PMID- 26418311
OWN - NLM
STAT- MEDLINE
DCOM- 20160531
LR  - 20220316
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 9
DP  - 2015
TI  - Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and 
      Allergen-Induced Inflammation by Targeting GATA-3.
PG  - e0139322
LID - 10.1371/journal.pone.0139322 [doi]
LID - e0139322
AB  - Mast cell (MC) degranulation is the foundation of the acute phase of allergic 
      rhinitis (AR). Previously, downregulation of GATA binding protein 3 (GATA-3) was 
      shown to suppress MC activation in an AR mouse model. Binding of microRNA-135a 
      (miR-135a) to GATA-3 was also observed, and overexpression of this miRNA 
      decreased GATA-3 mRNA and protein expression. However, the effects of miR-135a on 
      MCs during AR are currently unknown. In the present study, we utilized a 
      lentiviral (LV) vector to intranasally administer miR-135a to ovalbumin 
      (OVA)-sensitized AR mice. Following miR-135a treatment, the total serum IgE 
      concentration observed during AR was significantly reduced. In the nasal mucosa, 
      the expression of T-box expressed in T cells (T-bet) was higher, whereas that of 
      GATA-3 was lower in the AR mice following miRNA treatment. Notably, during AR, 
      the ratio of type 1 T-helper cells (Th1) to type 2 (Th2) cells in the spleen is 
      unbalanced, favoring Th2. However, administering miR-135a to the AR mice appeared 
      to balance this ratio by increasing and decreasing the percentage of Th1 and Th2 
      cells, respectively. MiR-135a also appeared to strongly suppress the infiltration 
      of eosinophils and MCs into the nasal mucosa, and it was specifically localized 
      in the MCs, suggesting that its influence is modulated through regulation of 
      GATA-3 in these cells. Additional work identifying the full therapeutic potential 
      of miR-135a in the treatment of AR and diseases involving allergen-induced 
      inflammation is warranted.
FAU - Deng, Yu-Qin
AU  - Deng YQ
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei, China.
FAU - Yang, Ya-Qi
AU  - Yang YQ
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei, China.
FAU - Wang, Shui-Bin
AU  - Wang SB
AD  - Department of Otolaryngology-Head and Neck Surgery, Yichang Yiling Hospital, 
      Yichang, Hubei, China.
FAU - Li, Fen
AU  - Li F
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei, China.
FAU - Liu, Meng-Zhi
AU  - Liu MZ
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei, China.
FAU - Hua, Qing-Quan
AU  - Hua QQ
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei, China.
FAU - Tao, Ze-Zhang
AU  - Tao ZZ
AD  - Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan 
      University, Wuhan, Hubei, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150929
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Allergens)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Gata3 protein, mouse)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn135 microRNA, mouse)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Administration, Intranasal
MH  - Allergens/immunology
MH  - Animals
MH  - Flow Cytometry
MH  - GATA3 Transcription Factor/genetics/*immunology/metabolism
MH  - Gene Expression/immunology
MH  - Genetic Vectors/administration & dosage/genetics
MH  - Lentivirus/genetics
MH  - Mast Cells/*immunology/metabolism
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/*immunology/metabolism
MH  - Microscopy, Confocal
MH  - Nasal Mucosa/immunology/metabolism
MH  - Ovalbumin/immunology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Rhinitis, Allergic/genetics/*immunology
MH  - T-Box Domain Proteins/genetics/immunology/metabolism
MH  - Th1 Cells/immunology/metabolism
MH  - Th2 Cells/immunology/metabolism
PMC - PMC4587974
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/09/30 06:00
MHDA- 2016/06/01 06:00
PMCR- 2015/09/29
CRDT- 2015/09/30 06:00
PHST- 2015/03/20 00:00 [received]
PHST- 2015/09/11 00:00 [accepted]
PHST- 2015/09/30 06:00 [entrez]
PHST- 2015/09/30 06:00 [pubmed]
PHST- 2016/06/01 06:00 [medline]
PHST- 2015/09/29 00:00 [pmc-release]
AID - PONE-D-15-12215 [pii]
AID - 10.1371/journal.pone.0139322 [doi]
PST - epublish
SO  - PLoS One. 2015 Sep 29;10(9):e0139322. doi: 10.1371/journal.pone.0139322. 
      eCollection 2015.