PMID- 26449454
OWN - NLM
STAT- MEDLINE
DCOM- 20160201
LR  - 20151020
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 467
IP  - 2
DP  - 2015 Nov 13
TI  - Direct fluorescence in situ hybridization on human metaphase chromosomes using 
      quantum dot-platinum labeled DNA probes.
PG  - 328-33
LID - S0006-291X(15)30693-8 [pii]
LID - 10.1016/j.bbrc.2015.09.161 [doi]
AB  - The telomere shortening in chromosomes implies the senescence, apoptosis, or 
      oncogenic transformation of cells. Since detecting telomeres in aging and 
      diseases like cancer, is important, the direct detection of telomeres has been a 
      very useful biomarker. We propose a telomere detection method using a newly 
      synthesized quantum dot (QD) based probe with oligonucleotide conjugation and 
      direct fluorescence in situ hybridization (FISH). QD-oligonucleotides were 
      prepared with metal coordination bonding based on platinum-guanine binding 
      reported in our previous work. The QD-oligonucleotide conjugation method has an 
      advantage where any sequence containing guanine at the end can be easily bound to 
      the starting QD-Pt conjugate. A synthesized telomeric oligonucleotide was bound 
      to the QD-Pt conjugate successfully and this probe hybridized specifically on the 
      telomere of fabricated MV-4-11 and MOLT-4 chromosomes. Additionally, the 
      QD-telomeric oligonucleotide probe successfully detected the telomeres on the CGH 
      metaphase slide. Due to the excellent photostability and high quantum yield of 
      QDs, the QD-oligonucleotide probe has high fluorescence intensity when compared 
      to the organic dye-oligonucleotide probe. Our QD-oligonucleotide probe, 
      conjugation method of this QD probe, and hybridization protocol with the 
      chromosomes can be a useful tool for chromosome painting and FISH.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Hwang, Gyoyeon
AU  - Hwang G
AD  - Chemical Kinomics Research Center, Future Convergence Research Division, Korea 
      Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 
      136-791, Republic of Korea; Biological Chemistry, Korea University of Science and 
      Technology, 217, Gajeong-ro, Yuseong-gu, Deajeon, Republic of Korea.
FAU - Lee, Hansol
AU  - Lee H
AD  - Chemical Kinomics Research Center, Future Convergence Research Division, Korea 
      Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 
      136-791, Republic of Korea.
FAU - Lee, Jiyeon
AU  - Lee J
AD  - Chemical Kinomics Research Center, Future Convergence Research Division, Korea 
      Institute of Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul 
      136-791, Republic of Korea; Biological Chemistry, Korea University of Science and 
      Technology, 217, Gajeong-ro, Yuseong-gu, Deajeon, Republic of Korea. Electronic 
      address: jylee@kist.re.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151009
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA Probes)
RN  - 0 (Oligonucleotides)
RN  - 49DFR088MY (Platinum)
SB  - IM
MH  - Cell Line, Tumor
MH  - Chromosomes, Human/chemistry/*ultrastructure
MH  - DNA Probes/chemical synthesis/*chemistry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/chemistry/ultrastructure
MH  - *Metaphase
MH  - Microscopy, Fluorescence
MH  - Oligonucleotides/chemistry
MH  - Platinum/*chemistry
MH  - Quantum Dots/*chemistry
MH  - Telomere/chemistry/ultrastructure
OTO - NOTNLM
OT  - Chromosome painting
OT  - Fluorescence in situ hybridization (FISH)
OT  - Quantum dot (QD)
OT  - Telomere
EDAT- 2015/10/10 06:00
MHDA- 2016/02/02 06:00
CRDT- 2015/10/10 06:00
PHST- 2015/09/25 00:00 [received]
PHST- 2015/09/30 00:00 [accepted]
PHST- 2015/10/10 06:00 [entrez]
PHST- 2015/10/10 06:00 [pubmed]
PHST- 2016/02/02 06:00 [medline]
AID - S0006-291X(15)30693-8 [pii]
AID - 10.1016/j.bbrc.2015.09.161 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2015 Nov 13;467(2):328-33. doi: 
      10.1016/j.bbrc.2015.09.161. Epub 2015 Oct 9.