PMID- 26467281
OWN - NLM
STAT- MEDLINE
DCOM- 20161021
LR  - 20191113
IS  - 2352-0930 (Electronic)
VI  - 9
IP  - 1
DP  - 2015
TI  - Susceptibility Risk Alleles of -238G/A, -308G/A and -1031T/C Promoter 
      Polymorphisms of TNF-alpha Gene to Uterine Leiomyomas.
PG  - 65-71
AB  - BACKGROUND: Uterine Leiomyomas (UL) are non-cancerous single celled mass of 
      uterine smooth muscles distinguished by presence of large amounts of collagen, 
      fibronectin and proteoglycans. Tumor necrosis factor-alpha (TNF-alpha), an inflammation 
      inducing cytokine, plays a major role in various disorders of the immune system; 
      is involved in tumor development and progression. It is proposed to study the 
      influence of three functional promoter polymorphisms of TNF-alpha viz -238G/A, 
      -308G/A and -1031T/C in the development and progression of UL. METHODOLOGY: Study 
      included 146 individuals positive for uterine fibroids and 150 healthy 
      individuals. Genomic DNA was isolated from white blood corpuscles and subjected 
      to PCR-RFLP analysis and Allele Specific PCR (ARMS). The significance of the 
      obtained data in controls and patients was estimated and computed by adopting 
      appropriate statistical tools. RESULTS: In this study an association between 
      TNF-alpha -1031T/C polymorphism and UL was reported. A significant association of the 
      TC genotype (chi(2) - 14.34; p=0.0008) and the C allele (chi(2) - 5.898 p=0.015) with 
      uterine leiomyomas was observed. Likewise odds risk estimates of 2.56 (95% CI 
      1.56-4.20, p=0.0007) revealed a significant association of TC genotype and C 
      allele with uterine leiomyomas. CONCLUSIONS: "TC" genotype and "C" allele of 
      rs1799964 (-1031T/C) is associated with higher risks to leiomyomas. The "C" 
      allele of -1031T/C results in an increased expression TNF-alpha leading to smooth 
      cell proliferation and tumor progression, hence, may be a relevant molecular 
      marker in the identification and establishment of UL.
FAU - Medikare, Veronica
AU  - Medikare V
FAU - Ali, Altaf
AU  - Ali A
FAU - Ananthapur, Venkateshwari
AU  - Ananthapur V
FAU - Deendayal, Mamata
AU  - Deendayal M
FAU - Nallari, Pratibha
AU  - Nallari P
AD  - Department of Genetics, University College of Science, Osmania University, 
      Hyderabad-500007, Telangana, India. prathinallari@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United Arab Emirates
TA  - Recent Adv DNA Gene Seq
JT  - Recent advances in DNA & gene sequences
JID - 101648465
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Alleles
MH  - Female
MH  - *Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Genotype
MH  - Humans
MH  - Leiomyoma/*genetics/pathology
MH  - Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - Risk Factors
MH  - Tumor Necrosis Factor-alpha/*genetics
MH  - Uterine Neoplasms/*genetics/pathology
EDAT- 2015/10/16 06:00
MHDA- 2016/10/22 06:00
CRDT- 2015/10/16 06:00
PHST- 2015/10/16 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/10/22 06:00 [medline]
AID - CMC-EPUB-71110 [pii]
AID - 10.2174/2352092210999151214155858 [doi]
PST - ppublish
SO  - Recent Adv DNA Gene Seq. 2015;9(1):65-71. doi: 10.2174/2352092210999151214155858.