PMID- 26445099
OWN - NLM
STAT- MEDLINE
DCOM- 20160527
LR  - 20181113
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 13
DP  - 2015 Oct 6
TI  - Gene expression analysis of pig cumulus-oocyte complexes stimulated in vitro with 
      follicle stimulating hormone or epidermal growth factor-like peptides.
PG  - 113
LID - 10.1186/s12958-015-0112-2 [doi]
LID - 113
AB  - BACKGROUND: The gonadotropin-induced resumption of oocyte meiosis in preovulatory 
      follicles is preceded by expression of epidermal growth factor (EGF)-like 
      peptides, amphiregulin (AREG) and epiregulin (EREG), in mural granulosa and 
      cumulus cells. Both the gonadotropins and the EGF-like peptides possess the 
      capacity to stimulate resumption of oocyte meiosis in vitro via activation of a 
      broad signaling network in cumulus cells. To better understand the rapid genomic 
      actions of gonadotropins (FSH) and EGF-like peptides, we analyzed transcriptomes 
      of cumulus cells at 3 h after their stimulation. METHODS: We hybridized aRNA from 
      cumulus cells to a pig oligonucleotide microarray and compared the transcriptomes 
      of FSH- and AREG/EREG-stimulated cumulus cells with untreated control cells and 
      vice versa. The identified over- and underexpressed genes were subjected to 
      functional genomic analysis according to their molecular and cellular functions. 
      The expression pattern of 50 selected genes with a known or potential function in 
      ovarian development was verified by real-time qRT-PCR. RESULTS: Both FSH and 
      AREG/EREG increased the expression of genes associated with regulation of cell 
      proliferation, cell migration, blood coagulation and extracellular matrix 
      remodeling. FSH alone induced the expression of genes involved in inflammatory 
      response and in the response to reactive oxygen species. Moreover, FSH stimulated 
      the expression of genes closely related to some ovulatory events either 
      exclusively or significantly more than AREG/EREG (AREG, ADAMTS1, HAS2, TNFAIP6, 
      PLAUR, PLAT, and HSD17B7). In contrast to AREG/EREG, FSH also increased the 
      expression of genes coding for key transcription factors (CEBPB, FOS, ID1/3, and 
      NR5A2), which may contribute to the differing expression profiles of FSH- and 
      AREG/EREG-treated cumulus cells. CONCLUSIONS: The impact of FSH on cumulus cell 
      gene transcription was higher than the impact of EGF-like factors in terms of the 
      number of cell functions affected as well as the number of over- and 
      underexpressed genes. Both FSH and EGF-like factors overexpressed genes involved 
      in the post-ovulatory switch in steroidogenesis and tissue remodelling. However, 
      FSH was remarkably more efficient in the up-regulation of several specific genes 
      essential for ovulation of matured oocytes and also genes that been reported to 
      play an important role in maturation of cumulus-enclosed oocytes in vitro.
FAU - Blaha, Milan
AU  - Blaha M
AD  - Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, 
      The Czech Academy of Sciences, Rumburska 89, 277 21, Libechov, Czech Republic.
FAU - Nemcova, Lucie
AU  - Nemcova L
AD  - Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, 
      The Czech Academy of Sciences, Rumburska 89, 277 21, Libechov, Czech Republic.
FAU - Kepkova, Katerina Vodickova
AU  - Kepkova KV
AD  - Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, 
      The Czech Academy of Sciences, Rumburska 89, 277 21, Libechov, Czech Republic.
FAU - Vodicka, Petr
AU  - Vodicka P
AD  - Department of Neurology, Massachusetts General Hospital and Harvard Medical 
      School, Charlestown, MA, USA.
FAU - Prochazka, Radek
AU  - Prochazka R
AD  - Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, 
      The Czech Academy of Sciences, Rumburska 89, 277 21, Libechov, Czech Republic. 
      prochazka@iapg.cas.cz.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151006
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 0 (Amphiregulin)
RN  - 0 (Epiregulin)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Amphiregulin/*pharmacology/physiology
MH  - Animals
MH  - Cells, Cultured
MH  - Cumulus Cells/drug effects/*metabolism
MH  - Epidermal Growth Factor/pharmacology/physiology
MH  - Epiregulin/*pharmacology/physiology
MH  - Female
MH  - Follicle Stimulating Hormone/*pharmacology/physiology
MH  - Gene Expression Regulation
MH  - Oocytes/drug effects/*metabolism
MH  - Swine
PMC - PMC4596359
EDAT- 2015/10/09 06:00
MHDA- 2016/05/28 06:00
PMCR- 2015/10/06
CRDT- 2015/10/08 06:00
PHST- 2015/09/08 00:00 [received]
PHST- 2015/10/02 00:00 [accepted]
PHST- 2015/10/08 06:00 [entrez]
PHST- 2015/10/09 06:00 [pubmed]
PHST- 2016/05/28 06:00 [medline]
PHST- 2015/10/06 00:00 [pmc-release]
AID - 10.1186/s12958-015-0112-2 [pii]
AID - 112 [pii]
AID - 10.1186/s12958-015-0112-2 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2015 Oct 6;13:113. doi: 10.1186/s12958-015-0112-2.