PMID- 26472457
OWN - NLM
STAT- MEDLINE
DCOM- 20160419
LR  - 20181113
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 1364
DP  - 2016
TI  - Intravascular AAV9 Administration for Delivering RNA Silencing Constructs to the 
      CNS and Periphery.
PG  - 261-75
LID - 10.1007/978-1-4939-3112-5_21 [doi]
AB  - Viral vector delivery of RNA silencing constructs, when administered into 
      vasculature, typically results in poor central nervous system (CNS) transduction 
      due to the inability of the vector to cross the blood-brain barrier (BBB). 
      However, adeno-associated virus serotype 9 (AAV9) has the ability to cross the 
      BBB and robustly transduce brain parenchyma and peripheral tissues at 
      biologically meaningful levels when injected intravenously. Recent work by our 
      lab has shown that this method can be used to deliver RNA silencing constructs, 
      resulting in significant reductions in gene expression in multiple brain regions 
      and in peripheral tissues. Here, we outline a method for delivery of AAV9 vectors 
      expressing RNA interference (RNAi) constructs that lead to robust simultaneous 
      transduction of mouse peripheral tissues and the CNS following a single injection 
      into the jugular vein. Additionally, we outline methods for necropsy and 
      immunofluorescence to detect AAV9 transduction patterns in the rodent CNS 
      following a vascular delivery.
FAU - Dufour, Brett D
AU  - Dufour BD
AD  - Division of Neuroscience, Oregon National Primate Research Center, Beaverton, OR, 
      USA.
AD  - Department of Behavioral Neuroscience, Oregon Health and Science University, 
      Portland, OR, USA.
FAU - McBride, Jodi L
AU  - McBride JL
AD  - Division of Neuroscience, Oregon National Primate Research Center, Beaverton, OR, 
      USA. mcbridej@ohsu.edu.
AD  - Department of Behavioral Neuroscience, Oregon Health and Science University, 
      Portland, OR, USA. mcbridej@ohsu.edu.
AD  - Department of Neurology, Oregon Health and Science University, Portland, OR, USA. 
      mcbridej@ohsu.edu.
LA  - eng
GR  - P51 OD011092/OD/NIH HHS/United States
GR  - P51 RR000163/RR/NCRR NIH HHS/United States
GR  - NS7466-14/NS/NINDS NIH HHS/United States
GR  - S10RR024585/RR/NCRR NIH HHS/United States
GR  - K01 RR000163/RR/NCRR NIH HHS/United States
GR  - RR000163/RR/NCRR NIH HHS/United States
GR  - T32 NS007466/NS/NINDS NIH HHS/United States
GR  - S10 RR024585/RR/NCRR NIH HHS/United States
GR  - P30 NS061800/NS/NINDS NIH HHS/United States
GR  - P51OD011092/OD/NIH HHS/United States
GR  - P30-NS061800/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Brain/cytology/*metabolism/virology
MH  - Dependovirus/*genetics
MH  - Fluorescent Antibody Technique
MH  - Genetic Vectors/*genetics
MH  - Injections
MH  - *Jugular Veins
MH  - Mice
MH  - *RNA Interference
MH  - Transduction, Genetic
PMC - PMC5519340
MID - NIHMS873105
OTO - NOTNLM
OT  - AAV9, systemic
OT  - Gene therapy
OT  - Jugular vein
OT  - RNAi
OT  - Vascular
EDAT- 2015/10/17 06:00
MHDA- 2016/04/20 06:00
PMCR- 2017/07/20
CRDT- 2015/10/17 06:00
PHST- 2015/10/17 06:00 [entrez]
PHST- 2015/10/17 06:00 [pubmed]
PHST- 2016/04/20 06:00 [medline]
PHST- 2017/07/20 00:00 [pmc-release]
AID - 10.1007/978-1-4939-3112-5_21 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1364:261-75. doi: 10.1007/978-1-4939-3112-5_21.