PMID- 2643036
OWN - NLM
STAT- MEDLINE
DCOM- 19890223
LR  - 20190820
IS  - 0166-6851 (Print)
IS  - 0166-6851 (Linking)
VI  - 32
IP  - 1
DP  - 1989 Jan 1
TI  - De novo and salvage biosynthesis of pteroylpentaglutamates in the human malaria 
      parasite, Plasmodium falciparum.
PG  - 25-37
AB  - Plasmodium falciparum was shown to synthesize pteroylpolyglutamate de novo from 
      guanosine 5'-triphosphate (GTP), p-aminobenzoate (PABA), and L-glutamate (L-Glu). 
      The parasite also had the capacity to synthesize pteroylpolyglutamate from both 
      intact and degradation moieties (p-aminobenzoylglutamate and pterin-aldehyde) of 
      exogenous folate added into the growth medium. The major product was identified 
      as 5-methyl-tetrahydroteroylpentaglutamate following exposure to 
      pteroylpolyglutamate hydrolase and oxidative degradation of the C9-N10 bond in 
      the molecule and identification of products by reversed-phase high performance 
      liquid chromatography. Inhibition of pteroylpentaglutamate synthesis from the 
      radiolabelled metabolic precursors (GTP, PABA, L-Glu) and folate by the 
      antifolate antimalarials, pyrimethamine and sulfadoxine at therapeutic 
      concentrations, may suggest the existence of a unique biosynthetic pathway in the 
      malaria parasite.
FAU - Krungkrai, J
AU  - Krungkrai J
AD  - Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, 
      Thailand.
FAU - Webster, H K
AU  - Webster HK
FAU - Yuthavong, Y
AU  - Yuthavong Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Netherlands
TA  - Mol Biochem Parasitol
JT  - Molecular and biochemical parasitology
JID - 8006324
RN  - 0 (Pterins)
RN  - 0 (Pteroylpolyglutamic Acids)
RN  - 33611-85-7 (pteroylpentaglutamic acid)
RN  - 39555-46-9 (5-methyltetrahydropteroylpentaglutamate)
RN  - 88463U4SM5 (Sulfadoxine)
RN  - 935E97BOY8 (Folic Acid)
RN  - Z3614QOX8W (Pyrimethamine)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Erythrocytes/microbiology
MH  - Folic Acid/*analogs & derivatives/biosynthesis
MH  - Humans
MH  - Plasmodium falciparum/*metabolism
MH  - Pterins/biosynthesis
MH  - Pteroylpolyglutamic Acids/*biosynthesis
MH  - Pyrimethamine/pharmacology
MH  - Sulfadoxine/pharmacology
EDAT- 1989/01/01 00:00
MHDA- 1989/01/01 00:01
CRDT- 1989/01/01 00:00
PHST- 1989/01/01 00:00 [pubmed]
PHST- 1989/01/01 00:01 [medline]
PHST- 1989/01/01 00:00 [entrez]
AID - 0166-6851(89)90126-6 [pii]
AID - 10.1016/0166-6851(89)90126-6 [doi]
PST - ppublish
SO  - Mol Biochem Parasitol. 1989 Jan 1;32(1):25-37. doi: 10.1016/0166-6851(89)90126-6.