PMID- 26458114
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR  - 20240324
IS  - 1878-0180 (Electronic)
IS  - 1751-6161 (Print)
IS  - 1878-0180 (Linking)
VI  - 54
DP  - 2016 Feb
TI  - Emulsion templated scaffolds with tunable mechanical properties for bone tissue 
      engineering.
PG  - 159-72
LID - S1751-6161(15)00356-2 [pii]
LID - 10.1016/j.jmbbm.2015.09.019 [doi]
AB  - Polymerised High Internal Phase Emulsions (PolyHIPEs) are manufactured via 
      emulsion templating and exhibit a highly interconnected microporosity. These 
      materials are commonly used as thin membranes for 3D cell culture. This study 
      uses emulsion templating in combination with microstereolithography to fabricate 
      PolyHIPE scaffolds with a tightly controlled and reproducible architecture. This 
      combination of methods produces hierarchical structures, where the 
      microstructural properties can be independently controlled from the scaffold 
      macrostructure. PolyHIPEs were fabricated with varying ratios of two acrylate 
      monomers (2-ethylhexyl acrylate (EHA) and isobornyl acrylate (IBOA)) and varying 
      nominal porosity to tune mechanical properties. Young's modulus, ultimate tensile 
      stress (UTS) and elongation at failure were determined for twenty EHA/IBOA 
      compositions. Moduli ranged from 63.01+/-9.13 to 0.36+/-0.04MPa, UTS from 2.03+/-0.33 
      to 0.11+/-0.01MPa and failure strain from 21.86+/-2.87% to 2.60+/-0.61%. Selected 
      compositions were fabricated into macro-porous woodpile structures, plasma 
      treated with air or acrylic acid and seeded with human embryonic stem-cell 
      derived mesenchymal progenitor cells (hES-MPs). Confocal and two-photon 
      microscopy confirmed cell proliferation and penetration into the micro- and 
      macro-porous architecture. The scaffolds supported osteogenic differentiation of 
      mesenchymal cells and interestingly, the stiffest IBOA-based scaffolds that were 
      plasma treated with acrylic acid promoted osteogenesis more strongly than the 
      other scaffolds.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Owen, Robert
AU  - Owen R
AD  - Department of Materials Science and Engineering, University of Sheffield, 
      INSIGNEO Institute for in silico medicine, The Pam Liversidge Building, Sir 
      Frederick Mappin Building, Mappin Street, Sheffield S1 3JD, United Kingdom.
FAU - Sherborne, Colin
AU  - Sherborne C
AD  - Department of Materials Science and Engineering, University of Sheffield, The 
      Kroto Research Institute, North Campus, Broad Lane, Sheffield S3 7HQ, United 
      Kingdom.
FAU - Paterson, Thomas
AU  - Paterson T
AD  - Department of Materials Science and Engineering, University of Sheffield, The 
      Kroto Research Institute, North Campus, Broad Lane, Sheffield S3 7HQ, United 
      Kingdom.
FAU - Green, Nicola H
AU  - Green NH
AD  - Department of Materials Science and Engineering, University of Sheffield, The 
      Kroto Research Institute, North Campus, Broad Lane, Sheffield S3 7HQ, United 
      Kingdom.
FAU - Reilly, Gwendolen C
AU  - Reilly GC
AD  - Department of Materials Science and Engineering, University of Sheffield, 
      INSIGNEO Institute for in silico medicine, The Pam Liversidge Building, Sir 
      Frederick Mappin Building, Mappin Street, Sheffield S1 3JD, United Kingdom.
FAU - Claeyssens, Frederik
AU  - Claeyssens F
AD  - Department of Materials Science and Engineering, University of Sheffield, The 
      Kroto Research Institute, North Campus, Broad Lane, Sheffield S3 7HQ, United 
      Kingdom. Electronic address: f.claeyssens@sheffield.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150925
PL  - Netherlands
TA  - J Mech Behav Biomed Mater
JT  - Journal of the mechanical behavior of biomedical materials
JID - 101322406
RN  - 0 (Biocompatible Materials)
RN  - 0 (Emulsions)
SB  - IM
MH  - Biocompatible Materials/*chemistry/*pharmacology
MH  - Bone and Bones/*cytology
MH  - Cell Adhesion/drug effects
MH  - Cell Differentiation/drug effects
MH  - Embryonic Stem Cells/cytology
MH  - Emulsions
MH  - Humans
MH  - *Mechanical Phenomena
MH  - Mesenchymal Stem Cells/cytology/drug effects
MH  - Osteogenesis/drug effects
MH  - Porosity
MH  - Tensile Strength
MH  - *Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
PMC - PMC4717122
OTO - NOTNLM
OT  - Free form fabrication
OT  - Mechanical properties
OT  - Plasma polymerization
OT  - PolyHIPEs
OT  - Porosity
OT  - Stereolithography
EDAT- 2015/10/13 06:00
MHDA- 2016/10/07 06:00
PMCR- 2016/02/01
CRDT- 2015/10/13 06:00
PHST- 2015/04/22 00:00 [received]
PHST- 2015/09/09 00:00 [revised]
PHST- 2015/09/14 00:00 [accepted]
PHST- 2015/10/13 06:00 [entrez]
PHST- 2015/10/13 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
PHST- 2016/02/01 00:00 [pmc-release]
AID - S1751-6161(15)00356-2 [pii]
AID - 10.1016/j.jmbbm.2015.09.019 [doi]
PST - ppublish
SO  - J Mech Behav Biomed Mater. 2016 Feb;54:159-72. doi: 10.1016/j.jmbbm.2015.09.019. 
      Epub 2015 Sep 25.