PMID- 26432534
OWN - NLM
STAT- MEDLINE
DCOM- 20160919
LR  - 20181202
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 144
DP  - 2016 Feb
TI  - Responses of the CYP1A biomarker in Jenynsia multidentata and Phalloceros 
      caudimaculatus and evaluation of a CYP1A refractory phenotype.
PG  - 925-31
LID - S0045-6535(15)30059-X [pii]
LID - 10.1016/j.chemosphere.2015.08.050 [doi]
AB  - The level of cytochrome P450 1A (CYP1A) in fish is used as a typical 
      environmental biomarker for the presence of organic contaminants. We used RT-qPCR 
      to investigate CYP1A mRNA levels in the liver, gill and gonopodium of guppies 
      Jenynsia multidentata and Phalloceros caudimaculatus in wetlands within the Rio 
      Grande city (RG) which is under the influence of the Patos Lagoon Estuary (RS, 
      Brazil). The CYP1A mRNA levels evaluated in fish liver from two locations that 
      receive non-treated wastewater effluents (S3 and S4) and another locations near 
      an oil refinery (S6) and an industrial complex (S7), were higher than in 
      locations remote from those sites (S1, S2 and S5). The sum of 16 priority PAHs in 
      sediment confirmed high levels in S4 and S6 (3914.0 and 4414.0 ng g(-1) dw, 
      respectively) comparing to S7>S2>S3>S5>S1 (119.3, 66.3, 62.8, 16.4 and 1.7 ng 
      g(-1) dw). J. multidentata from sites S1 to S4 that were transferred to the 
      laboratory exhibited CYP1A induction after 24 h waterborne exposure to 1 microM 
      betanaphtoflavone (BNF) in all organs compared to controls, except in the liver 
      of fish from site S4. This lack of CYP1A induction by BNF indicates a CYP1A 
      refractory phenotype in guppy. Although this characteristic possibly involves the 
      alteration in AHR signaling or control, the mechanism of resistance is unknown. 
      The present study provides information about the use of the use of CYP1A in South 
      American guppies as an useful biomarker tool for environmental contamination 
      studies.
CI  - Copyright (c) 2015. Published by Elsevier Ltd.
FAU - Chivittz, Cintia C
AU  - Chivittz CC
AD  - Programa de Pos-graduacao em Biologia de Ambientes Aquaticos Continentais, 
      Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio 
      Grande, RS 96203-900, Brazil.
FAU - Pinto, Debora P
AU  - Pinto DP
AD  - Programa de Pos-graduacao em Biologia de Ambientes Aquaticos Continentais, 
      Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio 
      Grande, RS 96203-900, Brazil.
FAU - Ferreira, Roger S
AU  - Ferreira RS
AD  - Programa de Pos-graduacao em Biologia de Ambientes Aquaticos Continentais, 
      Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio 
      Grande, RS 96203-900, Brazil.
FAU - Sopezki, Mauricio da S
AU  - Sopezki Mda S
AD  - Programa de Pos-graduacao em Ciencias Fisiologicas - Fisiologia Animal Comparada, 
      Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio 
      Grande, RS 96203-900, Brazil.
FAU - Fillmann, Gilberto
AU  - Fillmann G
AD  - Instituto de Oceanografia, Universidade Federal do Rio Grande - FURG, Rio Grande, 
      RS 96203-900, Brazil.
FAU - Zanette, Juliano
AU  - Zanette J
AD  - Programa de Pos-graduacao em Biologia de Ambientes Aquaticos Continentais, 
      Instituto de Ciencias Biologicas, Universidade Federal do Rio Grande - FURG, Rio 
      Grande, RS 96203-900, Brazil; Programa de Pos-graduacao em Ciencias Fisiologicas 
      - Fisiologia Animal Comparada, Instituto de Ciencias Biologicas, Universidade 
      Federal do Rio Grande - FURG, Rio Grande, RS 96203-900, Brazil. Electronic 
      address: biozanette@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150929
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Biomarkers)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animal Fins/drug effects/enzymology
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Brazil
MH  - Cyprinodontiformes/genetics/*metabolism
MH  - Cytochrome P-450 CYP1A1/genetics/*metabolism
MH  - Environmental Monitoring/*methods
MH  - Gene Expression/drug effects
MH  - Geologic Sediments/chemistry
MH  - Gills/drug effects/enzymology
MH  - Liver/drug effects/enzymology
MH  - Male
MH  - Phenotype
MH  - Polycyclic Aromatic Hydrocarbons/*analysis/toxicity
MH  - RNA, Messenger/genetics/metabolism
MH  - Seawater/chemistry
MH  - Water Pollutants, Chemical/*analysis/toxicity
OTO - NOTNLM
OT  - Cytochrome P450
OT  - Fish
OT  - Guppy
OT  - PAH
OT  - Patos Lagoon Estuary
OT  - Pollution
EDAT- 2015/10/04 06:00
MHDA- 2016/09/20 06:00
CRDT- 2015/10/04 06:00
PHST- 2015/04/07 00:00 [received]
PHST- 2015/08/14 00:00 [revised]
PHST- 2015/08/18 00:00 [accepted]
PHST- 2015/10/04 06:00 [entrez]
PHST- 2015/10/04 06:00 [pubmed]
PHST- 2016/09/20 06:00 [medline]
AID - S0045-6535(15)30059-X [pii]
AID - 10.1016/j.chemosphere.2015.08.050 [doi]
PST - ppublish
SO  - Chemosphere. 2016 Feb;144:925-31. doi: 10.1016/j.chemosphere.2015.08.050. Epub 
      2015 Sep 29.