PMID- 26433266
OWN - NLM
STAT- MEDLINE
DCOM- 20160516
LR  - 20181202
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1418
DP  - 2015 Oct 30
TI  - Development of a subcritical water extraction approach for trace analysis of 
      chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry 
      tissues.
PG  - 29-35
LID - S0021-9673(15)01353-9 [pii]
LID - 10.1016/j.chroma.2015.09.047 [doi]
AB  - Subcritical water extraction was investigated as a novel and alternative 
      technology for the separation of trace amounts of chloramphenicol, thiamphenicol, 
      florfenicol and its major metabolite florfenicol amine from poultry tissues and 
      its results were compared with those of conventional shaking extraction, 
      ultrasonic extraction, and pressurized liquid extraction. Decreasing the polarity 
      of water by successively increasing the extraction temperature from 50 degrees C to 200 degrees C 
      at the moderate pressure enabled selective, highly effective extractions to be 
      performed. Rapid quantification of the target compounds was carried out by 
      ultra-performance liquid chromatography coupled with electrospray ionization 
      tandem mass spectrometry (UPLC-ESI-MS/MS). The critical parameters of subcritical 
      water extraction such as solvent modifier, temperature, pressure, extraction 
      time, and static cycles were varied with control. The optimized extraction 
      procedures using subcritical water as extraction solvent, were carried out on a 
      pressurized liquid extractor operated at 150 degrees C and 100bar, applying two static 
      cycles for 3min. Average recoveries of the four analytes from fortified samples 
      ranged between 86.8% and 101.5%, with relative standard deviations (RSDs) lower 
      than 7.7%. The limits of detection (LODs) and quantification (LOQs) for the 
      target compounds were in the ranges of 0.03-0.5mugkg(-1) and 0.1-2.0mugkg(-1), 
      respectively. The proposed method is fast, sensitive, water-based thus more 
      environmental acceptable, making it a suitable replacement for conventional 
      organic solvent extraction in veterinary drug residue analysis.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Xiao, Zhiming
AU  - Xiao Z
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Song, Rong
AU  - Song R
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Rao, Zhenghua
AU  - Rao Z
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Wei, Shulin
AU  - Wei S
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Jia, Zheng
AU  - Jia Z
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Suo, Decheng
AU  - Suo D
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Beijing 100081, China.
FAU - Fan, Xia
AU  - Fan X
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese 
      Academy of Agricultural Sciences, Beijing 100081, China. Electronic address: 
      fanxia@caas.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150924
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Solvents)
RN  - 0 (Veterinary Drugs)
RN  - 66974FR9Q1 (Chloramphenicol)
RN  - 76639-93-5 (florfenicol amine)
RN  - 9J97307Y1H (florfenicol)
RN  - FLQ7571NPM (Thiamphenicol)
SB  - IM
MH  - Animals
MH  - Chloramphenicol/*analysis
MH  - Chromatography, High Pressure Liquid/methods
MH  - Drug Residues/analysis
MH  - Limit of Detection
MH  - *Poultry
MH  - Solid Phase Extraction/*methods
MH  - Solvents
MH  - Spectrometry, Mass, Electrospray Ionization/methods
MH  - Tandem Mass Spectrometry/methods
MH  - Thiamphenicol/*analogs & derivatives/analysis
MH  - Veterinary Drugs/*analysis
OTO - NOTNLM
OT  - Chloramphenicol
OT  - Florfenicol
OT  - Florfenicol amine
OT  - Poultry tissues
OT  - Subcritical water extraction
OT  - Thiamphenicol
EDAT- 2015/10/05 06:00
MHDA- 2016/05/18 06:00
CRDT- 2015/10/05 06:00
PHST- 2015/05/24 00:00 [received]
PHST- 2015/09/03 00:00 [revised]
PHST- 2015/09/16 00:00 [accepted]
PHST- 2015/10/05 06:00 [entrez]
PHST- 2015/10/05 06:00 [pubmed]
PHST- 2016/05/18 06:00 [medline]
AID - S0021-9673(15)01353-9 [pii]
AID - 10.1016/j.chroma.2015.09.047 [doi]
PST - ppublish
SO  - J Chromatogr A. 2015 Oct 30;1418:29-35. doi: 10.1016/j.chroma.2015.09.047. Epub 
      2015 Sep 24.