PMID- 26449660
OWN - NLM
STAT- MEDLINE
DCOM- 20160722
LR  - 20220408
IS  - 1476-5551 (Electronic)
IS  - 0887-6924 (Linking)
VI  - 30
IP  - 2
DP  - 2016 Feb
TI  - Array-based comparative genomic hybridization detects copy number variations with 
      prognostic relevance in 80% of ALL with normal karyotype or failed chromosome 
      analysis.
PG  - 318-24
LID - 10.1038/leu.2015.276 [doi]
AB  - Pretreatment cytogenetics is an important parameter for risk stratification and 
      therapy approach in acute lymphoblastic leukemia (ALL). However, in up to 30% of 
      cases, chromosome banding analysis (CBA) fails or reveals a normal karyotype. To 
      characterize the subset of ALL with normal karyotype or failed CBA, we performed 
      fluorescence in situ hybridization (FISH) or PCR for BCR-ABL1 and MLL 
      rearrangements as well as array comparative genomic hybridization (aCGH) in 186 
      adult patients. We further carried out FISH for MYC in cases with Burkitt 
      leukemia phenotype. FISH or PCR revealed one of the respective rearrangements in 
      22% of patients. In 80% of cases, copy number variations (CNV) were identified by 
      aCGH. In 22% of cases, all CNV were below the resolution of CBA. On the basis of 
      results of FISH, RT-PCR and aCGH, patients were categorized into three groups. 
      The novel subset of patients with submicroscopic CNV only showed an overall 
      survival at 3 years of 84% compared with 64% for patients classified as adverse 
      abnormalities and 77% for cases with other aberrations (P=0.046). Thus, ALL with 
      non-informative CBA can be further classified by FISH and aCGH providing 
      prognostic information, which may be useful for a more individualized therapy.
FAU - Muhlbacher, V
AU  - Muhlbacher V
AD  - MLL Munich Leukemia Laboratory, Munich, Germany.
FAU - Haferlach, T
AU  - Haferlach T
AD  - MLL Munich Leukemia Laboratory, Munich, Germany.
FAU - Kern, W
AU  - Kern W
AD  - MLL Munich Leukemia Laboratory, Munich, Germany.
FAU - Zenger, M
AU  - Zenger M
AD  - MLL Munich Leukemia Laboratory, Munich, Germany.
FAU - Schnittger, S
AU  - Schnittger S
AD  - MLL Munich Leukemia Laboratory, Munich, Germany.
FAU - Haferlach, C
AU  - Haferlach C
AD  - MLL Munich Leukemia Laboratory, Munich, Germany.
LA  - eng
PT  - Journal Article
DEP - 20151009
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chromosome Banding
MH  - *Comparative Genomic Hybridization
MH  - *DNA Copy Number Variations
MH  - Female
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Male
MH  - Middle Aged
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics/mortality
MH  - Prognosis
EDAT- 2015/10/10 06:00
MHDA- 2016/07/23 06:00
CRDT- 2015/10/10 06:00
PHST- 2015/06/30 00:00 [received]
PHST- 2015/09/09 00:00 [revised]
PHST- 2015/09/30 00:00 [accepted]
PHST- 2015/10/10 06:00 [entrez]
PHST- 2015/10/10 06:00 [pubmed]
PHST- 2016/07/23 06:00 [medline]
AID - leu2015276 [pii]
AID - 10.1038/leu.2015.276 [doi]
PST - ppublish
SO  - Leukemia. 2016 Feb;30(2):318-24. doi: 10.1038/leu.2015.276. Epub 2015 Oct 9.