PMID- 26466964
OWN - NLM
STAT- MEDLINE
DCOM- 20160426
LR  - 20200315
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 48
IP  - 6
DP  - 2015 Dec
TI  - HGF and IGF-1 promote protective effects of allogeneic BMSC transplantation in 
      rabbit model of acute myocardial infarction.
PG  - 661-70
LID - 10.1111/cpr.12219 [doi]
AB  - OBJECTIVES: To explore effects of hepatocyte growth factor (HGF) combined with 
      insulin-like growth factor 1 (IGF-1) on transplanted bone marrow mesenchymal stem 
      cells (BMSCs), for treatment of acute myocardial ischaemia. MATERIALS AND 
      METHODS: After ligation of the left anterior descending artery, rabbits were 
      divided into a Control group, a Factors group (HGF+IGF-1), a BMSC group and a 
      Factors+BMSCs group. Allogenous BMSCs (1 x 10(7)) and/or control-released 
      microspheres of 2 mug HGF+2 mug IGF-1 were intramyocardially injected into 
      infarcted regions. Apoptosis and differentiation of implanted BMSCs, histological 
      and morphological results, and cardiac remodelling and function were evaluated at 
      different time points. In vitro, BMSCs were exposed to HGF, IGF-1 and both (50 
      ng/ml) and subsequently proliferation, migration, myocardial differentiation and 
      apoptosis induced by hypoxia, were analysed. RESULTS: Four weeks 
      post-operatively, the above indices were significantly improved in Factors+BMSCs 
      group compared to the others (P < 0.01), although Factors and BMSCs group also 
      showed better results than Control group (P < 0.05). In vitro, HGF promoted BMSC 
      migration and differentiation into cardiomyocytes, but inhibited proliferation (P 
      < 0.05), while IGF-1 increased proliferation and migration, and inhibited 
      apoptosis induced by hypoxia (P < 0.05), but did not induce myocardial 
      differentiation. Combination of HGF and IGF-1 significantly promoted BMSCs 
      capacity for migration, differentiation and lack of apoptosis (P < 0.05). 
      CONCLUSIONS: Combination of HGF and IGF-1 activated BMSCs complementarily, and 
      controlled release of the two factors promoted protective potential of 
      transplanted BMSCs to repair infarcted myocardium. This suggests a new strategy 
      for cell therapies to overcome acute ischemic myocardial injury.
CI  - (c) 2015 John Wiley & Sons Ltd.
FAU - Zhang, Guang-Wei
AU  - Zhang GW
AD  - Department of Cardiac Surgery, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
FAU - Gu, Tian-Xiang
AU  - Gu TX
AD  - Department of Cardiac Surgery, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
AD  - The Cardiovascular Research Center of China Medical University, Shenyang, 110001, 
      China.
FAU - Guan, Xiao-Yu
AU  - Guan XY
AD  - Department of Cardiac Surgery, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
FAU - Sun, Xue-Jun
AU  - Sun XJ
AD  - Department of Anesthesiology, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
AD  - Department of Anesthesiology of the First Affiliated Hospital of Dalian Medical 
      University, Dalian, 116000, China.
FAU - Qi, Xun
AU  - Qi X
AD  - Department of Radiology, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
AD  - Key Laboratory of Diagnostic Imaging and Interventional Radiology of Liaoning 
      Province, The First Hospital of China Medical University, Shenyang, 110001, 
      China.
FAU - Li, Xue-Yuan
AU  - Li XY
AD  - Department of Cardiology, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
FAU - Wang, Xiao-Bing
AU  - Wang XB
AD  - Department of Echocardiography, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
FAU - Lv, Feng
AU  - Lv F
AD  - Institute of Biomedical Engineering, Peking Union Medical College, Beijing, 
      100010, China.
FAU - Yu, Lei
AU  - Yu L
AD  - Department of Cardiac Surgery, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
FAU - Jiang, Da-Qing
AU  - Jiang DQ
AD  - Department of Cardiac Surgery, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
FAU - Tang, Rui
AU  - Tang R
AD  - Department of Cardiac Surgery, The First Hospital of China Medical University, 
      Shenyang, 110001, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151015
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Bone Marrow Cells/cytology
MH  - Bone Marrow Transplantation
MH  - Cell Differentiation/drug effects
MH  - Cell Hypoxia/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Coronary Vessels/surgery
MH  - Disease Models, Animal
MH  - Drug Therapy, Combination
MH  - Hepatocyte Growth Factor/*therapeutic use
MH  - Insulin-Like Growth Factor I/*therapeutic use
MH  - Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Myocardial Infarction/*drug therapy
MH  - Myocytes, Cardiac/*cytology
MH  - Neovascularization, Physiologic/drug effects
MH  - Rabbits
PMC - PMC6496765
COIS- None declared.
EDAT- 2015/10/16 06:00
MHDA- 2016/04/27 06:00
PMCR- 2015/10/15
CRDT- 2015/10/16 06:00
PHST- 2015/05/27 00:00 [received]
PHST- 2015/07/29 00:00 [accepted]
PHST- 2015/10/16 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/04/27 06:00 [medline]
PHST- 2015/10/15 00:00 [pmc-release]
AID - CPR12219 [pii]
AID - 10.1111/cpr.12219 [doi]
PST - ppublish
SO  - Cell Prolif. 2015 Dec;48(6):661-70. doi: 10.1111/cpr.12219. Epub 2015 Oct 15.