PMID- 26432335
OWN - NLM
STAT- MEDLINE
DCOM- 20170210
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 37
IP  - 3
DP  - 2016 Mar
TI  - Enantiomeric CopA3 dimer peptide suppresses cell viability and tumor xenograft 
      growth of human gastric cancer cells.
PG  - 3237-45
LID - 10.1007/s13277-015-4162-z [doi]
AB  - The CopA3 dimer peptide is a coprisin analog that has an anticancer effect 
      against human cancer cells in vitro. In this study, we investigated the 
      anticancer activity of the enantiomeric CopA3 dimer peptide in human gastric 
      cancer cell lines as well as in an in vivo tumor xenograft model. Enantiomeric 
      CopA3 reduced gastric cancer cell viability and exhibited cytotoxicity against 
      cancer cells. Enantiomeric CopA3-induced cell death was mediated by specific 
      interactions with phosphatidylserine and phosphatidylcholine, membrane components 
      that are enriched in cancer cells, in a calcein leakage assay. Moreover, acridine 
      orange/ethidium bromide staining, flow cytometric analysis, and Western blot 
      analysis showed that enantiomeric CopA3 induced apoptotic and necrotic gastric 
      cancer cell death. The antitumor effect was also observed in a mouse tumor 
      xenograft model in which intratumoral inoculation of the peptide resulted in a 
      significant decrease in the SNU-668 gastric cancer tumor volume. In addition, 
      periodic acid-Schiff and hematoxylin staining and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay revealed apoptotic and 
      necrotic cell death in tumor masses treated with greater than 150 mug CopA3. 
      Collectively, these results indicate that the enantiomeric CopA3 dimer peptide 
      induces apoptosis and necrosis of gastric cancer cells in vitro and in vivo, 
      indicating that the peptide is a potential candidate for the treatment of gastric 
      cancer, which is a common cause of cancer and cancer deaths worldwide.
FAU - Lee, Joon Ha
AU  - Lee JH
AD  - Department of Agricultural Biology, National Academy of Agricultural Science, 
      Rural Development Administration, Wanju, 55365, South Korea.
FAU - Kim, In-Woo
AU  - Kim IW
AD  - Department of Agricultural Biology, National Academy of Agricultural Science, 
      Rural Development Administration, Wanju, 55365, South Korea.
FAU - Shin, Yong Pyo
AU  - Shin YP
AD  - Division of Life Sciences, Hoseo University, Asan, 31499, South Korea.
FAU - Park, Ho Jin
AU  - Park HJ
AD  - Division of Life Sciences, Hoseo University, Asan, 31499, South Korea.
FAU - Lee, Young Shin
AU  - Lee YS
AD  - Division of Life Sciences, Hoseo University, Asan, 31499, South Korea.
FAU - Lee, In Hee
AU  - Lee IH
AD  - Division of Life Sciences, Hoseo University, Asan, 31499, South Korea.
FAU - Kim, Mi-Ae
AU  - Kim MA
AD  - Department of Agricultural Biology, National Academy of Agricultural Science, 
      Rural Development Administration, Wanju, 55365, South Korea.
FAU - Yun, Eun-Young
AU  - Yun EY
AD  - Department of Agricultural Biology, National Academy of Agricultural Science, 
      Rural Development Administration, Wanju, 55365, South Korea.
FAU - Nam, Sung-Hee
AU  - Nam SH
AD  - Department of Agricultural Biology, National Academy of Agricultural Science, 
      Rural Development Administration, Wanju, 55365, South Korea.
FAU - Ahn, Mi-Young
AU  - Ahn MY
AD  - Department of Agricultural Biology, National Academy of Agricultural Science, 
      Rural Development Administration, Wanju, 55365, South Korea.
FAU - Kang, Dongchul
AU  - Kang D
AD  - Ilsong Institute of Life Science, Hallym University, Anyang, 14066, South Korea.
FAU - Hwang, Jae Sam
AU  - Hwang JS
AD  - Department of Agricultural Biology, National Academy of Agricultural Science, 
      Rural Development Administration, Wanju, 55365, South Korea. hwangjs@korea.kr.
LA  - eng
PT  - Journal Article
DEP - 20151002
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Antimicrobial Cationic Peptides)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (CopA3 peptide, Copris tripartitus)
RN  - 0 (Insect Proteins)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antimicrobial Cationic Peptides/chemistry/*pharmacology
MH  - Antineoplastic Agents/chemistry/pharmacology
MH  - Apoptosis/drug effects
MH  - Blotting, Western
MH  - Caspases/metabolism
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Dose-Response Relationship, Drug
MH  - HeLa Cells
MH  - Humans
MH  - Insect Proteins/chemistry/*pharmacology
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Protein Multimerization
MH  - Signal Transduction/drug effects
MH  - Stereoisomerism
MH  - Stomach Neoplasms/*drug therapy/pathology
MH  - Tumor Burden/drug effects
MH  - *Xenograft Model Antitumor Assays
OTO - NOTNLM
OT  - Anticancer peptide
OT  - Apoptosis
OT  - D-CopA3
OT  - Necrosis
OT  - Phosphatidylserine
EDAT- 2015/10/04 06:00
MHDA- 2017/02/12 06:00
CRDT- 2015/10/04 06:00
PHST- 2015/07/03 00:00 [received]
PHST- 2015/09/27 00:00 [accepted]
PHST- 2015/10/04 06:00 [entrez]
PHST- 2015/10/04 06:00 [pubmed]
PHST- 2017/02/12 06:00 [medline]
AID - 10.1007/s13277-015-4162-z [pii]
AID - 10.1007/s13277-015-4162-z [doi]
PST - ppublish
SO  - Tumour Biol. 2016 Mar;37(3):3237-45. doi: 10.1007/s13277-015-4162-z. Epub 2015 
      Oct 2.