PMID- 26446775
OWN - NLM
STAT- MEDLINE
DCOM- 20160819
LR  - 20151015
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Linking)
VI  - 7
IP  - 41
DP  - 2015 Nov 7
TI  - Highly sensitive detection of DNA methylation levels by using a quantum dot-based 
      FRET method.
PG  - 17547-55
LID - 10.1039/c5nr04956c [doi]
AB  - DNA methylation is the most frequently studied epigenetic modification that is 
      strongly involved in genomic stability and cellular plasticity. Aberrant changes 
      in DNA methylation status are ubiquitous in human cancer and the detection of 
      these changes can be informative for cancer diagnosis. Herein, we reported a 
      facile quantum dot-based (QD-based) fluorescence resonance energy transfer (FRET) 
      technique for the detection of DNA methylation. The method relies on 
      methylation-sensitive restriction enzymes for the differential digestion of 
      genomic DNA based on its methylation status. Digested DNA is then subjected to 
      PCR amplification for the incorporation of Alexa Fluor-647 (A647) fluorophores. 
      DNA methylation levels can be detected qualitatively through gel analysis and 
      quantitatively by the signal amplification from QDs to A647 during FRET. 
      Furthermore, the methylation levels of three tumor suppressor genes, PCDHGB6, 
      HOXA9 and RASSF1A, in 20 lung adenocarcinoma and 20 corresponding adjacent 
      nontumorous tissue (NT) samples were measured to verify the feasibility of the 
      QD-based FRET method and a high sensitivity for cancer detection (up to 90%) was 
      achieved. Our QD-based FRET method is a convenient, continuous and 
      high-throughput method, and is expected to be an alternative for detecting DNA 
      methylation as a biomarker for certain human cancers.
FAU - Ma, Yunfei
AU  - Ma Y
AD  - Institute of Applied Chemistry, East China University of Science and Technology, 
      Shanghai, 200237, PR China. zhongxh@ecust.edu.cn.
FAU - Zhang, Honglian
AU  - Zhang H
FAU - Liu, Fangming
AU  - Liu F
FAU - Wu, Zhenhua
AU  - Wu Z
FAU - Lu, Shaohua
AU  - Lu S
FAU - Jin, Qinghui
AU  - Jin Q
FAU - Zhao, Jianlong
AU  - Zhao J
FAU - Zhong, Xinhua
AU  - Zhong X
FAU - Mao, Hongju
AU  - Mao H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
RN  - 0 (Alexa Fluor 647)
RN  - 0 (Carbocyanines)
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adenocarcinoma/*metabolism/pathology
MH  - Carbocyanines/*chemistry
MH  - Cell Line, Tumor
MH  - *DNA Methylation
MH  - DNA, Neoplasm/*metabolism
MH  - Fluorescence Resonance Energy Transfer/*methods
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - Lung Neoplasms/*metabolism/pathology
MH  - Quantum Dots/*chemistry
EDAT- 2015/10/09 06:00
MHDA- 2016/08/20 06:00
CRDT- 2015/10/09 06:00
PHST- 2015/10/09 06:00 [entrez]
PHST- 2015/10/09 06:00 [pubmed]
PHST- 2016/08/20 06:00 [medline]
AID - 10.1039/c5nr04956c [doi]
PST - ppublish
SO  - Nanoscale. 2015 Nov 7;7(41):17547-55. doi: 10.1039/c5nr04956c.