PMID- 26473344
OWN - NLM
STAT- MEDLINE
DCOM- 20160803
LR  - 20191210
IS  - 2769-819X (Electronic)
IS  - 1532-0820 (Print)
IS  - 1532-0820 (Linking)
VI  - 65
IP  - 5
DP  - 2015 Oct
TI  - Evaluation of Nonalcoholic Fatty Liver Disease in C57BL/6J Mice by Using MRI and 
      Histopathologic Analyses.
PG  - 409-15
AB  - Nonalcoholic fatty liver disease (NAFLD) can lead to cirrhosis, hepatocellular 
      carcinoma, and ultimately death. Magnetic resonance techniques are accurate, 
      noninvasive methods for evaluating hepatic steatosis but, in animals, have not 
      been fully validated against histologic findings. We sought to validate the MRI 
      fat-signal fraction (MRI-FSF) used for diagnosing NAFLD in human nonclinical 
      trials by comparing MRI data with histopathologic findings in C57BL/6J mice (n = 
      24) fed normal chow (controls) or a methionine- and choline-deficient (MCD) diet 
      to induce NAFLD. Axial T2-weighted fast spin-echo images were used to examine the 
      entire liver. For histopathologic analyses, liver slides were evaluated for 
      hepatic steatosis according to the NAFLD activity score. Pearson correlation 
      coefficient and receiver operating characteristics analyses were performed. 
      According to the fat-fraction signal, the mean percentage of liver fat in mice 
      with induced NAFLD was 57%, which correlated with the histologically determined 
      steatosis grade. The proton-density fat fraction effectively distinguished severe 
      from mild hepatic steatosis, with an AUC of 0.92. Evaluation accuracy decreased 
      when lobular inflammation and hepatocellular ballooning were considered. This 
      study showed strong concurrence between MRI-FSF and histopathologic steatosis in 
      a murine model of NAFLD. MRI-FSF had moderate sensitivity and specificity in this 
      context. These results confirm that the MRI is a useful biomarker of hepatic 
      steatosis in NAFLD in murine model.
FAU - Ryu, Jae-Eun
AU  - Ryu JE
AD  - Asan Institute for Life Sciences, Asan Medical Center, Seoul 138-736, Republic of 
      Korea.
FAU - Jo, Woori
AU  - Jo W
AD  - Asan Institute for Life Sciences, Asan Medical Center, Seoul 138-736, Republic of 
      Korea.
FAU - Choi, Hyun-Ji
AU  - Choi HJ
AD  - Asan Institute for Life Sciences, Asan Medical Center, Seoul 138-736, Republic of 
      Korea.
FAU - Jang, Sungwoong
AU  - Jang S
AD  - Asan Institute for Life Sciences, Asan Medical Center, Seoul 138-736, Republic of 
      Korea.
FAU - Lee, Hyo-Ju
AU  - Lee HJ
AD  - Asan Institute for Life Sciences, Asan Medical Center, Seoul 138-736, Republic of 
      Korea.
FAU - Woo, Dong-Cheul
AU  - Woo DC
AD  - Asan Institute for Life Sciences, Asan Medical Center, Seoul 138-736, Republic of 
      Korea.
FAU - Kim, Jeong Kon
AU  - Kim JK
AD  - Department of Radiology, University of Ulsan College of Medicine, Asan Medical 
      Center, Seoul 138-736, Republic of Korea.
FAU - Kim, Kyung Won
AU  - Kim KW
AD  - Department of Radiology, University of Ulsan College of Medicine, Asan Medical 
      Center, Seoul 138-736, Republic of Korea.
FAU - Yu, Eun Sil
AU  - Yu ES
AD  - Department of Pathology, University of Ulsan College of Medicine, Asan Medical 
      Center, Seoul 138-736, Republic of Korea.
FAU - Son, Woo-Chan
AU  - Son WC
AD  - Asan Institute for Life Sciences, Asan Medical Center, Seoul 138-736, Republic of 
      Korea; Department of Pathology, University of Ulsan College of Medicine, Asan 
      Medical Center, Seoul 138-736, Republic of Korea. wcson@amc.seoul.kr.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - United States
TA  - Comp Med
JT  - Comparative medicine
JID - 100900466
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Area Under Curve
MH  - Biopsy
MH  - Choline Deficiency/complications
MH  - Disease Models, Animal
MH  - Liver/*pathology
MH  - *Magnetic Resonance Imaging
MH  - Methionine/deficiency
MH  - Mice, Inbred C57BL
MH  - Non-alcoholic Fatty Liver Disease/etiology/*pathology
MH  - Predictive Value of Tests
MH  - ROC Curve
MH  - Reproducibility of Results
MH  - Severity of Illness Index
PMC - PMC4617331
EDAT- 2015/10/17 06:00
MHDA- 2016/08/04 06:00
PMCR- 2016/04/01
CRDT- 2015/10/17 06:00
PHST- 2015/10/17 06:00 [entrez]
PHST- 2015/10/17 06:00 [pubmed]
PHST- 2016/08/04 06:00 [medline]
PHST- 2016/04/01 00:00 [pmc-release]
AID - 2015000409 [pii]
PST - ppublish
SO  - Comp Med. 2015 Oct;65(5):409-15.