PMID- 26465140
OWN - NLM
STAT- MEDLINE
DCOM- 20160531
LR  - 20190222
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 10
DP  - 2015
TI  - Development and Molecular Cytogenetic Identification of a Novel Wheat-Leymus 
      mollis Lm#7Ns (7D) Disomic Substitution Line with Stripe Rust Resistance.
PG  - e0140227
LID - 10.1371/journal.pone.0140227 [doi]
LID - e0140227
AB  - Leymus mollis (2n = 4x = 28, NsNsXmXm) possesses novel and important genes for 
      resistance against multi-fungal diseases. The development of new wheat-L. mollis 
      introgression lines is of great significance for wheat disease resistance 
      breeding. M11003-3-1-15-8, a novel disomic substitution line of common wheat cv. 
      7182 -L. mollis, developed and selected from the BC1F5 progeny between wheat cv. 
      7182 and octoploid Tritileymus M47 (2n = 8x = 56, AABBDDNsNs), was characterized 
      by morphological and cytogenetic identification, analysis of functional molecular 
      markers, genomic in situ hybridization (GISH), sequential fluorescence in situ 
      hybridization (FISH)-genomic in situ hybridization (GISH) and disease resistance 
      evaluation. Cytological observations suggested that M11003-3-1-15-8 contained 42 
      chromosomes and formed 21 bivalents at meiotic metaphase I. The GISH 
      investigations showed that line contained 40 wheat chromosomes and a pair of L. 
      mollis chromosomes. EST-STS multiple loci markers and PLUG (PCR-based Landmark 
      Unique Gene) markers confirmed that the introduced L. mollis chromosomes belonged 
      to homoeologous group 7, it was designated as Lm#7Ns. While nulli-tetrasomic and 
      sequential FISH-GISH analysis using the oligonucleotide Oligo-pSc119.2 and 
      Oligo-pTa535 as probes revealed that the wheat 7D chromosomes were absent in 
      M11003-3-1-15-8. Therefore, it was deduced that M11003-3-1-15-8 was a wheat-L. 
      mollis Lm#7Ns (7D) disomic substitution line. Field disease resistance 
      demonstrated that the introduced L. mollis chromosomes Lm#7Ns were responsible 
      for the stripe rust resistance at the adult stage. Moreover, M11003-3-1-15-8 had 
      a superior numbers of florets. The novel disomic substitution line 
      M11003-3-1-15-8, could be exploited as an important genetic material in wheat 
      resistance breeding programs and genetic resources.
FAU - Yang, Xiaofei
AU  - Yang X
AD  - College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, 
      Northwest A & F University, Yangling, Shaanxi, 712100, China.
FAU - Wang, Changyou
AU  - Wang C
AD  - College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, 
      Northwest A & F University, Yangling, Shaanxi, 712100, China.
FAU - Li, Xin
AU  - Li X
AD  - College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, 
      Northwest A & F University, Yangling, Shaanxi, 712100, China.
FAU - Chen, Chunhuan
AU  - Chen C
AD  - College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, 
      Northwest A & F University, Yangling, Shaanxi, 712100, China.
FAU - Tian, Zengrong
AU  - Tian Z
AD  - College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, 
      Northwest A & F University, Yangling, Shaanxi, 712100, China.
FAU - Wang, Yajuan
AU  - Wang Y
AD  - College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, 
      Northwest A & F University, Yangling, Shaanxi, 712100, China.
FAU - Ji, Wanquan
AU  - Ji W
AD  - College of Agronomy, State Key Laboratory of Crop Stress Biology for Arid Areas, 
      Northwest A & F University, Yangling, Shaanxi, 712100, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151014
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Basidiomycota/pathogenicity
MH  - Chromosomes, Plant/*genetics
MH  - Disease Resistance/*genetics
MH  - Genetic Markers
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Diseases/*genetics/microbiology
MH  - Plants, Genetically Modified
MH  - Poaceae/genetics
MH  - Triticum/*genetics
PMC - PMC4605682
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/10/16 06:00
MHDA- 2016/06/01 06:00
PMCR- 2015/10/14
CRDT- 2015/10/15 06:00
PHST- 2015/07/06 00:00 [received]
PHST- 2015/09/23 00:00 [accepted]
PHST- 2015/10/15 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/06/01 06:00 [medline]
PHST- 2015/10/14 00:00 [pmc-release]
AID - PONE-D-15-26639 [pii]
AID - 10.1371/journal.pone.0140227 [doi]
PST - epublish
SO  - PLoS One. 2015 Oct 14;10(10):e0140227. doi: 10.1371/journal.pone.0140227. 
      eCollection 2015.