PMID- 26428233
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20221207
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 100
IP  - 2
DP  - 2016 Jan
TI  - Bioaugmentation of anaerobic sludge digestion with iron-reducing bacteria: 
      process and microbial responses to variations in hydraulic retention time.
PG  - 927-37
LID - 10.1007/s00253-015-7018-y [doi]
AB  - Although anaerobic digestion (AD) is a widely used option to manage waste 
      activated sludge (WAS), there are some drawbacks related to its slow reaction 
      rate and low energy productivity. This study examined an anaerobic WAS digester, 
      augmented with an iron-reducing microbial consortium, relative to changes in 
      microbial community structure and process performance at decreasing hydraulic 
      retention times (HRTs) of 20 to 10 days. The enhanced methanation performance 
      (approximately 40 % increase in methane yield) by the bioaugmentation was 
      sustained until the HRT was decreased to 12.5 days, under Fe(3+)-rich conditions 
      (ferric oxyhydroxide, 20 mM Fe). Enhanced iron-reducing activity was evidenced by 
      the increased Fe(2+) to total Fe ratio maintained above 50 % during the stable 
      operational phases. A further decrease in HRT to 10 days resulted in a 
      significant performance deterioration, along with a drop in the Fe(2+) to total 
      Fe ratio to <35 %, after four turnovers of operation. Prevailing existence of 
      putative iron-reducing bacteria (IRBs) was identified by denaturing gradient gel 
      electrophoresis (DGGE), with Spirochaetaceae- and Thauera-related organisms being 
      dominant members, and clear dominance shifts among them with respect to decrease 
      in HRT were observed. Lowering HRT led to evident shifts in bacterial community 
      structure likely associated with washout of IRBs, leading to decreases in iron 
      respiration activity and AD performance at a lower HRT. The bacterial community 
      structure shifted dynamically over phases, and the community transitions 
      correlated well with the changes in process performance. Overall, the combined 
      biostimulation and bioaugmentation investigated in this study proved effective 
      for enhanced methane recovery from anaerobic WAS digestion, which suggests an 
      interesting potential for high-rate AD.
FAU - Baek, Gahyun
AU  - Baek G
AD  - School of Urban and Environmental Engineering, Ulsan National Institute of 
      Science and Technology (UNIST), 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, 
      689-798, Republic of Korea.
FAU - Kim, Jaai
AU  - Kim J
AD  - School of Urban and Environmental Engineering, Ulsan National Institute of 
      Science and Technology (UNIST), 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, 
      689-798, Republic of Korea.
FAU - Shin, Seung Gu
AU  - Shin SG
AD  - School of Environmental Science and Engineering, POSTECH, Pohang, Gyungbuk, 
      790-784, Republic of Korea.
FAU - Lee, Changsoo
AU  - Lee C
AD  - School of Urban and Environmental Engineering, Ulsan National Institute of 
      Science and Technology (UNIST), 50 UNIST-gil, Eonyang-eup, Ulju-gun, Ulsan, 
      689-798, Republic of Korea. cslee@unist.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151001
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (Sewage)
RN  - 0 (Waste Water)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Anaerobiosis
MH  - Bacteria/*metabolism
MH  - Biological Oxygen Demand Analysis
MH  - *Bioreactors
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Iron/*metabolism
MH  - Microbial Consortia/*physiology
MH  - Sewage/*microbiology
MH  - Spirochaetaceae/metabolism
MH  - Waste Disposal, Fluid
MH  - Wastewater/microbiology
OTO - NOTNLM
OT  - Anaerobic digestion
OT  - Bioaugmentation
OT  - Ferric oxyhydroxide
OT  - Iron-reducing bacteria
OT  - Microbial community structure
EDAT- 2015/10/03 06:00
MHDA- 2016/11/01 06:00
CRDT- 2015/10/03 06:00
PHST- 2015/08/02 00:00 [received]
PHST- 2015/09/17 00:00 [accepted]
PHST- 2015/09/07 00:00 [revised]
PHST- 2015/10/03 06:00 [entrez]
PHST- 2015/10/03 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
AID - 10.1007/s00253-015-7018-y [pii]
AID - 10.1007/s00253-015-7018-y [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2016 Jan;100(2):927-37. doi: 
      10.1007/s00253-015-7018-y. Epub 2015 Oct 1.