PMID- 26452831
OWN - NLM
STAT- MEDLINE
DCOM- 20160718
LR  - 20220321
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 144
DP  - 2015 Nov 1
TI  - Stable isotope labeling assisted liquid chromatography-electrospray tandem mass 
      spectrometry for quantitative analysis of endogenous gibberellins.
PG  - 341-8
LID - S0039-9140(15)30098-9 [pii]
LID - 10.1016/j.talanta.2015.06.056 [doi]
AB  - In the current study, we developed a stable isotope labeling strategy for the 
      absolute quantification of gibberellins (GAs) by high performance liquid 
      chromatography-electrospray tandem mass spectrometry (HPLC-ESI-MS/MS). 
      N,N-dimethyl ethylenediamine (DMED) and its deuterated counterpart d(4)-DMED were 
      used to derivatize GAs extracted from plant tissue samples and GA standards 
      respectively. The both derivatives of GAs were mixed and then subjected to 
      HPLC-ESI-MS/MS analysis. The absolute quantification of GAs in plant tissues 
      could be achieved by calculating the peak area ratios of DMED labeled 
      GAs/d(4)-DMED labeled GAs. In the proposed strategy, the derivatization reaction 
      of the labeling reagents with GAs could be completed rapidly (within 5 min) with 
      high efficiency (>99%) under mild conditions. The resulting derivatives could 
      produce specific fragments in collision induced dissociation (CID), leading to 
      high selectivity in multiple-reaction monitoring (MRM) mode, thus enhanced the 
      reliability of the LC-MS/MS method. Furthermore, the limits of quantitation 
      (LOQs) of GAs were considerably decreased (2-32 folds) due to incorporating 
      easily ionized moieties into GAs, and the quantification of GAs in plant tissue 
      could be achieved without isotopically labeled GA standards. Good linearity was 
      obtained with correlation coefficients R(2) values of >0.99. The limits of 
      detection (LODs) and quantitation (LOQs) ranged from 0.02 to 0.74 pg and 0.07 to 
      2.45 pg, respectively. Eleven GAs could be successfully determined in spiked 
      sample with 72-128% recoveries and the relative standard deviations (RSDs) were 
      between 1.0% and 13.9%. Finally, the developed method was successfully applied 
      for the detection of GAs in 50mg (fresh weight) Oryza sativa leaves.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Hao, Yan-Hong
AU  - Hao YH
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Zhang, Zheng
AU  - Zhang Z
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Wang, Lu
AU  - Wang L
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Liu, Chao
AU  - Liu C
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Lei, Ai-Wen
AU  - Lei AW
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Yuan, Bi-Feng
AU  - Yuan BF
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), Department of Chemistry, Wuhan University, Wuhan 430072, PR China. 
      Electronic address: yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150624
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Ethylenediamines)
RN  - 0 (Gibberellins)
RN  - 110-70-3 (dimethylethylenediamine)
RN  - AR09D82C7G (Deuterium)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Deuterium/chemistry
MH  - Ethylenediamines/chemistry
MH  - Gibberellins/*analysis/*chemistry
MH  - Isotope Labeling
MH  - Oryza/chemistry
MH  - Plant Leaves/chemistry
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
OTO - NOTNLM
OT  - Gibberellins
OT  - HPLC-ESI-MS/MS
OT  - Stable isotope labeling
EDAT- 2015/10/11 06:00
MHDA- 2016/07/19 06:00
CRDT- 2015/10/11 06:00
PHST- 2015/04/04 00:00 [received]
PHST- 2015/06/17 00:00 [revised]
PHST- 2015/06/20 00:00 [accepted]
PHST- 2015/10/11 06:00 [entrez]
PHST- 2015/10/11 06:00 [pubmed]
PHST- 2016/07/19 06:00 [medline]
AID - S0039-9140(15)30098-9 [pii]
AID - 10.1016/j.talanta.2015.06.056 [doi]
PST - ppublish
SO  - Talanta. 2015 Nov 1;144:341-8. doi: 10.1016/j.talanta.2015.06.056. Epub 2015 Jun 
      24.