PMID- 26448191
OWN - NLM
STAT- MEDLINE
DCOM- 20160411
LR  - 20201218
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 40
IP  - 1
DP  - 2016 Jan
TI  - Utility of BAP1 Immunohistochemistry and p16 (CDKN2A) FISH in the Diagnosis of 
      Malignant Mesothelioma in Effusion Cytology Specimens.
PG  - 120-6
LID - 10.1097/PAS.0000000000000529 [doi]
AB  - The diagnosis of malignant mesothelioma in effusion cytology specimens is 
      controversial. BAP1 immunohistochemistry and p16 fluorescence in situ 
      hybridization (FISH) have recently been reported as reliable markers of 
      malignancy in biopsies of mesothelioma. To determine whether these markers, 
      singly or in combination, might also be useful in effusion cytology specimens, we 
      examined 15 biopsies of epithelial mesotheliomas and 3 benign mesothelial 
      reactions and corresponding effusion cytology paraffin-embedded cell blocks. Four 
      cytology specimens were too scanty for p16 FISH analysis but were interpretable 
      for BAP1 immunohistochemistry. Overall, loss of BAP1 and/or deletion of p16 was 
      seen in 11/11 (100%) of matched cytology and tissue biopsy specimens. BAP1 loss 
      alone was seen in 10/15 (67%) biopsies and 10/15 (67%) cytology specimens. 
      Homozygous deletion of p16 by FISH was found in 12/15 (80%) biopsy specimens and 
      8/11 (73%) evaluable cytology specimens. Seven of 15 (47%) biopsies and 5/11 
      (42%) cytology specimens showed loss of both markers. All mesothelioma 
      biopsy/cytology pairs showed exactly the same pattern of BAP1 or p16 retention or 
      loss in the biopsy and cytology specimens. The 2 peritoneal mesothelioma cases 
      demonstrated loss of BAP1 but not p16. None of the benign mesothelial reactions 
      or corresponding cytology specimens showed loss of either marker. We conclude 
      that both BAP1 immunohistochemistry and p16 FISH analysis provide reliable 
      markers of mesothelial malignancy in effusion cytology specimens, especially 
      where the atypical mesothelial proliferation is well sampled. BAP1 is easier to 
      interpret with scanty specimens. On the basis of small numbers of cases, use of 
      both markers appears to increase sensitivity.
FAU - Hwang, Harry C
AU  - Hwang HC
AD  - *PhenoPath Laboratories, Seattle, WA daggerDepartment of Pathology, Vancouver General 
      Hospital double daggerDepartment of Pathology, University of British Columbia, Vancouver, BC, 
      Canada.
FAU - Sheffield, Brandon S
AU  - Sheffield BS
FAU - Rodriguez, Stephanie
AU  - Rodriguez S
FAU - Thompson, Kim
AU  - Thompson K
FAU - Tse, Christopher H
AU  - Tse CH
FAU - Gown, Allen M
AU  - Gown AM
FAU - Churg, Andrew
AU  - Churg A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (BAP1 protein, human)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*analysis/*genetics
MH  - Biopsy
MH  - Cell Proliferation
MH  - Cyclin-Dependent Kinase Inhibitor p16/*genetics
MH  - Down-Regulation
MH  - Female
MH  - Gene Deletion
MH  - Humans
MH  - *Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*diagnosis/enzymology/genetics/pathology
MH  - Male
MH  - Mesothelioma/*diagnosis/enzymology/genetics/pathology
MH  - Mesothelioma, Malignant
MH  - Middle Aged
MH  - Paraffin Embedding
MH  - Pleural Effusion, Malignant/*diagnosis/enzymology/genetics/pathology
MH  - Predictive Value of Tests
MH  - Prognosis
MH  - Tumor Suppressor Proteins/*analysis
MH  - Ubiquitin Thiolesterase/*analysis
EDAT- 2015/10/09 06:00
MHDA- 2016/04/12 06:00
CRDT- 2015/10/09 06:00
PHST- 2015/10/09 06:00 [entrez]
PHST- 2015/10/09 06:00 [pubmed]
PHST- 2016/04/12 06:00 [medline]
AID - 10.1097/PAS.0000000000000529 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2016 Jan;40(1):120-6. doi: 10.1097/PAS.0000000000000529.