PMID- 26444015 OWN - NLM STAT- MEDLINE DCOM- 20160602 LR - 20221207 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 10 IP - 10 DP - 2015 TI - The Significance of Myriophyllum elatinoides for Swine Wastewater Treatment: Abundance and Community Structure of Ammonia-Oxidizing Microorganisms in Sediments. PG - e0139778 LID - 10.1371/journal.pone.0139778 [doi] LID - e0139778 AB - Myriophyllum elatinoides was reported to effectively treat wastewater by removing nitrogen (N) and phosphorus (P). However, little is known about the abundance and community structure of ammonia-oxidizing microorganisms associated with M. elatinoides purification systems. The objective of this research was to characterize the abundance and community structure of ammonia-oxidizing microorganisms in swine wastewater and determine the main nitrogen removal pathways. In this study, five different waters were treated by M. elatinoides in microcosms for one month. The five waters included tap water (Control), swine wastewater (SW), 50% diluted swine wastewater (50% SW), and two synthetic wastewaters: 200 mg NH4+-N L(-1) (200 NH4+-N) and 400 mg NH4+-N L(-1) (400 NH4+-N). The most dramatic changes were in NH4+-N and total N (TN) concentrations, with average removal rates of 84% and 90%, respectively, in the treatments containing swine wastewater. On days 7, 14, and 28, the dissolved oxygen (DO) increased by 81.8%, 210.4% and 136.5%, respectively, compared with on day 0, in the swine wastewater. The results also showed that the bacterial amoA (AOB) copy numbers in the sediments of the treatments were significantly higher than those of archaeal amoA (AOA) copy numbers (p = 0.015). In addition, the high DO concentrations in swine wastewater responded well to the high abundance of AOB. The AOA and AOB community distributions were positively related with NO3-N and were negatively related with DO in swine wastewater treatments. In summary, our experimental results suggested that the M. elatinoides purification system could improve the activity of ammonia-oxidizing microorganisms and consequently might contribute to the significant N removal from the swine wastewater. FAU - Li, Xi AU - Li X AD - Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China. FAU - Zhang, Miaomiao AU - Zhang M AD - Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China. FAU - Liu, Feng AU - Liu F AD - Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China. FAU - Li, Yong AU - Li Y AD - Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China. FAU - He, Yang AU - He Y AD - Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Graduate University of Chinese Academy of Sciences, Beijing, 100039, P. R. China. FAU - Zhang, Shunan AU - Zhang S AD - Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Graduate University of Chinese Academy of Sciences, Beijing, 100039, P. R. China. FAU - Wu, Jinshui AU - Wu J AD - Key Laboratory of Agro-ecological Processes in Subtropical Regions, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China; Changsha Research Station for Agricultural & Environmental Monitoring, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan, 410125, P. R. China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151007 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Archaeal Proteins) RN - 0 (Waste Water) RN - 7664-41-7 (Ammonia) RN - EC 1.- (Oxidoreductases) RN - N762921K75 (Nitrogen) SB - IM MH - Ammonia/metabolism MH - Animals MH - Archaea/genetics/*metabolism MH - Archaeal Proteins/genetics/metabolism MH - Bacteria/genetics/*metabolism MH - Ecosystem MH - Geologic Sediments/microbiology MH - Magnoliopsida/*metabolism MH - Nitrogen/metabolism MH - Oxidation-Reduction MH - Oxidoreductases/metabolism MH - Swine MH - Wastewater/*microbiology PMC - PMC4596523 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2015/10/09 06:00 MHDA- 2016/06/03 06:00 PMCR- 2015/10/07 CRDT- 2015/10/08 06:00 PHST- 2015/06/10 00:00 [received] PHST- 2015/09/17 00:00 [accepted] PHST- 2015/10/08 06:00 [entrez] PHST- 2015/10/09 06:00 [pubmed] PHST- 2016/06/03 06:00 [medline] PHST- 2015/10/07 00:00 [pmc-release] AID - PONE-D-15-25395 [pii] AID - 10.1371/journal.pone.0139778 [doi] PST - epublish SO - PLoS One. 2015 Oct 7;10(10):e0139778. doi: 10.1371/journal.pone.0139778. eCollection 2015.