PMID- 26432450
OWN - NLM
STAT- MEDLINE
DCOM- 20170116
LR  - 20170116
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 171
IP  - 1
DP  - 2016 May
TI  - The Selenylation Modification of Epimedium Polysaccharide and Isatis Root 
      Polysaccharide and the Immune-enhancing Activity Comparison of Their Modifiers.
PG  - 224-34
LID - 10.1007/s12011-015-0511-4 [doi]
AB  - Epimedium polysaccharide (EPS) and isatis root polysaccharide (IRPS) were 
      extracted, purified, and selenizingly modified by nitric acid-sodium selenite 
      method to obtain nine selenizing EPSs (sEPSs), sEPS1-sEPS9 and nine selenizing 
      IRPSs (sIRPSs), sIRPS1-sIRPS9, respectively. Their effects on chicken peripheral 
      lymphocyte proliferation in vitro were compared by MTT assay. The results showed 
      that selenium polysaccharides at appropriate concentration could promote 
      lymphocyte proliferation more significantly than unmodified polysaccharides, 
      sEPS5 and sIRPS5 with stronger actions were picked out and injected into the 
      chickens vaccinated with Newcastle disease vaccine in vivo tests. The peripheral 
      lymphocyte proliferation and serum antibody titer were determined. The results 
      showed that sEPS5 and sIRPS5 could elevate serum antibody titer and promote 
      lymphocyte proliferation more significantly than unmodified polysaccharides, 
      sEPS5 possessed the strongest efficacy. These results indicate that selenylation 
      modification can significantly enhance the immune-enhancing activity of EPS and 
      IRPS, and sEPS5 can be as a new-type immunopotentiator of chickens.
FAU - Li, Xiuping
AU  - Li X
AD  - Institute of Traditional Chinese Veterinary Medicine, College of veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of 
      China.
AD  - Department of Agriculture, Dezhou University, Dezhou, 253023, People' s Republic 
      of China.
FAU - Hou, Ranran
AU  - Hou R
AD  - Institute of Traditional Chinese Veterinary Medicine, College of veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of 
      China.
FAU - Yue, Chanjuan
AU  - Yue C
AD  - Institute of Traditional Chinese Veterinary Medicine, College of veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of 
      China.
FAU - Liu, Jie
AU  - Liu J
AD  - Institute of Traditional Chinese Veterinary Medicine, College of veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of 
      China.
FAU - Gao, Zhenzhen
AU  - Gao Z
AD  - Institute of Traditional Chinese Veterinary Medicine, College of veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of 
      China.
FAU - Chen, Jin
AU  - Chen J
AD  - National Research Center of Veterinary Biological Engineering and Technology, 
      Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People' s Republic of 
      China.
FAU - Lu, Yu
AU  - Lu Y
AD  - National Research Center of Veterinary Biological Engineering and Technology, 
      Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People' s Republic of 
      China.
FAU - Wang, Deyun
AU  - Wang D
AD  - Institute of Traditional Chinese Veterinary Medicine, College of veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of 
      China.
FAU - Liu, Cui
AU  - Liu C
AD  - Institute of Traditional Chinese Veterinary Medicine, College of veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of 
      China.
FAU - Hu, Yuanliang
AU  - Hu Y
AD  - Institute of Traditional Chinese Veterinary Medicine, College of veterinary 
      Medicine, Nanjing Agricultural University, Nanjing, 210095, People' s Republic of 
      China. ylhu@njau.edu.cn.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151003
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Polysaccharides)
RN  - HIW548RQ3W (Sodium Selenite)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Chickens/*immunology
MH  - Epimedium/*chemistry
MH  - Isatis/*chemistry
MH  - Lymphocytes/drug effects
MH  - Male
MH  - Plant Roots/*chemistry
MH  - Polysaccharides/administration & dosage/*chemistry/*immunology/isolation & 
      purification
MH  - Sodium Selenite/*chemistry/pharmacology
OTO - NOTNLM
OT  - Antibody titer
OT  - Epimedium polysaccharide
OT  - Isatis root polysaccharide
OT  - Lymphocyte proliferation
OT  - Selenylation modification
EDAT- 2015/10/04 06:00
MHDA- 2017/01/17 06:00
CRDT- 2015/10/04 06:00
PHST- 2015/06/15 00:00 [received]
PHST- 2015/09/09 00:00 [accepted]
PHST- 2015/10/04 06:00 [entrez]
PHST- 2015/10/04 06:00 [pubmed]
PHST- 2017/01/17 06:00 [medline]
AID - 10.1007/s12011-015-0511-4 [pii]
AID - 10.1007/s12011-015-0511-4 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2016 May;171(1):224-34. doi: 10.1007/s12011-015-0511-4. Epub 
      2015 Oct 3.