PMID- 26473079
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151016
LR  - 20201001
IS  - 2074-1804 (Print)
IS  - 2074-1812 (Electronic)
IS  - 2074-1804 (Linking)
VI  - 17
IP  - 9
DP  - 2015 Sep
TI  - The Association between Human Leukocyte Antigen Class II DR3-DQ2 Haplotype and 
      Type 1 Diabetes in Children of the East Azerbaijan State of Iran.
PG  - e28380
LID - 10.5812/ircmj.28380 [doi]
LID - e28380
AB  - BACKGROUND: Type 1 diabetes mellitus (T1D) is an autoimmune disease. Several 
      associations between human leukocyte antigen (HLA) complex and T1D were found in 
      various populations. Associations with various HLA types depend on the 
      investigated populations. However, such associations have not yet been 
      investigated in the East Azerbaijan state of Iran with Turkish ethnicity. 
      OBJECTIVES: The aims of the current study was to describe T1D genetic 
      susceptibility conferred by HLA class II alleles (DRB1*0301, DQA1*0501 and 
      DQB1*0201) and to determine haplotype frequencies among T1D patients. PATIENTS 
      AND METHODS: This study was a case-control study. The number of samples was 
      determined using the Cochran formula. Eighty unrelated T1D patients, including 42 
      (52.5%) females and 38 (47.5%) males, were randomly recruited from the East 
      Azerbaijan state of Iran. Typing of HLA was performed by polymerase chain 
      reaction-sequence-specific priming (PCR-SSP) on DNA extracted from peripheral 
      blood mononuclear cells of 80 unrelated patients and 80 unrelated healthy control 
      donors, who were selected randomly. For haplotype analysis, the logistic 
      regression model was performed that allows joint estimation of Single-nucleotide 
      polymorphisms (SNPs) via haplotypes. RESULTS: The frequency of drb1*0301 (82.5% 
      vs. 11.3%), dqa1*0501 (82.5% vs. 36.3%) and dqb1*0201 (81.3% vs. 35%) were 
      significantly higher among patients compared with that of healthy subjects. 
      CONCLUSIONS: Our investigation demonstrated that there is a highly significant 
      association between the studied alleles and T1D. It can be construed that 
      haplotype HLA-DR3-DQ2 has a very modest effect with respect to the risk of T1D.
FAU - Mansoori Derakhshan, Sima
AU  - Mansoori Derakhshan S
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR 
      Iran ; Department of Medical Genetic, Faculty of Medicine, Tabriz University of 
      Medical Sciences, Tabriz, IR Iran.
FAU - Zeinali Sehrig, Fatemeh
AU  - Zeinali Sehrig F
AD  - Department of Biological Science, Ahar Branch, Islamic Azad University, Ahar, IR 
      Iran.
FAU - Sohrabi, Nasrin
AU  - Sohrabi N
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR 
      Iran ; Department of Medical Genetic, Faculty of Medicine, Tabriz University of 
      Medical Sciences, Tabriz, IR Iran.
FAU - Shiva, Siamak
AU  - Shiva S
AD  - Department of Pediatrics, Faculty of Medicine, Tabriz University of Medical 
      Sciences, Tabriz, IR Iran.
FAU - Baradaran, Behzad
AU  - Baradaran B
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR 
      Iran.
FAU - Shekari Khaniani, Mahmoud
AU  - Shekari Khaniani M
AD  - Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, IR 
      Iran ; Department of Medical Genetic, Faculty of Medicine, Tabriz University of 
      Medical Sciences, Tabriz, IR Iran.
LA  - eng
PT  - Journal Article
DEP - 20150928
PL  - Estonia
TA  - Iran Red Crescent Med J
JT  - Iranian Red Crescent medical journal
JID - 101319850
PMC - PMC4601240
OTO - NOTNLM
OT  - Genotype
OT  - HLA-DQB1
OT  - HLA-DRB1
OT  - Haplotype
EDAT- 2015/10/17 06:00
MHDA- 2015/10/17 06:01
PMCR- 2015/09/01
CRDT- 2015/10/17 06:00
PHST- 2015/03/11 00:00 [received]
PHST- 2015/06/12 00:00 [revised]
PHST- 2015/07/13 00:00 [accepted]
PHST- 2015/10/17 06:00 [entrez]
PHST- 2015/10/17 06:00 [pubmed]
PHST- 2015/10/17 06:01 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
AID - 10.5812/ircmj.28380 [doi]
PST - epublish
SO  - Iran Red Crescent Med J. 2015 Sep 28;17(9):e28380. doi: 10.5812/ircmj.28380. 
      eCollection 2015 Sep.