PMID- 26460099
OWN - NLM
STAT- MEDLINE
DCOM- 20160706
LR  - 20220408
IS  - 1552-3365 (Electronic)
IS  - 0363-5465 (Linking)
VI  - 43
IP  - 12
DP  - 2015 Dec
TI  - Leukocyte-Reduced Platelet-Rich Plasma Normalizes Matrix Metabolism in Torn Human 
      Rotator Cuff Tendons.
PG  - 2898-906
LID - 10.1177/0363546515608157 [doi]
AB  - BACKGROUND: The optimal platelet-rich plasma (PRP) for treatment of supraspinatus 
      tendinopathy has not been determined. PURPOSE: To evaluate the effect of low- 
      versus high-leukocyte concentrated PRP products on catabolic and anabolic 
      mediators of matrix metabolism in diseased rotator cuff tendons. STUDY DESIGN: 
      Controlled laboratory study. METHODS: Diseased supraspinatus tendons were treated 
      with PRP made by use of 2 commercial systems: Arthrex Autologous Conditioned 
      Plasma Double Syringe System (L(lo) PRP) and Biomet GPS III Mini Platelet 
      Concentrate System (L(hi) PRP). Tendon explants were placed in 6-well plates and 
      cultured in L(lo) PRP, L(hi) PRP, or control media (Dulbecco's Modified Eagle 
      Medium + 10% fetal bovine serum) for 96 hours. Tendons were processed for 
      hematoxylin-eosin histologic results and were scored with the modified Bonar 
      scale. Group 1 tendons were defined as moderate tendinopathy (Bonar score <3); 
      group 2 tendons were assessed as severely affected (Bonar score = 3). 
      Transforming growth factor beta-1 (TGFbeta-1), interleukin-1beta (IL-1beta), interleukin-1 
      receptor antagonist (IL-1Ra), interleukin-6 (IL-6), interleukin-8 (IL-8), and 
      matrix metalloproteinase-9 (MMP-9) concentrations in PRP media were measured by 
      use of enzyme-linked immunosorbent assay after 96 hours of culture with diseased 
      tendon. Tendon messenger RNA expression of collagen type I (COL1A1), collagen 
      type III (COL3A1), cartilage oligomeric matrix protein (COMP), MMP-9, MMP-13, and 
      IL-1beta was measured with real-time quantitative polymerase chain reaction. 
      RESULTS: Leukocytes and platelets were significantly more concentrated in L(hi) 
      PRP compared with L(lo) PRP. Increased IL-1beta was present in L(hi) PRP after 
      culture with group 1 tendons. IL-6 was increased in L(hi) PRP after culture with 
      group 2 tendons. Both TGFbeta-1 and MMP-9 were increased in L(hi) PRP after culture 
      with either tendon group. In L(lo) PRP cultures, IL-1Ra:IL-1beta in PRP used as 
      media and COL1A1:COL3A1 gene expression were increased for group 1 tendon 
      cultures. Gene expression of MMP-9 and IL-1beta was increased in group 2 tendons 
      cultured in L(lo) PRP. There was no significant difference in the expression of 
      MMP-13 or COMP in either group of tendons cultured in L(lo) PRP or L(hi) PRP. 
      CONCLUSION: L(lo) PRP promotes normal collagen matrix synthesis and decreases 
      cytokines associated with matrix degradation and inflammation to a greater extent 
      than does L(hi) PRP in moderately degenerative tendons. In severely degenerative 
      tendons, neither PRP preparation enhanced matrix synthesis. CLINICAL RELEVANCE: 
      L(lo) PRP may promote healing in moderately degenerative rotator cuff tendons.
CI  - (c) 2015 The Author(s).
FAU - Cross, Jessica A
AU  - Cross JA
AD  - Department of Clinical Sciences, Cornell University, Ithaca, New York, USA.
FAU - Cole, Brian J
AU  - Cole BJ
AD  - Department of Orthopedics, Rush University Medical Center, Chicago, Illinois, USA 
      cole.research@rushortho.com.
FAU - Spatny, Kaylan P
AU  - Spatny KP
AD  - Department of Clinical Sciences, Cornell University, Ithaca, New York, USA.
FAU - Sundman, Emily
AU  - Sundman E
AD  - Department of Clinical Sciences, Cornell University, Ithaca, New York, USA.
FAU - Romeo, Anthony A
AU  - Romeo AA
AD  - Department of Orthopedics, Rush University Medical Center, Chicago, Illinois, 
      USA.
FAU - Nicholson, Greg P
AU  - Nicholson GP
AD  - Department of Orthopedics, Rush University Medical Center, Chicago, Illinois, 
      USA.
FAU - Wagner, Bettina
AU  - Wagner B
AD  - Department of Population Medicine and Diagnostic Sciences, Cornell University, 
      Ithaca, New York, USA.
FAU - Fortier, Lisa A
AU  - Fortier LA
AD  - Department of Clinical Sciences, Cornell University, Ithaca, New York, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151012
PL  - United States
TA  - Am J Sports Med
JT  - The American journal of sports medicine
JID - 7609541
RN  - 0 (Cytokines)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Proto-Oncogene Proteins c-sis)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Cytokines/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Humans
MH  - Platelet-Rich Plasma/*metabolism
MH  - Proto-Oncogene Proteins c-sis/metabolism
MH  - Tendinopathy/*metabolism/therapy
MH  - Transforming Growth Factor beta1/metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - biological healing enhancement
OT  - growth factors
OT  - platelet-rich plasma
OT  - rotator cuff
OT  - shoulder
EDAT- 2015/10/16 06:00
MHDA- 2016/07/07 06:00
CRDT- 2015/10/14 06:00
PHST- 2015/10/14 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/07/07 06:00 [medline]
AID - 0363546515608157 [pii]
AID - 10.1177/0363546515608157 [doi]
PST - ppublish
SO  - Am J Sports Med. 2015 Dec;43(12):2898-906. doi: 10.1177/0363546515608157. Epub 
      2015 Oct 12.