PMID- 26457352
OWN - NLM
STAT- MEDLINE
DCOM- 20160126
LR  - 20201209
IS  - 1532-0979 (Electronic)
IS  - 0147-5185 (Linking)
VI  - 39
IP  - 11
DP  - 2015 Nov
TI  - Warthin-like Mucoepidermoid Carcinoma: A Combined Study of Fluorescence In Situ 
      Hybridization and Whole-slide Imaging.
PG  - 1479-87
LID - 10.1097/PAS.0000000000000507 [doi]
AB  - There has been some debate as to whether a subset of metaplastic Warthin tumors 
      (mWTs) harbor the mucoepidermoid carcinoma (MEC)-associated CRTC1-MAML2 fusion. 
      We analyzed 15 tumors originally diagnosed as mWT (mWT-like tumors), 2 of which 
      had concurrent MECs. We looked for the CRTC1/3-MAML2 fusion transcripts and 
      performed immunohistochemistry for p63 and fluorescence in situ hybridization 
      (FISH) for the MAML2 split. To localize MAML2 split-positive cells at the 
      cellular level, whole tumor tissue sections were digitalized (whole-slide imaging 
      [WSI]). The CRTC1-MAML2, but not CRTC3-MAML2 was detected in 5/15 mWT-like 
      tumors. FISH-WSI results showed that all epithelial cells harbored the MAML2 
      split in fusion-positive mWT-like tumors and were totally negative in 
      fusion-negative mWT-like tumors. A review of the hematoxylin and eosin-stained 
      slides showed that morphology of the "metaplastic" epithelium was virtually 
      indistinguishable between fusion-positive and fusion-negative tumors. However, 
      oncocytic bilayered tumor epithelium, characteristic to typical WT, was always 
      found somewhere in the fusion-negative tumors but not in the fusion-positive 
      tumors. This distinguishing histologic finding enabled 5 pathologists to easily 
      differentiate the 2 tumor groups with 100% accuracy. The age and sex distribution 
      of fusion-positive mWT-like tumor cases was similar to that of fusion-positive 
      MEC cases and significantly different from those of fusion-negative mWT-like 
      tumor and typical WT cases. In addition, only fusion-positive mWT-like tumors 
      possessed concurrent low-grade MECs. In conclusion, a subset of mWT-like tumors 
      were positive for the CRTC1-MAML2 fusion and had many features that are more in 
      accord with MEC than with WT. The term Warthin-like MEC should be considered for 
      fusion-positive mWT-like tumors.
FAU - Ishibashi, Kenichiro
AU  - Ishibashi K
AD  - *Department of Pathology and Molecular Diagnostics double daggerDepartment of Otolaryngology, 
      Head and Neck Surgery  section signCore Laboratory, Graduate School of Medical Sciences, 
      Nagoya City University daggerDepartment of Maxillofacial Surgery, School of Dentistry, 
      Aichi-Gakuin University, Nagoya, Japan.
FAU - Ito, Yohei
AU  - Ito Y
FAU - Masaki, Ayako
AU  - Masaki A
FAU - Fujii, Kana
AU  - Fujii K
FAU - Beppu, Shintaro
AU  - Beppu S
FAU - Sakakibara, Takeo
AU  - Sakakibara T
FAU - Takino, Hisashi
AU  - Takino H
FAU - Takase, Hiroshi
AU  - Takase H
FAU - Ijichi, Kei
AU  - Ijichi K
FAU - Shimozato, Kazuo
AU  - Shimozato K
FAU - Inagaki, Hiroshi
AU  - Inagaki H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Surg Pathol
JT  - The American journal of surgical pathology
JID - 7707904
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (CRTC1 protein, human)
RN  - 0 (CRTC3 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (MAML2 protein, human)
RN  - 0 (Nuclear Proteins)
RN  - 0 (TP63 protein, human)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Adenolymphoma/chemistry/classification/*genetics/*pathology/surgery
MH  - Biomarkers, Tumor/analysis/*genetics
MH  - Biopsy
MH  - Carcinoma, Mucoepidermoid/chemistry/classification/*genetics/*pathology/surgery
MH  - DNA-Binding Proteins/genetics
MH  - Diagnosis, Differential
MH  - Gene Fusion
MH  - Humans
MH  - Image Interpretation, Computer-Assisted
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - *Microscopy
MH  - Nuclear Proteins/genetics
MH  - Parotid Neoplasms/chemistry/classification/*genetics/*pathology/surgery
MH  - Predictive Value of Tests
MH  - Terminology as Topic
MH  - Trans-Activators
MH  - Transcription Factors/analysis/genetics
MH  - Tumor Suppressor Proteins/analysis
EDAT- 2015/10/13 06:00
MHDA- 2016/01/27 06:00
CRDT- 2015/10/13 06:00
PHST- 2015/10/13 06:00 [entrez]
PHST- 2015/10/13 06:00 [pubmed]
PHST- 2016/01/27 06:00 [medline]
AID - 00000478-201511000-00004 [pii]
AID - 10.1097/PAS.0000000000000507 [doi]
PST - ppublish
SO  - Am J Surg Pathol. 2015 Nov;39(11):1479-87. doi: 10.1097/PAS.0000000000000507.