PMID- 26468789
OWN - NLM
STAT- MEDLINE
DCOM- 20160620
LR  - 20181202
IS  - 1536-3732 (Electronic)
IS  - 1049-2275 (Linking)
VI  - 26
IP  - 7
DP  - 2015 Oct
TI  - The Effect of Tumor Necrosis Factor-alpha at Different Concentrations on Osteogenetic 
      Differentiation of Bone Marrow Mesenchymal Stem Cells.
PG  - 2081-5
LID - 10.1097/SCS.0000000000001971 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) has been demonstrated to have a close 
      relationship with inflammation in the body. Although most researchers confirmed 
      that TNF-alpha can have an effect on the expression of osteoblast specific genes, 
      they had not elucidated the regulation of inflammatory factors in osteogenic gene 
      expression during the process of bone marrow mesenchymal stem cells (BMMSCs) 
      differentiating to osteoblast. The aim of this study was to investigate the 
      effect of TNF-alpha at different concentrations on osteogenetic differentiation of 
      BMMSCs. In this study, BMMSCs proliferation was analyzed by using cell counting 
      kit-8 assay, cell osteogenic differentiation was evaluated by means of alkaline 
      phosphatase activity assay and Von Koaas staining and the messenger RNA (mRNA) 
      expression of bone morphogenetic proteins-2 (BMP-2) and drosophila mothers 
      against decapentaplegic protein 1 (Smad1) was measured through real-time 
      polymerase chain reaction. The results indicated that a low concentration of 
      TNF-alpha at short-term promotes the osteogenetic differentiation of BMMSCs and 
      increases the mRNA expression of BMP-2 and Smad1, but inhibits the osteogenetic 
      differentiation of BMMSCs and the expression of BMP-2 and Smad1 at long term. In 
      addition, regardless of a short or long time, a high concentration of TNF-alpha 
      inhibits the osteogenetic differentiation of BMMSCs and the expression of Smad1, 
      but results in a high expression of BMP-2.
FAU - Wang, You-wei
AU  - Wang YW
AD  - Department of Oral and Maxillofacial Surgery, School of Stomatology, China 
      Medical University, Shenyang, Liaoning province, PR China.
FAU - Xu, Da-peng
AU  - Xu DP
FAU - Liu, Yang
AU  - Liu Y
FAU - Zhang, Ran
AU  - Zhang R
FAU - Lu, Li
AU  - Lu L
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Craniofac Surg
JT  - The Journal of craniofacial surgery
JID - 9010410
RN  - 0 (Anthraquinones)
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Coloring Agents)
RN  - 0 (Smad1 Protein)
RN  - 0 (Smad1 protein, rat)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 60MEW57T9G (alizarin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
MH  - Alkaline Phosphatase/analysis
MH  - Animals
MH  - Anthraquinones
MH  - Bone Morphogenetic Protein 2/analysis/drug effects
MH  - Calcification, Physiologic/drug effects
MH  - Cell Count
MH  - Cell Culture Techniques
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Shape/drug effects
MH  - Coloring Agents
MH  - Female
MH  - Male
MH  - Mesenchymal Stem Cells/*drug effects
MH  - Osteoblasts/drug effects/physiology
MH  - Osteogenesis/*drug effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/drug effects
MH  - Smad1 Protein/analysis/drug effects
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*administration & dosage
EDAT- 2015/10/16 06:00
MHDA- 2016/06/21 06:00
CRDT- 2015/10/16 06:00
PHST- 2015/10/16 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/06/21 06:00 [medline]
AID - 00001665-201510000-00011 [pii]
AID - 10.1097/SCS.0000000000001971 [doi]
PST - ppublish
SO  - J Craniofac Surg. 2015 Oct;26(7):2081-5. doi: 10.1097/SCS.0000000000001971.