PMID- 26465008
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151014
LR  - 20181113
IS  - 2373-2822 (Print)
IS  - 2373-2822 (Electronic)
IS  - 2373-2822 (Linking)
VI  - 2
IP  - 4
DP  - 2015 Jul-Aug
TI  - Estradiol Preferentially Induces Progestin Receptor-A (PR-A) Over PR-B in Cells 
      Expressing Nuclear Receptor Coactivators in the Female Mouse Hypothalamus.
LID - 10.1523/ENEURO.0012-15.2015 [doi]
LID - ENEURO.0012-15.2015
AB  - Estrogens act in brain to profoundly influence neurogenesis, sexual 
      differentiation, neuroprotection, cognition, energy homeostasis, and female 
      reproductive behavior and physiology through a variety of mechanisms, including 
      the induction of progestin receptors (PRs). PRs are expressed as two isoforms, 
      PR-A and PR-B, that have distinct functions in physiology and behavior. Because 
      these PR isoforms cannot be distinguished using cellular resolution techniques, 
      the present study used isoform-specific null mutant mice that lack PR-A or PR-B 
      for the first time to investigate whether 17beta-estradiol benzoate (EB) regulates 
      the differential expression of the PR isoforms in the ventromedial nucleus of the 
      hypothalamus (VMN), arcuate nucleus, and medial preoptic area, brain regions that 
      are rich in EB-induced PRs. Interestingly, EB induced more PR-A than PR-B in all 
      three brain regions, suggesting that PR-A is the predominant isoform in these 
      regions. Given that steroid receptor coactivator (SRC)-1 and SRC-2 are important 
      in estrogen receptor (ER)-dependent transcription in brain, including PR 
      induction, we tested whether the expression of these coactivators was correlated 
      with PR isoform expression. The majority of EB-induced PR cells expressed both 
      SRC-1 and SRC-2 in the three brain regions of all genotypes. Interestingly, the 
      intensity of PR-A immunoreactivity correlated with SRC-2 expression in the VMN, 
      providing a potential mechanism for selective ER-mediated transactivation of PR-A 
      over PR-B in a brain region-specific manner. In summary, these novel findings 
      indicate that estrogens differentially regulate PR-A and PR-B expression in the 
      female hypothalamus, and provide a mechanism by which steroid action in brain can 
      selectively modulate behavior and physiology.
FAU - Acharya, Kalpana D
AU  - Acharya KD
AD  - Neuroscience Program, Wellesley College , Wellesley, Massachusetts 02481.
FAU - Finkelstein, Sarah D
AU  - Finkelstein SD
AD  - Neuroscience Program, Wellesley College , Wellesley, Massachusetts 02481.
FAU - Bless, Elizabeth P
AU  - Bless EP
AD  - Neuroscience Program, Wellesley College , Wellesley, Massachusetts 02481.
FAU - Nettles, Sabin A
AU  - Nettles SA
AD  - Neuroscience Program, Wellesley College , Wellesley, Massachusetts 02481.
FAU - Mulac-Jericevic, Biserka
AU  - Mulac-Jericevic B
AD  - Department of Molecular and Cellular Biology, Baylor College of Medicine , 
      Houston, Texas 77030.
FAU - Conneely, Orla M
AU  - Conneely OM
AD  - Department of Molecular and Cellular Biology, Baylor College of Medicine , 
      Houston, Texas 77030.
FAU - Mani, Shaila K
AU  - Mani SK
AD  - Department of Molecular and Cellular Biology, Baylor College of Medicine , 
      Houston, Texas 77030.
FAU - Tetel, Marc J
AU  - Tetel MJ
AD  - Neuroscience Program, Wellesley College , Wellesley, Massachusetts 02481.
LA  - eng
GR  - R01 DK061935/DK/NIDDK NIH HHS/United States
PT  - Journal Article
DEP - 20150813
PL  - United States
TA  - eNeuro
JT  - eNeuro
JID - 101647362
PMC - PMC4596027
OTO - NOTNLM
OT  - SRC-1
OT  - estrogens
OT  - female reproduction
OT  - progesterone
OT  - steroid receptor coactivator
OT  - ventromedial hypothalamus
COIS- The authors declare no conflicting financial interests.
EDAT- 2015/10/16 06:00
MHDA- 2015/10/16 06:01
PMCR- 2015/08/13
CRDT- 2015/10/15 06:00
PHST- 2015/01/30 00:00 [received]
PHST- 2015/07/24 00:00 [revised]
PHST- 2015/07/25 00:00 [accepted]
PHST- 2015/10/15 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2015/10/16 06:01 [medline]
PHST- 2015/08/13 00:00 [pmc-release]
AID - eN-NWR-0012-15 [pii]
AID - 10.1523/ENEURO.0012-15.2015 [doi]
PST - epublish
SO  - eNeuro. 2015 Aug 13;2(4):ENEURO.0012-15.2015. doi: 10.1523/ENEURO.0012-15.2015. 
      eCollection 2015 Jul-Aug.