PMID- 26445571
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151008
LR  - 20200930
IS  - 1674-8301 (Print)
IS  - 1674-8301 (Linking)
VI  - 29
IP  - 5
DP  - 2015 Sep
TI  - Netrin-1 overexpression in bone marrow mesenchymal stem cells promotes functional 
      recovery in a rat model of peripheral nerve injury.
PG  - 380-9
LID - 10.7555/JBR.29.20140076 [doi]
AB  - Transplantation of bone marrow mesenchymal stem cells (BMSCs) has been developed 
      as a new method of treating diseases of the peripheral nervous system. While 
      netrin-1 is a critical molecule for axonal path finding and nerve growth, it may 
      also affect vascular network formation. Here, we investigated the effect of 
      transplanting BMSCs that produce netrin-1 in a rat model of sciatic nerve crush 
      injury. We introduced a sciatic nerve crush injury, and then injected 1x10(6) 
      BMSCs infected by a recombinant adenovirus expressing netrin-1 Ad5-Netrin-1-EGFP 
      or culture medium into the injured part in the next day. At day 7, 14 and 28 
      after injection, we measured motor nerve conduction and detected mRNA expressions 
      of netrin-1 receptors UNC5B and Deleted in Colorectal Cancer (DCC), and 
      neurotrophic factors brain-derived neurotrophic factor (BDNF) and nerve growth 
      factor (NGF) by real-time PCR. We also detected protein expressions of BDNF and 
      NGF by Western blotting assays and examined BMSCs that incorporated into myelin 
      and vascellum. The results showed that BMSCs infected by Ad5-Netrin-1-EGFP 
      significantly improved the function of the sciatic nerve, and led to increased 
      expression of BDNF and NGF (P<0.05). Moreover, 28 days after injury, more Schwann 
      cells were found in BMSCs infected by Ad5-Netrin-1-EGFP compared to control 
      BMSCs. In conclusion, transplantation of BMSCs that produce netrin-1 improved the 
      function of the sciatic nerve after injury. This method may be a new treatment of 
      nerve injury.
FAU - Ke, Xianjin
AU  - Ke X
AD  - Department of Neurology, Affiliated Hospital of Jiangsu University , Zhenjiang, 
      Jiangsu 212001 , China .
FAU - Li, Qian
AU  - Li Q
AD  - Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University , 
      Nanjing, Jiangsu 210006 , China .
FAU - Xu, Li
AU  - Xu L
AD  - Department of Neurology, Affiliated Hospital of Jiangsu University , Zhenjiang, 
      Jiangsu 212001 , China .
FAU - Zhang, Ying
AU  - Zhang Y
AD  - Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University , 
      Nanjing, Jiangsu 210006 , China .
FAU - Li, Dongmei
AU  - Li D
AD  - Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University , 
      Nanjing, Jiangsu 210006 , China .
FAU - Ma, Jianhua
AU  - Ma J
AD  - Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University , 
      Nanjing, Jiangsu 210006 , China .
FAU - Mao, Xiaoming
AU  - Mao X
AD  - Department of Endocrinology, Nanjing First Hospital, Nanjing Medical University , 
      Nanjing, Jiangsu 210006 , China .
LA  - eng
PT  - Journal Article
DEP - 20150730
PL  - China
TA  - J Biomed Res
JT  - Journal of biomedical research
JID - 101551157
PMC - PMC4585432
OTO - NOTNLM
OT  - Deleted in Colorectal Cancer
OT  - UNC5B
OT  - bone marrow mesenchymal stem cells
OT  - brain-derived neurotrophic factor
OT  - nerve growth factor
OT  - netrin-1
COIS- CLC number: R745, Document code: A The authors reported no conflict of interests.
EDAT- 2015/10/09 06:00
MHDA- 2015/10/09 06:01
PMCR- 2015/09/01
CRDT- 2015/10/08 06:00
PHST- 2014/05/16 00:00 [received]
PHST- 2014/10/01 00:00 [revised]
PHST- 2015/04/08 00:00 [accepted]
PHST- 2015/10/08 06:00 [entrez]
PHST- 2015/10/09 06:00 [pubmed]
PHST- 2015/10/09 06:01 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - jbr-29-05-380 [pii]
AID - 10.7555/JBR.29.20140076 [doi]
PST - ppublish
SO  - J Biomed Res. 2015 Sep;29(5):380-9. doi: 10.7555/JBR.29.20140076. Epub 2015 Jul 
      30.