PMID- 26418753
OWN - NLM
STAT- MEDLINE
DCOM- 20161010
LR  - 20161230
IS  - 1552-4965 (Electronic)
IS  - 1549-3296 (Linking)
VI  - 104
IP  - 2
DP  - 2016 Feb
TI  - Biocompatibility of common polyimides with human endothelial cells for a 
      cardiovascular microsensor.
PG  - 406-12
LID - 10.1002/jbm.a.35578 [doi]
AB  - The cardiovasculature is an emerging niche for polyimide microdevices, yet the 
      biocompatibility of polyimides with human endothelial cells has not been reported 
      in the literature. In this study, we have evaluated an experimental 
      polyimide-based pressure sensor for biological safety to determine its 
      suitability for intravascular operation by using an in vitro model of human 
      endothelium, following ISO 10993-5 protocols for extract tests and direct contact 
      tests. First, SV-HCEC cells were incubated with extracts derived from common 
      microfabrication polyimides utilized in the transducer (PMDA-ODA, BPDA-PPD, and a 
      proprietary thermoplastic adhesive), and then labeled with selective probes to 
      evaluate the effect of the polyimides on mitochondria and cell viability. Flow 
      cytometry analysis showed that incubation of SV-HCECs with polyimide extracts 
      resulted in no significant change in mitochondrial membrane potential (detected 
      by JC-1) or apoptotic (annexin V) and necrotic (propidium iodide) cell death, 
      when compared to incubation with extracts of high-density polyethylene (HDPE) and 
      untreated cells used as negative controls. Second, primary human endothelial 
      cells were incubated in direct contact with the completed sensor and then labeled 
      with selective probes for live-dead analysis (calcein-AM, ethidium homodimer-1). 
      Endothelial cells showed no loss of viability when compared to negative controls. 
      Combined, the studies show no significant change in early markers of stress or 
      more strict markers of viability in endothelial cells treated with the polyimides 
      tested. We conclude that these common microfabrication polyimides and the derived 
      sensor are not cytotoxic to human endothelial cells, the primary cell type that 
      cardiovascular sensors will contact in vivo.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Starr, Peter
AU  - Starr P
AD  - University of Texas Health Science Center, San Antonio, Texas, 78229.
AD  - University of Texas, San Antonio, Texas, 78249.
FAU - Agrawal, C Mauli
AU  - Agrawal CM
AD  - University of Texas, San Antonio, Texas, 78249.
FAU - Bailey, Steven
AU  - Bailey S
AD  - University of Texas Health Science Center, San Antonio, Texas, 78229.
AD  - University of Texas, San Antonio, Texas, 78249.
LA  - eng
PT  - Journal Article
DEP - 20151015
PL  - United States
TA  - J Biomed Mater Res A
JT  - Journal of biomedical materials research. Part A
JID - 101234237
RN  - 0 (Resins, Synthetic)
RN  - 39355-34-5 (polyimide resin)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Endothelial Cells/*metabolism/pathology
MH  - Humans
MH  - Materials Testing/*methods
MH  - Mitochondrial Membranes/*drug effects
MH  - Polyethylene/chemistry
MH  - Resins, Synthetic/*chemistry
OTO - NOTNLM
OT  - biocompatibility
OT  - cardiovascular
OT  - microfabrication
OT  - polyimides
OT  - sensor
EDAT- 2015/09/30 06:00
MHDA- 2016/10/11 06:00
CRDT- 2015/09/30 06:00
PHST- 2015/06/01 00:00 [received]
PHST- 2015/08/18 00:00 [revised]
PHST- 2015/09/24 00:00 [accepted]
PHST- 2015/09/30 06:00 [entrez]
PHST- 2015/09/30 06:00 [pubmed]
PHST- 2016/10/11 06:00 [medline]
AID - 10.1002/jbm.a.35578 [doi]
PST - ppublish
SO  - J Biomed Mater Res A. 2016 Feb;104(2):406-12. doi: 10.1002/jbm.a.35578. Epub 2015 
      Oct 15.