PMID- 26464217
OWN - NLM
STAT- MEDLINE
DCOM- 20160527
LR  - 20240210
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 46
IP  - 1
DP  - 2016 Jan
TI  - Depletion of CD11c(+) cells in the CD11c.DTR model drives expansion of unique CD64(+) 
      Ly6C(+) monocytes that are poised to release TNF-alpha.
PG  - 192-203
LID - 10.1002/eji.201545789 [doi]
AB  - Dendritic cells (DCs) play a vital role in innate and adaptive immunities. 
      Inducible depletion of CD11c(+) DCs engineered to express a high-affinity 
      diphtheria toxin receptor has been a powerful tool to dissect DC function in 
      vivo. However, despite reports showing that loss of DCs induces transient 
      monocytosis, the monocyte population that emerges and the potential impact of 
      monocytes on studies of DC function have not been investigated. We found that 
      depletion of CD11c(+) cells from CD11c.DTR mice induced the expansion of a 
      variant CD64(+) Ly6C(+) monocyte population in the spleen and blood that was 
      distinct from conventional monocytes. Expansion of CD64(+) Ly6C(+) monocytes was 
      independent of mobilization from the BM via CCR2 but required the cytokine, 
      G-CSF. Indeed, this population was also expanded upon exposure to exogenous G-CSF 
      in the absence of DC depletion. CD64(+) Ly6C(+) monocytes were characterized by 
      upregulation of innate signaling apparatus despite the absence of inflammation, 
      and an increased capacity to produce TNF-alpha following LPS stimulation. Thus, 
      depletion of CD11c(+) cells induces expansion of a unique CD64(+) Ly6C(+) 
      monocyte population poised to synthesize TNF-alpha. This finding will require 
      consideration in experiments using depletion strategies to test the role of 
      CD11c(+) DCs in immunity.
CI  - (c) 2015 The Authors. European Journal of Immunology published by WILEY-VCH Verlag 
      GmbH & Co. KGaA, Weinheim.
FAU - Sivakumaran, Shivajanani
AU  - Sivakumaran S
AD  - Institute for Immunity and Transplantation, University College London, London, 
      UK.
AD  - Cancer Institute, University College London, London, UK.
FAU - Henderson, Stephen
AU  - Henderson S
AD  - Cancer Institute, University College London, London, UK.
AD  - Bill Lyons Informatics Centre, University College London, London, UK.
FAU - Ward, Sophie
AU  - Ward S
AD  - Institute for Immunity and Transplantation, University College London, London, 
      UK.
AD  - Cancer Institute, University College London, London, UK.
FAU - Sousa, Pedro Santos E
AU  - Sousa PS
AD  - Institute for Immunity and Transplantation, University College London, London, 
      UK.
AD  - Cancer Institute, University College London, London, UK.
FAU - Manzo, Teresa
AU  - Manzo T
AD  - Institute for Immunity and Transplantation, University College London, London, 
      UK.
AD  - Cancer Institute, University College London, London, UK.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Institute for Immunity and Transplantation, University College London, London, 
      UK.
AD  - Cancer Institute, University College London, London, UK.
FAU - Conlan, Thomas
AU  - Conlan T
AD  - Institute for Immunity and Transplantation, University College London, London, 
      UK.
AD  - Cancer Institute, University College London, London, UK.
FAU - Means, Terry K
AU  - Means TK
AD  - MGH Center for Immunology and Inflammatory Diseases, Harvard Medical School, 
      Boston, MA, USA.
FAU - D'Aveni, Maud
AU  - D'Aveni M
AD  - CNRS UMR 8147, Universite Paris Descartes, Faculte de Medecine, Hopital Necker, 
      Paris, France.
FAU - Hermine, Olivier
AU  - Hermine O
AD  - CNRS UMR 8147, Universite Paris Descartes, Faculte de Medecine, Hopital Necker, 
      Paris, France.
FAU - Rubio, Marie-Therese
AU  - Rubio MT
AD  - CNRS UMR 8147, Universite Paris Descartes, Faculte de Medecine, Hopital Necker, 
      Paris, France.
FAU - Chakraverty, Ronjon
AU  - Chakraverty R
AD  - Institute for Immunity and Transplantation, University College London, London, 
      UK.
AD  - Cancer Institute, University College London, London, UK.
FAU - Bennett, Clare L
AU  - Bennett CL
AD  - Institute for Immunity and Transplantation, University College London, London, 
      UK.
AD  - Cancer Institute, University College London, London, UK.
LA  - eng
GR  - 20265/CRUK_/Cancer Research UK/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151130
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, Ly)
RN  - 0 (CD11c Antigen)
RN  - 0 (Ly-6C antigen, mouse)
RN  - 0 (Receptors, IgG)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Antigens, Ly/immunology
MH  - CD11c Antigen/immunology
MH  - Dendritic Cells/*immunology
MH  - Flow Cytometry
MH  - Mice
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Monocytes/*cytology/*immunology
MH  - Oligonucleotide Array Sequence Analysis
MH  - Receptors, IgG/immunology
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/immunology
PMC - PMC4722854
OTO - NOTNLM
OT  - CD11c.DTR
OT  - Dendritic cells
OT  - Depletion
OT  - Diphtheria toxin receptor (DTR)
OT  - Monocytes
OT  - Monocytosis
EDAT- 2015/10/16 06:00
MHDA- 2016/05/28 06:00
PMCR- 2016/01/22
CRDT- 2015/10/15 06:00
PHST- 2015/05/07 00:00 [received]
PHST- 2015/09/10 00:00 [revised]
PHST- 2015/10/07 00:00 [accepted]
PHST- 2015/10/15 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/05/28 06:00 [medline]
PHST- 2016/01/22 00:00 [pmc-release]
AID - 10.1002/eji.201545789 [doi]
PST - ppublish
SO  - Eur J Immunol. 2016 Jan;46(1):192-203. doi: 10.1002/eji.201545789. Epub 2015 Nov 
      30.