PMID- 26447559
OWN - NLM
STAT- MEDLINE
DCOM- 20160504
LR  - 20161125
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 63
IP  - 42
DP  - 2015 Oct 28
TI  - Assessment of the Allergenic Potential of Transgenic Wheat (Triticum aestivum) 
      with Reduced Levels of omega5-Gliadins, the Major Sensitizing Allergen in 
      Wheat-Dependent Exercise-Induced Anaphylaxis.
PG  - 9323-32
LID - 10.1021/acs.jafc.5b03557 [doi]
AB  - The omega5-gliadins are the major sensitizing allergens in wheat-dependent 
      exercise-induced anaphylaxis (WDEIA). In this study, two-dimensional immunoblot 
      analysis was used to assess the allergenic potential of two transgenic wheat 
      lines in which omega5-gliadin genes were silenced by RNA interference. Sera from 7 of 
      11 WDEIA patients showed greatly reduced levels of immunoglobulin E (IgE) 
      reactivity to omega5-gliadins in both transgenic lines. However, these sera also 
      showed low levels of reactivity to other gluten proteins. Sera from three 
      patients showed the greatest reactivity to proteins other than omega5-gliadins, 
      either high-molecular-weight glutenin subunits (HMW-GSs), alpha-gliadins, or 
      non-gluten proteins. The complexity of immunological responses among these 
      patients suggests that flour from the transgenic lines would not be suitable for 
      individuals already diagnosed with WDEIA. However, the introduction of wheat 
      lacking omega5-gliadins could reduce the number of people sensitized to these 
      proteins and thereby decrease the overall incidence of this serious food allergy.
FAU - Altenbach, Susan B
AU  - Altenbach SB
AD  - Western Regional Research Center, Agricultural Research Services (ARS), United 
      States Department of Agriculture (USDA) , 800 Buchanan Street Albany, California 
      94710, United States.
FAU - Tanaka, Charlene K
AU  - Tanaka CK
AD  - Western Regional Research Center, Agricultural Research Services (ARS), United 
      States Department of Agriculture (USDA) , 800 Buchanan Street Albany, California 
      94710, United States.
FAU - Pineau, Florence
AU  - Pineau F
AD  - UR1268 Biopolymers, Interactions, Assemblies, Institut National de la Recherche 
      Agronomique (INRA) , Rue de la Geraudiere, F-44316 Nantes Cedex 03, France.
FAU - Lupi, Roberta
AU  - Lupi R
AD  - UR1268 Biopolymers, Interactions, Assemblies, Institut National de la Recherche 
      Agronomique (INRA) , Rue de la Geraudiere, F-44316 Nantes Cedex 03, France.
FAU - Drouet, Martine
AU  - Drouet M
AD  - Unite d'Allergologie Generale et de Pneumologie, Centre Hospitalier Universitaire 
      (CHU) d'Angers , F-49933 Angers, France.
FAU - Beaudouin, Etienne
AU  - Beaudouin E
AD  - Service d'Allergologie, Centre Hospitalier (CH) Epinal , F-88021 Epinal, France.
FAU - Morisset, Martine
AU  - Morisset M
AD  - Immunologie-Allergologie, Centre Hospitalier (CH) de Luxembourg , L-1210 
      Luxembourg, Luxembourg.
FAU - Denery-Papini, Sandra
AU  - Denery-Papini S
AD  - UR1268 Biopolymers, Interactions, Assemblies, Institut National de la Recherche 
      Agronomique (INRA) , Rue de la Geraudiere, F-44316 Nantes Cedex 03, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20151016
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Antigens, Plant)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 8002-80-0 (Glutens)
RN  - 9007-90-3 (Gliadin)
RN  - FX065C7O71 (glutenin)
SB  - IM
MH  - Adult
MH  - Anaphylaxis/blood/*immunology
MH  - Antigens, Plant/analysis/genetics/*immunology
MH  - Exercise
MH  - Female
MH  - Flour/analysis
MH  - Food, Genetically Modified
MH  - Gliadin/analysis/genetics/*immunology
MH  - Glutens/immunology
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Plants, Genetically Modified/chemistry/genetics/*immunology
MH  - Triticum/chemistry/genetics/*immunology
MH  - Wheat Hypersensitivity/blood/*immunology
OTO - NOTNLM
OT  - RNA interference
OT  - Triticum aestivum
OT  - WDEIA
OT  - food allergy
OT  - gene silencing
OT  - gliadins
OT  - gluten proteins
OT  - immunoblots
OT  - wheat
EDAT- 2015/10/09 06:00
MHDA- 2016/05/05 06:00
CRDT- 2015/10/09 06:00
PHST- 2015/10/09 06:00 [entrez]
PHST- 2015/10/09 06:00 [pubmed]
PHST- 2016/05/05 06:00 [medline]
AID - 10.1021/acs.jafc.5b03557 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2015 Oct 28;63(42):9323-32. doi: 10.1021/acs.jafc.5b03557. 
      Epub 2015 Oct 16.