PMID- 26455231
OWN - NLM
STAT- MEDLINE
DCOM- 20160112
LR  - 20181202
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 28
IP  - 6
DP  - 2014 Jun
TI  - [IMMUNOGENICITY OF HUMAN UMBILICAL CORD BLOOD DERIVED MESENCHYMAL STEM CELLS 
      AFTER OSTEOGENIC INDUCTION].
PG  - 752-7
AB  - OBJECTIVE: To study the immunological properties of osteogenically differentiated 
      umbilical cord blood derived mesenchymal stem cells (UCB-MSCs). METHODS: UCB-MSCs 
      were isolated from the umbilical cord vein, and were expanded; the cells at 
      passage 3 were osteogenically induced for 2 weeks in vitro. The expressions of 
      human leukocyte antigen I (HLA-I) and HLA-II molecules were observed by flow 
      cytometry analysis before and after osteogenic induction. Peripheral blood T 
      lymphocytes were isolated and cultured with osteoblastic induced or 
      non-osteoblastic induced UCB-MSCs in different cell concentrations of 1 x 10(2), 
      1 x 10(3), 1 x 10(4), and 1 x 10(5) cells/well. The intake value of 3H-thymidine 
      was calculated with luminescence counter. Then T lymphocytes were pretreated with 
      PHA, and co-cultured with osteoblastic induced and non-osteoblastic induced 
      UCB-MSCs as described above. IL-2 was further added to test the reversed effect 
      of T lymphocytes proliferation stimulated by UCB-MSCs. Finally, to investigate 
      whether the immunomodulatory effects on T lymphocytes proliferation depend on 
      direct or indirect cell contact, the Transwell chamber culture system of UCB-MSCs 
      and T lymphocytes was established. RESULTS: Flow cytometry analysis showed that 
      non-osteoblastic induced UCB-MSCs expressed HLA-I but did not express HLA-II; the 
      expression of HLA-II increased in osteoblastic induced UCB-MSCs. No T lymphocyte 
      response was stimulated by non-osteoblastic induced UCB-MSCs, but osteoblastic 
      induced UCB-MSCs could stimulate the proliferation of allogeneic T lymphocytes, 
      especially after IFN-gamma treatment. Non-osteoblastic induced UCB-MSCs of 1 x 10(4) 
      and 1 x 10(5) cells/well could suppress the proliferation of T lymphocytes evoked 
      by PHA, and this suppression could be reversed by the addition of IL-2. While 
      osteoblastic induced UCB-MSCs did not have such suppressive effect. The results 
      of the Transwell culture system also showed that non-osteoblastic induced 
      UCB-MSCs could obviously inhibit the proliferation of T lymphocytes, but the 
      osteoblastic induced UCB-MSCs could not. CONCLUSION: The immunological properties 
      of UCB-MSCs will change accordingly after osteogenic induction, so UCB-MSCs might 
      not be suitable for the seed cells of bone tissue engineering.
FAU - Yang, Dawei
AU  - Yang D
FAU - Lin, Hemin
AU  - Lin H
FAU - Liu, Guangpeng
AU  - Liu G
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Coculture Techniques
MH  - Fetal Blood/cytology/*immunology
MH  - Flow Cytometry
MH  - HLA Antigens/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Umbilical Cord
EDAT- 2014/06/01 00:00
MHDA- 2016/01/13 06:00
CRDT- 2015/10/13 06:00
PHST- 2015/10/13 06:00 [entrez]
PHST- 2014/06/01 00:00 [pubmed]
PHST- 2016/01/13 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2014 Jun;28(6):752-7.