PMID- 26468840
OWN - NLM
STAT- MEDLINE
DCOM- 20160630
LR  - 20181202
IS  - 1536-3732 (Electronic)
IS  - 1049-2275 (Linking)
VI  - 26
IP  - 7
DP  - 2015 Oct
TI  - Comparing Viability of Periodontal Ligament Stem Cells Isolated From Erupted and 
      Impacted Tooth Root.
PG  - e608-12
LID - 10.1097/SCS.0000000000002112 [doi]
AB  - PURPOSE: The aim of the study was to compare the viability of periodontal 
      ligament-derived stem/progenitor cells (PDLSCs) from 2 different sources. 
      MATERIALS AND METHODS: Periodontal ligament (PDL) tissue was obtained from 20 
      surgically extracted human third molars and 20 healthy premolars extracted for 
      orthodontic reasons. Periodontal ligament-derived stem/progenitor cells were 
      isolated from 2 different PDL tissue sources and characterized by colony forming 
      unit assay, cell surface marker characterizations, and their osteogenic 
      differentiation potential. To determine cell viability within 2 groups, the 
      colorimetric 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide 
      (MTT) metabolic activity assay was used. Data were statistically analyzed using 
      independent t-test by SPSS 16 software (SPSS Inc, Chicago, IL). RESULTS: 
      According to the MTT assay, the mean viability rate +/- standard deviation of 
      PDLSCs in the impacted third molar sample cells was 0.355 +/- 0.411 and for erupted 
      premolar sample cells was 0.331 +/- 0.556. Based on One-Sample Kolmogorov-Smirnov 
      test, P value for impacted and erupted teeth was 0.954 and 0.863, respectively. 
      No statistical difference was seen between 2 groups. (P value > 0.05) 
      CONCLUSIONS: Our results demonstrated that if surgical aseptic technique is a 
      method employed to maintain asepsis, PDLSCs obtained from impacted and erupted 
      tooth root would have the same viability rate.
FAU - Dalband, Mohsen
AU  - Dalband M
AD  - *Department of Oral and Maxillofacial Surgery, Dental Faculty, Shahid Beheshti 
      University of Medical Science, Tehran daggerDepartment of Anatomy and Embryology, 
      Medical Faculty, Hamadan University of Medical Sciences double daggerDepartment of 
      Biostatistics and Epidemiology, Research Center for Modeling of Noncommunicable 
      Diseases, Public Health Faculty, Hamadan University of Medical Sciences, Hamadan 
       section signDepartment of Oral and Maxillofacial Surgery, Maxillofacial Diseases Research 
      Center, Dental Faculty, Mashhad University of Medical Science, Mashhad 
      ||Department of Oral and Maxillofacial Surgery, Dental Faculty, Kurdestan 
      University of Medical Science, Sanandaj  paragraph signPrivate Practice, Hamadan, Iran.
FAU - Amiri, Iraj
AU  - Amiri I
FAU - Soltanian, Ali Reza
AU  - Soltanian AR
FAU - Gholami, Mehdi
AU  - Gholami M
FAU - Khayati, Adell
AU  - Khayati A
FAU - Keshmirypor, Vahid
AU  - Keshmirypor V
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Craniofac Surg
JT  - The Journal of craniofacial surgery
JID - 9010410
RN  - 0 (Coloring Agents)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - EUY85H477I (thiazolyl blue)
MH  - Adolescent
MH  - Adult
MH  - Bicuspid/cytology
MH  - Cell Culture Techniques
MH  - Cell Differentiation/physiology
MH  - Cell Shape
MH  - Cell Survival/physiology
MH  - Colorimetry/methods
MH  - Coloring Agents
MH  - Fibroblasts/physiology
MH  - Humans
MH  - Mesenchymal Stem Cells/physiology
MH  - Molar, Third/pathology
MH  - Osteogenesis/physiology
MH  - Periodontal Ligament/*cytology
MH  - Stem Cells/*physiology
MH  - Tetrazolium Salts
MH  - Thiazoles
MH  - Tooth Eruption/physiology
MH  - Tooth Root/*cytology
MH  - Tooth, Impacted/*pathology
MH  - Young Adult
EDAT- 2015/10/16 06:00
MHDA- 2016/07/01 06:00
CRDT- 2015/10/16 06:00
PHST- 2015/10/16 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2016/07/01 06:00 [medline]
AID - 00001665-201510000-00085 [pii]
AID - 10.1097/SCS.0000000000002112 [doi]
PST - ppublish
SO  - J Craniofac Surg. 2015 Oct;26(7):e608-12. doi: 10.1097/SCS.0000000000002112.