PMID- 26472618
OWN - NLM
STAT- MEDLINE
DCOM- 20160926
LR  - 20151219
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 55
DP  - 2016 Feb
TI  - Inflammatory immune response by lipopolysaccharide-responsive nucleotide binding 
      oligomerization domain (NOD)-like receptors in the Japanese pufferfish (Takifugu 
      rubripes).
PG  - 21-31
LID - S0145-305X(15)30052-5 [pii]
LID - 10.1016/j.dci.2015.10.008 [doi]
AB  - Some of NOD-like receptors (NLRs), the cytosolic pattern recognition receptors 
      form a multi-protein complex, inflammasome consisting of one or more NLRs, the 
      adaptor protein ASC and inflammatory caspase to generate mature inflammatory 
      cytokines, interleukin (IL)-1beta and IL-18. However, inflammasome-mediated 
      inflammatory cascade involving any NLR member is unknown in a lower vertebrate 
      like fish. Also, inflammatory cytokine induction pathway in response to a 
      specific ligand, namely bacterial lipopolysaccharide (LPS) has not yet been 
      clarified. Therefore, 13 predicted NLR sequences of the Japanese pufferfish, Fugu 
      (Takifugu rubripes) were retrieved in silico and categorized as NLR-C1 approximately 13. 
      Expression analysis of these genes in Fugu head kidney (HK) cells stimulated with 
      a heat-killed Lactobacillus paracasei spp. paracasei (Lpp), LPS, nigericin and a 
      combination of nigericin + LPS showed consistent up-regulations of NLR-C1, 5, 7, 
      10 and 12 genes in both Lpp and LPS stimulations and NLR-C9 gene in LPS 
      stimulation only. However, nigericin and nigericin + LPS caused an increased 
      expression of NLR-C10 and 12 in HK cells and leukocytes. Fugu treated with Lpp 
      and LPS (in vivo), and infected with Vibrio harveyi had an elevated expression of 
      NLR-C10 and 12. Increased transcription of caspase-1, ASC, IL-1beta and IL-18 was 
      recorded in nigericin-stimulated HK cells and leukocytes. Results suggested 
      activation of probable inflammasome-mediated inflammatory cytokine response in 
      Fugu. Moreover, LPS may be a key ligand that induces some of the Fugu NLR-Cs 
      (NLR-C9, 10 and 12). Further characterization and functional analysis of Fugu 
      NLR-C10 and 12 for ligand sensing, and processing of pro-inflammatory cytokine, 
      IL-1beta would elucidate the inflammasome evolution in fish.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Biswas, Gouranga
AU  - Biswas G
AD  - Interdisciplinary Graduate School of Agriculture and Engineering, University of 
      Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Bilen, Soner
AU  - Bilen S
AD  - Department of Basic Sciences, Faculty of Fisheries, Kastamonu University, 
      Kastamonu 37200, Turkey.
FAU - Kono, Tomoya
AU  - Kono T
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Sakai, Masahiro
AU  - Sakai M
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan.
FAU - Hikima, Jun-ichi
AU  - Hikima J
AD  - Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, 
      University of Miyazaki, 1-1 Gakuenkibanadai-nishi, Miyazaki 889-2192, Japan. 
      Electronic address: jhikima@cc.miyazaki-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151022
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Fish Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Nod Signaling Adaptor Proteins)
RN  - RRU6GY95IS (Nigericin)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Bacterial/immunology
MH  - Cells, Cultured
MH  - Computational Biology
MH  - Fish Proteins/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Head Kidney/pathology
MH  - Humans
MH  - Inflammation/*immunology
MH  - Lactobacillus/*immunology
MH  - Leukocytes/*immunology
MH  - Lipopolysaccharides/immunology
MH  - Molecular Sequence Data
MH  - Nigericin/immunology
MH  - Nod Signaling Adaptor Proteins/genetics/*metabolism
MH  - Phylogeny
MH  - Tetraodontiformes/*immunology
MH  - Vibrio/*immunology
MH  - Vibrio Infections/*immunology
OTO - NOTNLM
OT  - Inflammasome
OT  - Inflammatory cytokines
OT  - Intracellular PRRs
OT  - Japanese pufferfish
OT  - LPS recognition
OT  - NLR
EDAT- 2015/10/17 06:00
MHDA- 2016/09/27 06:00
CRDT- 2015/10/17 06:00
PHST- 2015/07/05 00:00 [received]
PHST- 2015/10/07 00:00 [revised]
PHST- 2015/10/07 00:00 [accepted]
PHST- 2015/10/17 06:00 [entrez]
PHST- 2015/10/17 06:00 [pubmed]
PHST- 2016/09/27 06:00 [medline]
AID - S0145-305X(15)30052-5 [pii]
AID - 10.1016/j.dci.2015.10.008 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2016 Feb;55:21-31. doi: 10.1016/j.dci.2015.10.008. Epub 2015 
      Oct 22.