PMID- 26467133
OWN - NLM
STAT- MEDLINE
DCOM- 20151112
LR  - 20221207
IS  - 1899-5276 (Print)
IS  - 1899-5276 (Linking)
VI  - 24
IP  - 3
DP  - 2015 May-Jun
TI  - An Assay of Selected Serum Amino Acids in Patients with Type 2 Diabetes Mellitus.
PG  - 447-51
LID - 10.17219/acem/29223 [doi]
AB  - BACKGROUND: Amino acids are the building blocks of proteins. In case of insulin 
      resistance, which is typical for type 2 diabetes mellitus (T2DM), proteolysis is 
      increased and protein synthesis is decreased; therefore, we can observe changes 
      in the levels of amino acids in diabetics vs. non-diabetics. OBJECTIVES: The aim 
      of this study was to find differences in the levels of selected amino acids 
      between patients with diabetes (type 2) and a control group. MATERIAL AND 
      METHODS: Amino acids were derivatized with naphthalene-2,3-dicarboxaldehyde in 
      the presence of potassium cyanide to form fluorescent 1-cyanobenz(f)isoindole 
      product. Amino acids derivatives were measured using a high-performance liquid 
      chromatography with fluorescence detection. The serum levels of glucose were 
      determined using an automatic biochemistry analyzer, glycated hemoglobin HbA1c 
      was measured by cation exchange chromatography. RESULTS: A total of 19 serum 
      amino acids in T2DM patients and non-diabetics were measured. There were 9 amino 
      acids, which were significantly different in these groups (p<0.05). Significantly 
      decreased levels of arginine, asparagine, glycine, serine, threonine and 
      significantly increased levels of alanine, isoleucine, leucine, valine in 
      diabetics were found. CONCLUSIONS: Significant difference in metabolism of amino 
      acids between diabetics and non-diabetics were observed. The altered levels of 
      amino acids in diabetic patients could be a suitable predictor of diabetes.
FAU - Drabkova, Petra
AU  - Drabkova P
AD  - Department of Biological and Biochemical Sciences, Faculty of Chemical 
      Technology, University of Pardubice, Czech Republic.
FAU - Sanderova, Jana
AU  - Sanderova J
AD  - Department of Biological and Biochemical Sciences, Faculty of Chemical 
      Technology, University of Pardubice, Czech Republic.
FAU - Kovarik, Jakub
AU  - Kovarik J
AD  - Department of Clinical Biochemistry and Diagnostics, Faculty of Chemical 
      Technology, Regional Hospital Pardubice, Czech Republic.
FAU - kandar, Roman
AU  - kandar R
AD  - Department of Biological and Biochemical Sciences, Faculty of Chemical 
      Technology, University of Pardubice, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Adv Clin Exp Med
JT  - Advances in clinical and experimental medicine : official organ Wroclaw Medical 
      University
JID - 101138582
RN  - 0 (Amino Acids)
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (hemoglobin A1c protein, human)
SB  - IM
MH  - Aged
MH  - Amino Acids/*blood
MH  - Automation, Laboratory
MH  - Biomarkers/blood
MH  - Blood Glucose/analysis
MH  - Case-Control Studies
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Ion Exchange
MH  - Diabetes Mellitus, Type 2/*blood/diagnosis
MH  - Female
MH  - Glycated Hemoglobin/analysis
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Spectrometry, Fluorescence
EDAT- 2015/10/16 06:00
MHDA- 2015/11/13 06:00
CRDT- 2015/10/16 06:00
PHST- 2015/10/16 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2015/11/13 06:00 [medline]
AID - 10.17219/acem/29223 [doi]
PST - ppublish
SO  - Adv Clin Exp Med. 2015 May-Jun;24(3):447-51. doi: 10.17219/acem/29223.