PMID- 26472578
OWN - NLM
STAT- MEDLINE
DCOM- 20160502
LR  - 20221207
IS  - 1574-6941 (Electronic)
IS  - 0168-6496 (Linking)
VI  - 91
IP  - 11
DP  - 2015 Nov
TI  - Predation of nitritation-anammox biofilms used for nitrogen removal from 
      wastewater.
LID - fiv124 [pii]
LID - 10.1093/femsec/fiv124 [doi]
AB  - Predation is assumed to be a major cause of bacterial mortality in wastewater 
      treatment plants (WWTP). Grazing on the slowly growing autotrophic ammonia 
      oxidizing bacteria (AOB) and anaerobic ammonium oxidizing bacteria (AMX) may 
      result in loss of biomass, which could compromise nitrogen removal by the 
      nitritation-anammox process. However, predation, particularly of anaerobic AMX, 
      is unknown. We investigated the presence of protozoa, AOB and AMX and the 
      possible predation in nitritation-anammox biofilms from several WWTPs. By 
      fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy 
      (CLSM), predator and prey were localized in intact biofilm cryosections. 
      Different broad morphological types of protozoa were found at different biofilm 
      depths. Large variations in abundance of protozoa were seen. One reactor showed a 
      predation event of amoeba-like protozoa, forming grazing fronts reaching deep 
      biofilm regions that were dominated by the anaerobic AMX. Both AOB and AMX were 
      grazed by the amoeba, as revealed by FISH-CLSM. Hence, even AMX, living in the 
      deeper layers of stratified biofilms, are subjected to predation. Interestingly, 
      different colocalization was observed between the amoeba-like protozoa and two 
      different Ca. Brocadia AMX sublineages, indicating different grazing patterns. 
      The findings indicate that predation pressure can be an important factor 
      regulating the abundance of AOB and AMX, with implications for nitrogen removal 
      from wastewater.
CI  - (c) FEMS 2015. All rights reserved. For permissions, please e-mail: 
      journals.permissions@oup.com.
FAU - Suarez, Carolina
AU  - Suarez C
AD  - Department of Chemistry and Molecular Biology/Microbiology, University of 
      Gothenburg, SE-405 30, Sweden.
FAU - Persson, Frank
AU  - Persson F
AD  - Water Environment Technology, Department of Civil and Environmental Engineering, 
      Chalmers University of Technology, SE-412 96, Sweden.
FAU - Hermansson, Malte
AU  - Hermansson M
AD  - Department of Chemistry and Molecular Biology/Microbiology, University of 
      Gothenburg, SE-405 30, Sweden malte.hermansson@cmb.gu.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151014
PL  - England
TA  - FEMS Microbiol Ecol
JT  - FEMS microbiology ecology
JID - 8901229
RN  - 0 (Waste Water)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Bacteria/classification/*metabolism
MH  - Bacteria, Anaerobic/genetics
MH  - *Biofilms
MH  - Denitrification
MH  - Eukaryota/*classification/*physiology
MH  - *Food Chain
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Confocal
MH  - Nitrogen/analysis
MH  - Oxidation-Reduction
MH  - Wastewater/*microbiology
MH  - *Water Purification
OTO - NOTNLM
OT  - FISH-CLSM
OT  - MBBR
OT  - biofilm
OT  - nitritation-anammox
OT  - predation
EDAT- 2015/10/17 06:00
MHDA- 2016/05/03 06:00
CRDT- 2015/10/17 06:00
PHST- 2015/10/08 00:00 [accepted]
PHST- 2015/10/17 06:00 [entrez]
PHST- 2015/10/17 06:00 [pubmed]
PHST- 2016/05/03 06:00 [medline]
AID - fiv124 [pii]
AID - 10.1093/femsec/fiv124 [doi]
PST - ppublish
SO  - FEMS Microbiol Ecol. 2015 Nov;91(11):fiv124. doi: 10.1093/femsec/fiv124. Epub 
      2015 Oct 14.