PMID- 26450925
OWN - NLM
STAT- MEDLINE
DCOM- 20160318
LR  - 20220129
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 472
IP  - 3
DP  - 2015 Dec 15
TI  - Histone deacetylase 3 indirectly modulates tubulin acetylation.
PG  - 367-77
LID - 10.1042/BJ20150660 [doi]
AB  - Histone deacetylase 3 (HDAC3), a member of the Class I subfamily of HDACs, is 
      found in both the nucleus and the cytoplasm. Its roles in the nucleus have been 
      well characterized, but its cytoplasmic roles are still not elucidated fully. We 
      found that blocking HDAC3 activity using MI192, a compound specific for HDAC3, 
      modulated tubulin acetylation in the human prostate cancer cell line PC3. A brief 
      1 h treatment of PC3 cells with MI192 significantly increased levels of tubulin 
      acetylation and ablated the dynamic behaviour of microtubules in live cells. 
      siRNA-mediated knockdown (KD) of HDAC3 in PC3 cells, significantly increased 
      levels of tubulin acetylation, and overexpression reduced it. However, the active 
      HDAC3-silencing mediator of retinoic and thyroid receptors 
      (SMRT)-deacetylase-activating domain (DAD) complex did not directly deacetylate 
      tubulin in vitro. These data suggest that HDAC3 indirectly modulates tubulin 
      acetylation.
CI  - (c) 2015 Authors.
FAU - Bacon, Travis
AU  - Bacon T
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds LS2 9JT, U.K.
FAU - Seiler, Caroline
AU  - Seiler C
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds LS2 9JT, U.K.
FAU - Wolny, Marcin
AU  - Wolny M
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds LS2 9JT, U.K.
FAU - Hughes, Ruth
AU  - Hughes R
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds LS2 9JT, U.K.
FAU - Watson, Peter
AU  - Watson P
AD  - Department of Molecular and Cell Biology, Henry Wellcome Laboratories of 
      Structural Biology, University of Leicester, Lancaster Road, Leicester LE1 9HN, 
      U.K.
FAU - Schwabe, John
AU  - Schwabe J
AD  - Department of Molecular and Cell Biology, Henry Wellcome Laboratories of 
      Structural Biology, University of Leicester, Lancaster Road, Leicester LE1 9HN, 
      U.K.
FAU - Grigg, Ronald
AU  - Grigg R
AD  - School of Chemistry, Faculty of Maths and Physical Sciences, University of Leeds, 
      Leeds LS2 9JT, U.K.
FAU - Peckham, Michelle
AU  - Peckham M
AD  - School of Molecular and Cellular Biology, Faculty of Biological Sciences, 
      University of Leeds, Leeds LS2 9JT, U.K. m.peckham@leeds.ac.uk.
LA  - eng
GR  - 100237/WT_/Wellcome Trust/United Kingdom
GR  - MRC_/Medical Research Council/United Kingdom
GR  - WT104918MA/WT_/Wellcome Trust/United Kingdom
GR  - BB/I007423/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - PG/15/2/31208/BHF_/British Heart Foundation/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151008
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Benzamides)
RN  - 0 (MI-192)
RN  - 0 (NCOR2 protein, human)
RN  - 0 (Nuclear Receptor Co-Repressor 2)
RN  - 0 (Tubulin)
RN  - EC 3.5.1.98 (Histone Deacetylases)
RN  - EC 3.5.1.98 (histone deacetylase 3)
SB  - IM
MH  - Acetylation/drug effects
MH  - Benzamides/pharmacology
MH  - Cell Line, Tumor
MH  - Gene Knockdown Techniques
MH  - Histone Deacetylases/genetics/*metabolism
MH  - Humans
MH  - Microtubules/genetics/*metabolism
MH  - Nuclear Receptor Co-Repressor 2/genetics/*metabolism
MH  - Tubulin/genetics/*metabolism
PMC - PMC4661566
OTO - NOTNLM
OT  - acetylation
OT  - histone deacetylase 3
OT  - microtubules
OT  - tubulin
EDAT- 2015/10/10 06:00
MHDA- 2016/03/19 06:00
PMCR- 2015/11/27
CRDT- 2015/10/10 06:00
PHST- 2015/06/08 00:00 [received]
PHST- 2015/10/08 00:00 [accepted]
PHST- 2015/10/10 06:00 [entrez]
PHST- 2015/10/10 06:00 [pubmed]
PHST- 2016/03/19 06:00 [medline]
PHST- 2015/11/27 00:00 [pmc-release]
AID - BJ20150660 [pii]
AID - 10.1042/BJ20150660 [doi]
PST - ppublish
SO  - Biochem J. 2015 Dec 15;472(3):367-77. doi: 10.1042/BJ20150660. Epub 2015 Oct 8.