PMID- 26456329
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR  - 20220331
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 175
DP  - 2015 Dec 4
TI  - Evaluation of subacute toxicity of methanolic/aqueous preparation of aerial parts 
      of O. sanctum in Wistar rats: Clinical, haematological, biochemical and 
      histopathological studies.
PG  - 509-17
LID - S0378-8741(15)30174-4 [pii]
LID - 10.1016/j.jep.2015.10.015 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Ocimum sanctum, commonly known as Holy Basil or 
      Tulsi has been used in Ayurveda as a demulcent, stimulant, expectorant; in the 
      treatment of bronchitis, skin infections, malaria, diarrhoea, dysentery, 
      arthritis, gastric and inflammatory disorders. We have previously shown that 
      methanolic/aqueous extract of O. sanctum did not induce genotoxicity and other 
      toxic effects in acute oral toxicity study. In the present report, we have 
      performed sub-acute toxicity of methanolic/aqueous preparation of O. sanctum in 
      Wistar rats to evaluate whether it induced any chronic toxic effects. MATERIALS 
      AND METHODS: In subacute toxicity study, animals received O. sanctum extract 
      (OSE) by oral gavage at the doses of 250, 500 and 1000 mg/kg/day (n=5/group/sex) 
      for 28 days. At the end of the study, the animals were sacrificed and evaluated 
      for the effect of OSE on clinical, haematological, biochemical and 
      histopathological parameters. RESULTS: The rats treated with OSE did not show any 
      change in body weight, food and water consumption, motor activity, sensory 
      reactivity and foot splay measurements. There were no significant changes in 
      haematological, pathological and biochemical parameters; and histopathology of 
      tissues (liver, kidney, spleen, heart, and testis/ovary) among rats of either 
      sex. OSE at a dose of 1000 mg/kg showed significant increase of Mean corpuscular 
      hemoglobin (MCH) (19.8 +/- 0.8; 18.7 +/- 0.5) and mean corpuscular hemoglobin 
      concentration (MCHC) (41.8 +/- 1.1; 39.3 +/- 0.7) in male and female rats in 
      comparison to their respective controls (MCH: 17.7 +/- 0.3; 17.4 +/- 0.3; MCHC: 37.8 
      +/- 0.5; 36.1 +/- 0.2). Urine parameters (appearance, blood, nitrate, leucocyte, 
      glucose, ketone, pH, protein and specific gravity) in both the male and female 
      rats were comparable to their respective controls. In addition, no changes were 
      observed in the vital organs of rats at macroscopic and microscopic levels. 
      CONCLUSIONS: Our results showed that oral administration of OSE was not toxic to 
      male and female Wistar rats upto the highest dose tested, thereby suggesting its 
      clinical usefulness.
CI  - Copyright (c) 2015 Elsevier Ireland Ltd. All rights reserved.
FAU - Raina, P
AU  - Raina P
AD  - Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth 
      University Medical College Campus, Katraj-Dhankawadi, Pune 411043, India.
FAU - Chandrasekaran, C V
AU  - Chandrasekaran CV
AD  - Natural Remedies Pvt. Ltd., 5B Veerasandra Industrial Area, 19th KM Stone, Hosur 
      Road, Electronic City Post, Bangalore 560100, Karnataka, India.
FAU - Deepak, M
AU  - Deepak M
AD  - Natural Remedies Pvt. Ltd., 5B Veerasandra Industrial Area, 19th KM Stone, Hosur 
      Road, Electronic City Post, Bangalore 560100, Karnataka, India.
FAU - Agarwal, A
AU  - Agarwal A
AD  - Natural Remedies Pvt. Ltd., 5B Veerasandra Industrial Area, 19th KM Stone, Hosur 
      Road, Electronic City Post, Bangalore 560100, Karnataka, India.
FAU - Ruchika, K-G
AU  - Ruchika KG
AD  - Interactive Research School for Health Affairs (IRSHA), Bharati Vidyapeeth 
      University Medical College Campus, Katraj-Dhankawadi, Pune 411043, India. 
      Electronic address: ruchika.kaulghanekar@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20151009
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Plant Extracts)
RN  - 0 (Solvents)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Erythrocyte Indices/drug effects
MH  - Female
MH  - Male
MH  - Methanol/chemistry
MH  - *Ocimum
MH  - Plant Components, Aerial/chemistry
MH  - Plant Extracts/*toxicity
MH  - Rats, Wistar
MH  - Solvents/chemistry
MH  - Toxicity Tests, Subacute
OTO - NOTNLM
OT  - Methanolic/aqueous extract
OT  - O. sanctum
OT  - Subacute toxicology
EDAT- 2015/10/13 06:00
MHDA- 2016/10/07 06:00
CRDT- 2015/10/13 06:00
PHST- 2015/06/23 00:00 [received]
PHST- 2015/08/17 00:00 [revised]
PHST- 2015/10/06 00:00 [accepted]
PHST- 2015/10/13 06:00 [entrez]
PHST- 2015/10/13 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
AID - S0378-8741(15)30174-4 [pii]
AID - 10.1016/j.jep.2015.10.015 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2015 Dec 4;175:509-17. doi: 10.1016/j.jep.2015.10.015. Epub 
      2015 Oct 9.