PMID- 26457244
OWN - NLM
STAT- MEDLINE
DCOM- 20160606
LR  - 20181113
IS  - 2210-7185 (Electronic)
IS  - 2210-7177 (Print)
IS  - 2210-7177 (Linking)
VI  - 2015
DP  - 2015
TI  - Brush cytology with immunocytochemical evaluation of VEGF expression versus 
      biopsy in clinically precancerous laryngeal lesions: can we diagnose laryngeal 
      cancer only with brush cytology?
PG  - 256182
LID - 10.1155/2015/256182 [doi]
LID - 256182
AB  - Introduction. Laryngeal squamous cell carcinoma (LSCC) is the most common SCC of 
      the head and neck. The high incidence of this malignancy and the low survival 
      rate necessitate the development of novel diagnostic approaches. Aim of this 
      study is to compare the diagnostic value of laryngeal brush cytology combined 
      with VEGF immunocytochemistry versus histopathology of clinically precancerous 
      lesions of the larynx. Material and Methods. Thirty patients with precancerous or 
      suspected malignant laryngeal lesions underwent microlaryngoscopy, during which 
      samples were taken for cytological, immunocytochemical, and histological 
      analysis. Cytology and histology results were classified as follows: benign 
      lesions, atypia/moderate dysplasia, and malignancy, whereas the expression of 
      VEGF was evaluated as strong, moderate, weak, and zero expression, based on the 
      percentage of cells stained. Results. The cytology results were in accordance 
      with the histology results in 86.7% of the patients. The exfoliative cytology's 
      sensitivity was estimated at 85% and its specificity at 90%. Its positive 
      prognostic value was 94%, while its negative prognostic value was 75%. The 
      additional immunocytochemical evaluation of VEGF expression increased all the 
      noted parameters. Discussion. Exfoliative cytology of laryngeal lesions is a 
      minimal-invasive, easily applicable procedure during microlaryngoscopy and 
      reliable in terms of diagnostic value. Under certain conditions it could be held 
      also in local anesthesia. Concurrent immunocytochemical analysis of VEGF 
      expression further enhances its diagnostic value.
FAU - Chatziavramidis, Angelos
AU  - Chatziavramidis A
AD  - 2nd Academic ORL Department, Aristotle University, Papageorgiou Hospital, Ring 
      Road, Efkarpia, 56403 Thessaloniki, Greece.
FAU - Tsinaslanidou, Zinovia
AU  - Tsinaslanidou Z
AD  - 2nd Academic ORL Department, Aristotle University, Papageorgiou Hospital, Ring 
      Road, Efkarpia, 56403 Thessaloniki, Greece.
FAU - Valeri, Rozalia
AU  - Valeri R
AD  - Department of Cytopathology, "Theagenion" Oncologic Hospital, Alexandros 
      Symeonidis Street 2, 54007 Thessaloniki, Greece.
FAU - Konstantinidis, Iordanis
AU  - Konstantinidis I
AD  - 2nd Academic ORL Department, Aristotle University, Papageorgiou Hospital, Ring 
      Road, Efkarpia, 56403 Thessaloniki, Greece.
FAU - Constantinidis, Jannis
AU  - Constantinidis J
AD  - 2nd Academic ORL Department, Aristotle University, Papageorgiou Hospital, Ring 
      Road, Efkarpia, 56403 Thessaloniki, Greece.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20150917
PL  - United States
TA  - Anal Cell Pathol (Amst)
JT  - Analytical cellular pathology (Amsterdam)
JID - 101541993
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - Carcinoma, Squamous Cell/diagnosis/pathology
MH  - Cytodiagnosis/*methods
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Laryngeal Neoplasms/*diagnosis/*pathology
MH  - Male
MH  - Middle Aged
MH  - Precancerous Conditions/*diagnosis/*pathology
MH  - Vascular Endothelial Growth Factor A/*metabolism
PMC - PMC4589572
EDAT- 2015/10/13 06:00
MHDA- 2016/06/09 06:00
PMCR- 2015/09/17
CRDT- 2015/10/13 06:00
PHST- 2015/04/30 00:00 [received]
PHST- 2015/08/06 00:00 [revised]
PHST- 2015/09/02 00:00 [accepted]
PHST- 2015/10/13 06:00 [entrez]
PHST- 2015/10/13 06:00 [pubmed]
PHST- 2016/06/09 06:00 [medline]
PHST- 2015/09/17 00:00 [pmc-release]
AID - 10.1155/2015/256182 [doi]
PST - ppublish
SO  - Anal Cell Pathol (Amst). 2015;2015:256182. doi: 10.1155/2015/256182. Epub 2015 
      Sep 17.