PMID- 26472277
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR  - 20161230
IS  - 1879-1336 (Electronic)
IS  - 1054-8807 (Linking)
VI  - 25
IP  - 1
DP  - 2016 Jan-Feb
TI  - Ultrastructural morphometric findings of cardiomyocytes in patients with impaired 
      ventricular function--a comparative clinicopathological study.
PG  - 25-32
LID - S1054-8807(15)00116-7 [pii]
LID - 10.1016/j.carpath.2015.09.005 [doi]
AB  - AIM: The present study aims to analyze the differences in ultrastructural changes 
      between right ventricular myocardium in clinically determined grades of heart 
      failure (HF) [New York Heart Association (NYHA) classes I-IV] and their value in 
      the routine diagnostic setting. METHODS: We investigated consecutive right 
      ventricular endomyocardial biopsies of 12 patients presenting with HF 
      (49+/-11.2years; male=10) by light microscopy and ultrastructural morphometric 
      analysis. The patients were divided into four groups according to their NYHA 
      classes (NYHA I: n=1, II: n=2, III: n=8, IV: n=1). We used a stereological point 
      counting method on electron micrographs to determine the volume, surface, and 
      numerical density of cardiomyocyte myofibrils; z-lines; mitochondria; and cristae 
      as required. Further, secondary parameters were calculated. RESULTS: Myofibrillar 
      parameters increased between NYHA class I and II (P<.01), which matched with more 
      pronounced cardiomyocyte hypertrophy on the light microscopic level. In NYHA 
      classes III and IV, the myofibrillar parameters dropped, while parameters 
      concerning the mitochondria and their cristae rose (P<.01). This resulted in an 
      elevated mitochondria to myofibril ratio (P<.05) and correlated with 
      histologically evident atrophic cardiomyocytes, perinuclear loss of myofibrils 
      and dot-like perinuclear staining positive on peroxide acid shift. CONCLUSION: In 
      this present study, right ventricular myocardial ultrastructure differed between 
      patients diagnosed with HF of different degrees in distinct subcellular changes. 
      These findings suggest that ultrastructural analysis, while correlated with 
      histopathological features, adds to the diagnosis in the routine diagnostic 
      setting, specifically in lower NYHA grades, in which only minor changes are 
      observed histologically.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Wassilew, Katharina
AU  - Wassilew K
AD  - Cardiac Pathology Unit, Department of Cardiothoracic and Vascular Surgery, 
      Deutsches Herzzentrum Berlin, Berlin, Germany. Electronic address: 
      wassilew@dhzb.de.
FAU - Terziev, Denis
AU  - Terziev D
AD  - Institute of Anatomy, Medical University Leipzig, Leipzig, Germany.
FAU - Wassilew, Georgi
AU  - Wassilew G
AD  - Private Histopathology Laboratory, Berlin, Germany.
FAU - Fitzl, Gunther
AU  - Fitzl G
AD  - Institute of Anatomy, Medical University Leipzig, Leipzig, Germany.
FAU - Frolich, Karsten
AU  - Frolich K
AD  - FNS Personal und Informationssysteme GmbH, Berlin, Germany.
FAU - Kandolf, Reinhard
AU  - Kandolf R
AD  - Department of Molecular Pathology, Institute of Pathology and Neuropathology, 
      Tubingen, Germany.
FAU - Fried, Andreas
AU  - Fried A
AD  - Department of Cardiology, Hospital Havelhoehe, Berlin, Germany.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20150926
PL  - United States
TA  - Cardiovasc Pathol
JT  - Cardiovascular pathology : the official journal of the Society for Cardiovascular 
      Pathology
JID - 9212060
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biopsy
MH  - DNA, Viral/genetics
MH  - Female
MH  - Heart Failure/*pathology/physiopathology/virology
MH  - Humans
MH  - Male
MH  - Microscopy, Electron
MH  - Middle Aged
MH  - Mitochondria, Heart/ultrastructure
MH  - Myocytes, Cardiac/*ultrastructure/virology
MH  - Myofibrils/ultrastructure
MH  - Ventricular Dysfunction, Right/*pathology/physiopathology/virology
MH  - Ventricular Function, Right
OTO - NOTNLM
OT  - Electron microscopy
OT  - Heart failure
OT  - Stereology
EDAT- 2015/10/17 06:00
MHDA- 2016/10/07 06:00
CRDT- 2015/10/17 06:00
PHST- 2015/07/13 00:00 [received]
PHST- 2015/09/01 00:00 [revised]
PHST- 2015/09/20 00:00 [accepted]
PHST- 2015/10/17 06:00 [entrez]
PHST- 2015/10/17 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
AID - S1054-8807(15)00116-7 [pii]
AID - 10.1016/j.carpath.2015.09.005 [doi]
PST - ppublish
SO  - Cardiovasc Pathol. 2016 Jan-Feb;25(1):25-32. doi: 10.1016/j.carpath.2015.09.005. 
      Epub 2015 Sep 26.