PMID- 26446055
OWN - NLM
STAT- MEDLINE
DCOM- 20160701
LR  - 20160211
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 56
IP  - 2
DP  - 2016 Feb
TI  - Platelet components associated with adverse reactions: predictive value of 
      mitochondrial DNA relative to biological response modifiers.
PG  - 497-504
LID - 10.1111/trf.13373 [doi]
AB  - BACKGROUND: Biological response modifiers (BRMs), secreted by platelets (PLTs) 
      during storage, play a role in adverse events (AEs) associated with transfusion. 
      Moreover, mitochondrial DNA (mtDNA) levels in PLT components (PCs) are associated 
      with AEs. In this study we explore whether there is a correlation between 
      pathogenic BRMs and mtDNA levels and whether these markers can be considered 
      predictors of transfusion pathology. STUDY DESIGN AND METHODS: We investigated a 
      series of reported AEs after PC transfusion, combining clinical observations and 
      mathematical modeling systems. RESULTS: mtDNA was consistently released during 
      the first days of PC storage; however, mtDNA release was earlier in "pathogenic" 
      than in nonpathogenic PCs. PC supernatants with high levels of mtDNA along with 
      soluble CD40 ligand (sCD40L) were significantly associated with occurrences of 
      AEs. The fact that mtDNA did not associate with the 14 BRMs tested suggests the 
      role of mtDNA in PC transfusion-linked inflammation is independent of that of 
      BRMs, known to be associated with AEs. We present evidence that PLTs generate 
      distinct pathogenic secretion profiles of BRMs and mtDNA. The calculated area 
      under the curve for mtDNA was significantly associated with AEs, although less 
      stringently predictive than those of sCD40L or interleukin-13, standard 
      predictors of AE. The established model predicts that distinct subtypes of AEs 
      can be distinguished, dependent on mtDNA levels and PC storage length. 
      CONCLUSIONS: Further work should be considered to test the propensity of mtDNA in 
      PLT concentrates to generate inflammation and cause an AE.
CI  - (c) 2015 AABB.
FAU - Cognasse, Fabrice
AU  - Cognasse F
AD  - Etablissement Francais du Sang Auvergne-Loire, Saint-Etienne.
AD  - GIMAP-EA3064, Universite de Lyon, Saint-Etienne, France.
FAU - Aloui, Chaker
AU  - Aloui C
AD  - GIMAP-EA3064, Universite de Lyon, Saint-Etienne, France.
FAU - Anh Nguyen, Kim
AU  - Anh Nguyen K
AD  - GIMAP-EA3064, Universite de Lyon, Saint-Etienne, France.
FAU - Hamzeh-Cognasse, Hind
AU  - Hamzeh-Cognasse H
AD  - GIMAP-EA3064, Universite de Lyon, Saint-Etienne, France.
FAU - Fagan, Jocelyne
AU  - Fagan J
AD  - Etablissement Francais du Sang Auvergne-Loire, Saint-Etienne.
FAU - Arthaud, Charles-Antoine
AU  - Arthaud CA
AD  - Etablissement Francais du Sang Auvergne-Loire, Saint-Etienne.
FAU - Eyraud, Marie-Ange
AU  - Eyraud MA
AD  - Etablissement Francais du Sang Auvergne-Loire, Saint-Etienne.
FAU - Sebban, Marc
AU  - Sebban M
AD  - Laboratoire Hubert Curien, UMR CNRS 5516, Saint-Etienne.
FAU - Fromont, Elisa
AU  - Fromont E
AD  - Laboratoire Hubert Curien, UMR CNRS 5516, Saint-Etienne.
FAU - Pozzetto, Bruno
AU  - Pozzetto B
AD  - GIMAP-EA3064, Universite de Lyon, Saint-Etienne, France.
FAU - Laradi, Sandrine
AU  - Laradi S
AD  - Etablissement Francais du Sang Auvergne-Loire, Saint-Etienne.
AD  - GIMAP-EA3064, Universite de Lyon, Saint-Etienne, France.
FAU - Garraud, Olivier
AU  - Garraud O
AD  - GIMAP-EA3064, Universite de Lyon, Saint-Etienne, France.
AD  - INTS-Institut National de la Transfusion Sanguine, Paris, France.
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20151007
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (Interleukin-13)
RN  - 147205-72-9 (CD40 Ligand)
SB  - IM
MH  - Blood Platelets/*metabolism
MH  - Blood Preservation/*adverse effects
MH  - CD40 Ligand/*metabolism
MH  - DNA, Mitochondrial/*metabolism
MH  - Female
MH  - Humans
MH  - Interleukin-13/*metabolism
MH  - Male
MH  - Platelet Transfusion/*adverse effects
MH  - Time Factors
EDAT- 2015/10/09 06:00
MHDA- 2016/07/02 06:00
CRDT- 2015/10/09 06:00
PHST- 2015/05/12 00:00 [received]
PHST- 2015/07/23 00:00 [revised]
PHST- 2015/08/31 00:00 [accepted]
PHST- 2015/10/09 06:00 [entrez]
PHST- 2015/10/09 06:00 [pubmed]
PHST- 2016/07/02 06:00 [medline]
AID - 10.1111/trf.13373 [doi]
PST - ppublish
SO  - Transfusion. 2016 Feb;56(2):497-504. doi: 10.1111/trf.13373. Epub 2015 Oct 7.