [medline]
PHST- 2015/09/30 06:00 [entrez]
PHST- 2015/07/01 00:00 [pmc-release]
AID - c5sc01126d [pii]
AID - 10.1039/C5SC01126D [doi]
PST - ppublish
SO  - Chem Sci. 2015 Jul 1;6(7):3881-3885. doi: 10.1039/C5SC01126D. Epub 2015 Apr 13.