PMID- 26453270
OWN - NLM
STAT- MEDLINE
DCOM- 20160511
LR  - 20160108
IS  - 1556-5653 (Electronic)
IS  - 0015-0282 (Linking)
VI  - 105
IP  - 1
DP  - 2016 Jan
TI  - Spontaneous fertility and in vitro fertilization outcome: new evidence of human 
      papillomavirus sperm infection.
PG  - 65-72.e1
LID - S0015-0282(15)01935-4 [pii]
LID - 10.1016/j.fertnstert.2015.09.018 [doi]
AB  - OBJECTIVE: To evaluate the reproductive outcome of infertile couples undergoing 
      assisted reproduction techniques (ART) with or without human papillomavirus (HPV) 
      semen infection. DESIGN: Cross-sectional clinical study. SETTING: Units of 
      andrology, reproductive medicine, and gynecology. PATIENT(S): A total of 226 
      infertile couples. INTERVENTION(S): Male partners were evaluated by means of 
      fluorescence in situ hybridization (FISH) for HPV on semen. After a diagnostic 
      period, female partners underwent intrauterine insemination (IUI) or 
      intracytoplasmic sperm injection (ICSI). MAIN OUTCOME MEASURE(S): Seminal 
      parameters and FISH analysis for HPV in sperm head. Spontaneous or assisted 
      pregnancies, live births, and miscarriages were recorded. Statistical analysis 
      included unpaired Student t test and chi-square test. RESULT(S): Fifty-four male 
      partners (23.9%) had HPV semen infection confined to sperm, confined to 
      exfoliated cells, or in both cells. During the diagnostic period, noninfected 
      couples showed spontaneous pregnancies. IUI and ICSI treatments were performed 
      in, respectively, 60 and 98 noninfected and in 21 and 33 infected couples, with 
      38.4% and 14.2% cumulative pregnancy rates, respectively. The follow-up of 
      pregnancies showed a higher miscarriage rate in infected couples (62.5% vs. 
      16.7%). Ongoing pregnancies of the latter group were characterized by HPV 
      infection confined to exfoliated cells. CONCLUSION(S): A reduction in natural and 
      assisted cumulative pregnancy rate and an increase in miscarriage rate are 
      related to the presence of HPV at sperm level. Although the exact mechanism by 
      which sperm infection is able to impair fertility remains unclear, this aspect is 
      worthy of further investigations. If confirmed, these results could change the 
      clinical and diagnostic approach to infertile couples.
CI  - Copyright (c) 2016 American Society for Reproductive Medicine. Published by 
      Elsevier Inc. All rights reserved.
FAU - Garolla, Andrea
AU  - Garolla A
AD  - Unit of Andrology and Reproductive Medicine, Department of Medicine, University 
      of Padova, Padova, Italy. Electronic address: andrea.garolla@unipd.it.
FAU - Engl, Bruno
AU  - Engl B
AD  - Obstetrics and Gynecology Unit, Brunico, Italy.
FAU - Pizzol, Damiano
AU  - Pizzol D
AD  - Unit of Andrology and Reproductive Medicine, Department of Medicine, University 
      of Padova, Padova, Italy.
FAU - Ghezzi, Marco
AU  - Ghezzi M
AD  - Unit of Andrology and Reproductive Medicine, Department of Medicine, University 
      of Padova, Padova, Italy.
FAU - Bertoldo, Alessandro
AU  - Bertoldo A
AD  - Unit of Andrology and Reproductive Medicine, Department of Medicine, University 
      of Padova, Padova, Italy.
FAU - Bottacin, Alberto
AU  - Bottacin A
AD  - Unit of Andrology and Reproductive Medicine, Department of Medicine, University 
      of Padova, Padova, Italy.
FAU - Noventa, Marco
AU  - Noventa M
AD  - Department of Woman and Child Health, University of Padova, Padova, Italy.
FAU - Foresta, Carlo
AU  - Foresta C
AD  - Unit of Andrology and Reproductive Medicine, Department of Medicine, University 
      of Padova, Padova, Italy.
LA  - eng
PT  - Journal Article
PT  - Observational Study
DEP - 20151009
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Abortion, Spontaneous/virology
MH  - Adult
MH  - Cross-Sectional Studies
MH  - DNA, Viral/isolation & purification
MH  - Female
MH  - *Fertility
MH  - Human Papillomavirus DNA Tests
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infertility, Female/diagnosis/physiopathology/*therapy/virology
MH  - *Insemination, Artificial, Homologous
MH  - Male
MH  - Papillomaviridae/genetics/*isolation & purification
MH  - Papillomavirus Infections/complications/diagnosis/*virology
MH  - Predictive Value of Tests
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - Prospective Studies
MH  - Risk Factors
MH  - *Sperm Injections, Intracytoplasmic
MH  - Spermatozoa/*virology
MH  - Treatment Outcome
OTO - NOTNLM
OT  - HPV semen infection
OT  - IVF failure
OT  - male infertility
OT  - miscarriage
OT  - spontaneous fertility
EDAT- 2015/10/11 06:00
MHDA- 2016/05/12 06:00
CRDT- 2015/10/11 06:00
PHST- 2015/06/18 00:00 [received]
PHST- 2015/09/12 00:00 [revised]
PHST- 2015/09/14 00:00 [accepted]
PHST- 2015/10/11 06:00 [entrez]
PHST- 2015/10/11 06:00 [pubmed]
PHST- 2016/05/12 06:00 [medline]
AID - S0015-0282(15)01935-4 [pii]
AID - 10.1016/j.fertnstert.2015.09.018 [doi]
PST - ppublish
SO  - Fertil Steril. 2016 Jan;105(1):65-72.e1. doi: 10.1016/j.fertnstert.2015.09.018. 
      Epub 2015 Oct 9.