PMID- 26444612
OWN - NLM
STAT- MEDLINE
DCOM- 20170216
LR  - 20220321
IS  - 1875-8622 (Electronic)
IS  - 1386-0291 (Linking)
VI  - 63
IP  - 1
DP  - 2015 Sep 25
TI  - MicroRNA-761 inhibits Angiotensin II-induced vascular smooth muscle cell 
      proliferation and migration by targeting mammalian target of rapamycin.
PG  - 45-56
LID - 10.3233/CH-151981 [doi]
AB  - Aberrant vascular smooth muscle cell (VSMC) proliferation and migration are a 
      major pathological phenomenon in vascular disease characterized by intimal 
      thickening. The important role of the mammalian target of rapamycin (mTOR) 
      signaling in VSMC proliferation has been previously reported. Consequently, 
      down-regulation of mTOR pathway may be an effective way of controlling excessive 
      VSMC proliferation. Since microRNAs (miRNA) are newly emerging regulators of 
      virtually all the biological processes including cellular proliferation, miRNAs 
      targeting mTOR pathway may be utilized to suppress aberrant VSMC proliferation 
      during pathologic conditions. Thus, in the present study, we screened miRNAs 
      targeting mTOR, and we identified miR-761 as a new mTOR targeting miRNA. 
      Luciferase assay using luciferase vector containing 3'UTR of mTOR indicated that 
      miR-761 directly targets mTOR mRNA leading to suppression of mTOR protein 
      expression. Our data also indicate that miR-761 expression decreases during 
      angiotensin II (AngII)-induced proliferation of VSMCs, and exogenous miR-761 
      delivery effectively inhibit the AngII-induced VSMC proliferation. Additionally, 
      the results of migration tests demonstrate that down-regulation of mTOR using 
      exogenous miR-761 suppresses AngII-induced migration of VSMCs as well. Taken 
      together, the present study provided evidence that miR-761 can be a potent 
      anti-proliferative agent for vascular diseases such as atherosclerosis and 
      restenosis, and warrants further studies to validate the effectiveness of miR-761 
      in vivo.
FAU - Cho, Jung Rae
AU  - Cho JR
AD  - Department of Medicine, The Graduate School, Yonsei University, Seoul, Korea.
AD  - Cardiology Division, Kangnam Sacred Heart Hospital, Hallym University College of 
      Medicine, Seoul, Korea.
FAU - Lee, Chang Yeon
AU  - Lee CY
AD  - Department of Integrated Omics for Biomedical Sciences, Yonsei University, Seoul, 
      Korea.
FAU - Lee, Jiyun
AU  - Lee J
AD  - Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Korea.
FAU - Seo, Hyang-Hee
AU  - Seo HH
AD  - Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Korea.
FAU - Choi, Eunhyun
AU  - Choi E
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Korea.
AD  - Catholic Kwandong University International St. Mary's Hospital, Incheon 
      Metropolitan City, Korea.
FAU - Chung, Namsik
AU  - Chung N
AD  - Department of Medicine, The Graduate School, Yonsei University, Seoul, Korea.
FAU - Kim, Sung-Man
AU  - Kim SM
AD  - Catholic Kwandong University International St. Mary's Hospital, Incheon 
      Metropolitan City, Korea.
FAU - Hwang, Ki-Chul
AU  - Hwang KC
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Korea.
AD  - Catholic Kwandong University International St. Mary's Hospital, Incheon 
      Metropolitan City, Korea.
FAU - Lee, Seahyoung
AU  - Lee S
AD  - Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong 
      University, Gangneung-si, Gangwon-do, Korea.
AD  - Catholic Kwandong University International St. Mary's Hospital, Incheon 
      Metropolitan City, Korea.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Clin Hemorheol Microcirc
JT  - Clinical hemorheology and microcirculation
JID - 9709206
RN  - 0 (MIRN761 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 11128-99-7 (Angiotensin II)
RN  - EC 2.7.1.1 (mTOR protein, rat)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Angiotensin II/genetics/*metabolism
MH  - Animals
MH  - Cell Proliferation/physiology
MH  - MicroRNAs/*administration & dosage/genetics/metabolism
MH  - Muscle, Smooth, Vascular/*cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/genetics/*metabolism
MH  - Transfection
OTO - NOTNLM
OT  - Ang II
OT  - VSMC
OT  - mTOR
OT  - miR-761
OT  - proliferation
EDAT- 2015/10/08 06:00
MHDA- 2017/02/17 06:00
CRDT- 2015/10/08 06:00
PHST- 2015/10/08 06:00 [entrez]
PHST- 2015/10/08 06:00 [pubmed]
PHST- 2017/02/17 06:00 [medline]
AID - CH1981 [pii]
AID - 10.3233/CH-151981 [doi]
PST - ppublish
SO  - Clin Hemorheol Microcirc. 2015 Sep 25;63(1):45-56. doi: 10.3233/CH-151981.