PMID- 26424612
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20201212
IS  - 1573-6849 (Electronic)
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 23
IP  - 4
DP  - 2015 Dec
TI  - Identification of the centromeric repeat in the threespine stickleback fish 
      (Gasterosteus aculeatus).
PG  - 767-79
LID - 10.1007/s10577-015-9495-3 [doi]
AB  - Centromere sequences exist as gaps in many genome assemblies due to their 
      repetitive nature. Here we take an unbiased approach utilizing centromere protein 
      A (CENP-A) chomatin immunoprecipitation followed by high-throughput sequencing to 
      identify the centromeric repeat sequence in the threespine stickleback fish 
      (Gasterosteus aculeatus). A 186-bp, AT-rich repeat was validated as centromeric 
      using both fluorescence in situ hybridization (FISH) and immunofluorescence 
      combined with FISH (IF-FISH) on interphase nuclei and metaphase spreads. This 
      repeat hybridizes strongly to the centromere on all chromosomes, with the 
      exception of weak hybridization to the Y chromosome. Together, our work provides 
      the first validated sequence information for the threespine stickleback 
      centromere.
FAU - Cech, Jennifer N
AU  - Cech JN
AD  - Divisions of Human Biology and Basic Sciences, Fred Hutchinson Cancer Research 
      Center, 1100 Fairview Ave North, Mailstop C2-023, Seattle, WA, 98109, USA.
AD  - Graduate Program in Molecular and Cellular Biology, University of Washington, 
      Seattle, WA, 98195, USA.
FAU - Peichel, Catherine L
AU  - Peichel CL
AD  - Divisions of Human Biology and Basic Sciences, Fred Hutchinson Cancer Research 
      Center, 1100 Fairview Ave North, Mailstop C2-023, Seattle, WA, 98109, USA. 
      cpeichel@fhcrc.org.
LA  - eng
GR  - T32 CA009657/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20150930
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Autoantigens)
RN  - 0 (Centromere Protein A)
RN  - 0 (Chromosomal Proteins, Non-Histone)
SB  - IM
MH  - Animals
MH  - Autoantigens/chemistry/genetics/metabolism
MH  - Base Sequence
MH  - Binding Sites
MH  - Centromere/*genetics
MH  - Centromere Protein A
MH  - Chromosomal Proteins, Non-Histone/chemistry/genetics/metabolism
MH  - Female
MH  - Fishes/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Protein Binding
MH  - Sequence Alignment
MH  - *Tandem Repeat Sequences
MH  - Y Chromosome
PMC - PMC7728544
MID - NIHMS1650517
OTO - NOTNLM
OT  - CENP-A
OT  - Centromere
OT  - ChIP-seq
OT  - Gasterosteus aculeatus
OT  - Threespine stickleback
EDAT- 2015/10/02 06:00
MHDA- 2016/09/16 06:00
PMCR- 2020/12/10
CRDT- 2015/10/02 06:00
PHST- 2015/07/24 00:00 [received]
PHST- 2015/09/17 00:00 [accepted]
PHST- 2015/09/11 00:00 [revised]
PHST- 2015/10/02 06:00 [entrez]
PHST- 2015/10/02 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
PHST- 2020/12/10 00:00 [pmc-release]
AID - 10.1007/s10577-015-9495-3 [pii]
AID - 10.1007/s10577-015-9495-3 [doi]
PST - ppublish
SO  - Chromosome Res. 2015 Dec;23(4):767-79. doi: 10.1007/s10577-015-9495-3. Epub 2015 
      Sep 30.