PMID- 26424042
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151001
LR  - 20200930
IS  - 2093-0879 (Print)
IS  - 2093-0887 (Electronic)
IS  - 2093-0879 (Linking)
VI  - 21
IP  - 4
DP  - 2015 Oct 1
TI  - Corticotropin-releasing Factor Changes the Phenotype and Function of Dendritic 
      Cells in Mouse Mesenteric Lymph Nodes.
PG  - 571-80
LID - 10.5056/jnm15019 [doi]
AB  - BACKGROUND/AIMS: Dendritic cells (DCs) are a significant contributor to the 
      pathology of numerous chronic inflammatory autoimmune disorders; however, the 
      effects of Corticotropin-releasing factor (CRF) on intestinal DCs are poorly 
      understood. In this study, we investigated the role of CRF in alterations of 
      intestinal dendritic cell phenotype and function. METHODS: Mouse mesenteric lymph 
      node dendritic cells (MLNDCs) were obtained using magnetic bead sorting. Surface 
      expression of CRF receptor type 1 (CRF-R1) and CRF-R2 was determined by 
      double-labeling immunofluorescence and quantitative polymerase chain reaction 
      (qPCR) and MLNDCs were subsequently exposed to CRF in the presence or absence of 
      CRF-R1 and CRF-R2 antagonists. Expression of surface molecules (MHC-I and MHC-II) 
      and co-stimulatory molecules (CD80 and CD86) was determined by flow cytometric 
      and western blot analyses, and the T cell stimulatory capacity of MLNDCs was 
      evaluated by mixed lymphocyte reaction. RESULTS: Immunofluorescent staining and 
      quatitative polymerase chain reaction indicated that both the CRF receptors 
      (CRF-R1 and CRF-2) are expressed on the surface of MLNDCs. Exposure to CRF 
      increased the expression of MHC-II on MLNDCs as well as their capacity to 
      stimulate T cell proliferation. MLNDCs treated with CRF-R1 antagonist exhibited a 
      phenotype characterized by a less activated state and reduced surface expression 
      of MHC-II, and consequently showed reduced capacity to stimulate T cells. In 
      contrast, treatment of MLNDCs with CRF-R2 antagonist yielded an opposite result. 
      CONCLUSIONS: CRF can alter the phenotype and function of intestinal DCs through 
      direct action on CRF-R1 and CRF-R2, and activation of the CRF-R1 and CRF-R2 
      pathways yields opposing outcomes.
FAU - Meng, Li
AU  - Meng L
AUID- ORCID: 0000-0001-8921-2533
AD  - Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou, China.
FAU - Lu, Zhang
AU  - Lu Z
AD  - Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou, China.
FAU - Xiaoteng, Wang
AU  - Xiaoteng W
AD  - Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou, China.
FAU - Yue, Hu
AU  - Yue H
AD  - Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou, China.
FAU - Bin, Lu
AU  - Bin L
AUID- ORCID: 0000-0002-3381-6976
AD  - Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou, China.
FAU - Lina, Meng
AU  - Lina M
AD  - Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou, China.
FAU - Zhe, Chen
AU  - Zhe C
AD  - Department of Gastroenterology, First Affiliated Hospital of Zhejiang Chinese 
      Medical University, Hangzhou, China.
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Neurogastroenterol Motil
JT  - Journal of neurogastroenterology and motility
JID - 101530189
PMC - PMC4622140
OTO - NOTNLM
OT  - Corticotropin-releasing hormone
OT  - Dendritic cells
OT  - Immunity
OT  - cellular
EDAT- 2015/10/02 06:00
MHDA- 2015/10/02 06:01
PMCR- 2015/10/01
CRDT- 2015/10/02 06:00
PHST- 2015/01/30 00:00 [received]
PHST- 2015/06/11 00:00 [revised]
PHST- 2015/06/30 00:00 [accepted]
PHST- 2015/10/02 06:00 [entrez]
PHST- 2015/10/02 06:00 [pubmed]
PHST- 2015/10/02 06:01 [medline]
PHST- 2015/10/01 00:00 [pmc-release]
AID - jnm15019 [pii]
AID - jnm-21-571 [pii]
AID - 10.5056/jnm15019 [doi]
PST - ppublish
SO  - J Neurogastroenterol Motil. 2015 Oct 1;21(4):571-80. doi: 10.5056/jnm15019.