PMID- 26464679 OWN - NLM STAT- MEDLINE DCOM- 20160803 LR - 20181113 IS - 1936-2625 (Electronic) IS - 1936-2625 (Linking) VI - 8 IP - 8 DP - 2015 TI - Tumor necrosis factor-alpha gene 308G/A polymorphism is not associated with esophageal squamous cell carcinoma risk in Kazakh patients. PG - 9293-9 AB - BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is a malignant tumor with a strong tendency toward familial aggregation and a higher incidence as well as mortality in Kazakh population. Tumor necrosis factor-alpha (TNF-alpha) is an important inflammatory cytokine that plays a role in controlling the progression of lung cancer, hepatocellular cancer, breast cancer and gastric cancer. But the association between TNF-alpha-308G/A and ESCC still remains unclarified. MATERIALS AND METHODS: Here, we investigated the potential associations between the TNF-alpha-308G/A and susceptibility to ESCC in 212 cases and 200 controls from a pure ethnic population of Kazakh. DNA extraction and Real-time PCR were performed to detect the TNF-alpha-308G/A expression levels and odd ratios (ORs) with the corresponding 95% confidence interval (CI) were to evaluate their association with TNF-alpha-308G/A polymorphism. RESULTS: We found that the frequencies of TNF-alpha-308G/A in the cases were similar to that of the controls with no differences being statistically significant (chi(2)=1.23, P>0.05). Using the G allele as the reference genotype, individuals who carried A allele had a significantly increased risk of developing ESCC (OR=2.64, 95% CI=1.31~5.35). Especially, the G/A+A/A genotype are associated with increased risk of metastatic as compared with GG genotype individuals (OR=2.08, 95% CI=1.14-3.80, P=0.02). CONCLUSIONS: Our findings suggest that though the TNF-alpha-308G/A polymorphism may not be correlated with the susceptibility to Kazakh's ESCC in Xinjiang, patients who carry A allele tend to poorly differentiated and lymph node metastasis. FAU - Cui, Xiao-Bin AU - Cui XB AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China ; Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology Wuhan 430030, China. FAU - Wang, Dan-Dan AU - Wang DD AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Zhang, Hai-Yang AU - Zhang HY AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Li, Ting-Ting AU - Li TT AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Jin, Ting-Ting AU - Jin TT AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Peng, Hao AU - Peng H AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Zhang, Shu-Mao AU - Zhang SM AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Wang, Bin AU - Wang B AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Yu, Jie AU - Yu J AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Liu, Chun-Xia AU - Liu CX AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Yang, Lan AU - Yang L AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Jin, Jing AU - Jin J AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Li, Su AU - Li S AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Jiang, Jin-Fang AU - Jiang JF AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Liang, Wei-Hua AU - Liang WH AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Hu, Jian-Ming AU - Hu JM AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Li, Shu-Gang AU - Li SG AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Wu, Chuan-Yue AU - Wu CY AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China ; Department of Pathology, University of Pittsburgh Pittsburgh, PA 15261, USA. FAU - Chen, Yun-Zhao AU - Chen YZ AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China. FAU - Li, Feng AU - Li F AD - Department of Pathology and Key Laboratory for Xinjiang Endemic and Ethnic Diseases, Shihezi University School of Medicine Shihezi 832002, China ; Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology Wuhan 430030, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20150801 PL - United States TA - Int J Clin Exp Pathol JT - International journal of clinical and experimental pathology JID - 101480565 RN - 0 (Tumor Necrosis Factor-alpha) SB - IM MH - Adult MH - Aged MH - Alleles MH - Carcinoma, Squamous Cell/*genetics/pathology MH - Case-Control Studies MH - Esophageal Neoplasms/*genetics/pathology MH - Female MH - Genetic Association Studies MH - *Genetic Predisposition to Disease MH - Genotype MH - Humans MH - Kazakhstan MH - Male MH - Middle Aged MH - *Polymorphism, Single Nucleotide MH - Tumor Necrosis Factor-alpha/*genetics MH - Young Adult PMC - PMC4583911 OTO - NOTNLM OT - Kazakh OT - Tumor necrosis factor OT - esophageal cancer OT - gene polymorphism EDAT- 2015/10/16 06:00 MHDA- 2016/08/04 06:00 PMCR- 2015/08/01 CRDT- 2015/10/15 06:00 PHST- 2015/05/31 00:00 [received] PHST- 2015/07/20 00:00 [accepted] PHST- 2015/10/15 06:00 [entrez] PHST- 2015/10/16 06:00 [pubmed] PHST- 2016/08/04 06:00 [medline] PHST- 2015/08/01 00:00 [pmc-release] PST - epublish SO - Int J Clin Exp Pathol. 2015 Aug 1;8(8):9293-9. eCollection 2015.