PMID- 26469118
OWN - NLM
STAT- MEDLINE
DCOM- 20151123
LR  - 20181023
IS  - 1899-5276 (Print)
IS  - 1899-5276 (Linking)
VI  - 24
IP  - 4
DP  - 2015 Jul-Aug
TI  - Detection of Chromosomal Abnormalities with Different In Situ Hybridisation 
      Techniques--the Usefulness in the Qualification of Cancer Patients for 
      Molecularly-Targeted Therapies.
PG  - 715-23
LID - 10.17219/acem/28825 [doi]
AB  - Proper qualification of patients with cancer for an effective treatment regiment 
      is essential to rationalize therapy benefit and costs. The early detection of 
      genetic disorders that are responsible for the stimulation of uncontrolled cancer 
      cells proliferation makes it possible to select a group of patients with a high 
      probability of response to molecularly-targeted therapy. Data has shown that 
      careful analysis of genes mutation using different PCR and sequencing techniques 
      or chromosomal aberrations using in situ hybridization (ISH) techniques have 
      a predictive value for drug targeted therapy. Overexpression of receptors and 
      gene amplification has been reported in various cancers. Their detection is still 
      a considerable challenge, which is connected with the unsatisfactory quality of 
      DNA and low mutated cells percentage compared to cells with no genetic 
      abnormalities in tested material. Different techniques of standardization were 
      performed to prevent false negative results and to increase the sensitivity of 
      qualitative and quantitative evaluation of chromosomal abnormalities. 
      Immunohistochemistry (IHC) technique is useful in the screening of receptor 
      expression in paraffin-embedded tissue samples in different malignant diseases. 
      Whereas ISH techniques, especially fluorescence in situ hybridization (FISH), are 
      now considered the diagnostic gold standard method in detection chromosomal 
      aberrations. Moreover, molecular biology techniques, which are using molecular 
      probes and real-time PCR and quantitative PCR techniques, were also applied for 
      the detection of chromosomal changes. In order to identify the best genetic 
      marker for treatment regiment, it is important to compare results of different 
      studies, which are evaluating the sensitivity of diagnostic techniques and 
      treatment response after a suitable selection factors based on genetic 
      aberrations profile.
FAU - Nicos, Marcin
AU  - Nicos M
AD  - Department of Pneumonology, Oncology and Allergology, Medical University of 
      Lublin Postgraduate School of Molecular Medicine, Medical University of Warsaw, 
      Poland.
FAU - Wojas-Krawczyk, Kamila
AU  - Wojas-Krawczyk K
AD  - Department of Pneumonology, Oncology and Allergology, Medical University of 
      Lublin, Poland.
FAU - Krawczyk, Pawel
AU  - Krawczyk P
AD  - Department of Pneumonology, Oncology and Allergology, Medical University of 
      Lublin, Poland.
FAU - Milanowski, Janusz
AU  - Milanowski J
AD  - Department of Pneumonology, Oncology and Allergology, Medical University of 
      Lublin, Poland.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Adv Clin Exp Med
JT  - Advances in clinical and experimental medicine : official organ Wroclaw Medical 
      University
JID - 101138582
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Antineoplastic Agents/*therapeutic use
MH  - Biomarkers, Tumor/*genetics
MH  - *Chromosome Aberrations
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence
MH  - *Molecular Targeted Therapy
MH  - Neoplasms/*drug therapy/*genetics/pathology
MH  - Patient Selection
MH  - Phenotype
MH  - *Precision Medicine
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
EDAT- 2015/10/16 06:00
MHDA- 2015/12/15 06:00
CRDT- 2015/10/16 06:00
PHST- 2015/10/16 06:00 [entrez]
PHST- 2015/10/16 06:00 [pubmed]
PHST- 2015/12/15 06:00 [medline]
AID - 10.17219/acem/28825 [doi]
PST - ppublish
SO  - Adv Clin Exp Med. 2015 Jul-Aug;24(4):715-23. doi: 10.17219/acem/28825.