PMID- 26436511
OWN - NLM
STAT- MEDLINE
DCOM- 20160718
LR  - 20151006
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 4
DP  - 2015 Oct 2
TI  - Short Communication: Elucidation of bacterial community structure on thin-spined 
      porcupine (Chaetomys subspinosus) spines by denaturing.
PG  - 11867-75
LID - 10.4238/2015.October.2.20 [doi]
AB  - Thin-spined porcupines (Chaetomys subspinosus) are threatened with extinction and 
      are categorized as vulnerable. This is because of alteration to and loss of their 
      habitat and possible hunting activities in their distribution area. Their spines 
      constitute one of their defense mechanisms, which can be fomites for pathogens to 
      humans. However, little is known about such pathogens. The present study aimed to 
      detect bacteria on spines of C. subspinosus, from the Una Biological Reserve, 
      South of Bahia, northeastern Brazil, by analyzing metagenomic DNA, isolating 
      bacterial culture, using the denaturing gradient gel electrophoresis (DGGE) 
      technique, and sequencing. Six anatomical points were selected for withdrawing 
      spine samples from an individual C. subspinosus. At all sample points, bacteria 
      were detected by bacteriological culture and/or DGGE and sequencing of excised 
      bands. When all samples were combined, standard PCR-DGGE analysis of bacteria 
      present in the spines identified 15 distinct bands, thereby revealing a distinct 
      bacterial community. The main pathogens identified through sequencing were 
      Bacillus cereus, B. thuringiensis, B. anthracis, and B. pumilus. The present 
      study demonstrated the isolation and identification of non-pathogenic and 
      pathogenic bacteria on the spines of C. subspinosus.
FAU - Bezerra, R A
AU  - Bezerra RA
AD  - Programa de Pos-Graduacao em Genetica e Biologia Molecular, Departamento de 
      Ciencias Biologicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brasil 
      rodrigoalvesbezerra@gmail.com.
FAU - Gine, G A F
AU  - Gine GA
AD  - Programa de Pos-Graduacao em Ecologia e Conservacao da Biodiversidade, 
      Departamento de Ciencias Biologicas, Universidade Estadual de Santa Cruz, Ilheus, 
      BA, Brasil.
FAU - Marques, E L S
AU  - Marques EL
AD  - Programa de Pos-Graduacao em Genetica e Biologia Molecular, Departamento de 
      Ciencias Biologicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brasil.
FAU - Abreu-Tarazi, M F
AU  - Abreu-Tarazi MF
AD  - Programa de Pos-Graduacao em Genetica e Biologia Molecular, Departamento de 
      Ciencias Biologicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brasil.
FAU - Rezende, R P
AU  - Rezende RP
AD  - Programa de Pos-Graduacao em Genetica e Biologia Molecular, Departamento de 
      Ciencias Biologicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brasil.
FAU - Gaiotto, F A
AU  - Gaiotto FA
AD  - Programa de Pos-Graduacao em Genetica e Biologia Molecular, Departamento de 
      Ciencias Biologicas, Universidade Estadual de Santa Cruz, Ilheus, BA, Brasil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151002
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacillus/classification/genetics
MH  - Bacillus anthracis/classification/genetics
MH  - Bacillus cereus/classification/genetics
MH  - Bacillus thuringiensis/classification/genetics
MH  - Bacteria/classification/*genetics
MH  - DNA, Bacterial/*genetics
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Endangered Species
MH  - *Metagenome
MH  - Microbial Consortia/genetics
MH  - *Phylogeny
MH  - Polymerase Chain Reaction
MH  - Porcupines/*microbiology
MH  - RNA, Ribosomal, 16S/*genetics
EDAT- 2015/10/06 06:00
MHDA- 2016/07/19 06:00
CRDT- 2015/10/06 06:00
PHST- 2015/10/06 06:00 [entrez]
PHST- 2015/10/06 06:00 [pubmed]
PHST- 2016/07/19 06:00 [medline]
AID - gmr6783 [pii]
AID - 10.4238/2015.October.2.20 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Oct 2;14(4):11867-75. doi: 10.4238/2015.October.2.20.