PMID- 26460687
OWN - NLM
STAT- MEDLINE
DCOM- 20160606
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 10
DP  - 2015
TI  - Expression of CD1a and Type-1 Polarization Are Dissociated in Human 
      Monocyte-Derived Dendritic Cells.
PG  - e0140432
LID - 10.1371/journal.pone.0140432 [doi]
LID - e0140432
AB  - Ex vivo generated monocyte-derived dendritic cell (moDC)-vaccines have long been 
      touted as promising immunotherapeutic agents for cancer treatment, although the 
      response rate generally remains low. The reasons for this are still unclear and 
      confounded by the diversity in manufacturing protocols that may affect moDC 
      function. Preclinical studies have shown that the stimulatory function of 
      dendritic cells can be improved by engaging invariant NKT cells in vivo through 
      the presentation of the glycolipid alpha-galactosylceramide via CD1d. However, 
      expression of CD1d on moDC has been shown to be negatively correlated with 
      expression of CD1a, which in turn has been suggested to be a surrogate marker for 
      IL-12 secreting type-1 polarized moDC, the preferred functional characteristics 
      for cancer vaccines. Here we challenge this notion by showing that plasma-derived 
      lipids drive functional levels of CD1d expression, while CD1a expression can vary 
      considerably in these cells without being correlated with a loss of polarization 
      or immunogenicity.
FAU - Mester, Brigitta
AU  - Mester B
AD  - Malaghan Institute of Medical Research, Wellington, New Zealand.
FAU - Bauer, Evelyn
AU  - Bauer E
AD  - Malaghan Institute of Medical Research, Wellington, New Zealand.
FAU - Wood, Catherine E
AU  - Wood CE
AD  - Malaghan Institute of Medical Research, Wellington, New Zealand; Capital and 
      Coast District Health Board, Wellington, New Zealand.
FAU - Hermans, Ian F
AU  - Hermans IF
AD  - Malaghan Institute of Medical Research, Wellington, New Zealand; School of 
      Biological Sciences, Victoria University of Wellington, Wellington, New Zealand; 
      Maurice Wilkins Centre, Wellington, New Zealand.
FAU - Gasser, Olivier
AU  - Gasser O
AD  - Malaghan Institute of Medical Research, Wellington, New Zealand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151013
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1a antigen)
SB  - IM
MH  - Antigens, CD1/*metabolism
MH  - *Cell Polarity
MH  - Dendritic Cells/*cytology/*metabolism
MH  - Humans
MH  - Monocytes/*cytology
MH  - Phenotype
MH  - Plasma
PMC - PMC4603902
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/10/16 06:00
MHDA- 2016/06/09 06:00
PMCR- 2015/10/13
CRDT- 2015/10/14 06:00
PHST- 2015/07/27 00:00 [received]
PHST- 2015/09/25 00:00 [accepted]
PHST- 2015/10/14 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/06/09 06:00 [medline]
PHST- 2015/10/13 00:00 [pmc-release]
AID - PONE-D-15-32836 [pii]
AID - 10.1371/journal.pone.0140432 [doi]
PST - epublish
SO  - PLoS One. 2015 Oct 13;10(10):e0140432. doi: 10.1371/journal.pone.0140432. 
      eCollection 2015.