PMID- 26474053
OWN - NLM
STAT- MEDLINE
DCOM- 20160603
LR  - 20181113
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 10
DP  - 2015
TI  - Aging Impairs the Ability of Conventional Dendritic Cells to Cross-Prime CD8+ T 
      Cells upon Stimulation with a TLR7 Ligand.
PG  - e0140672
LID - 10.1371/journal.pone.0140672 [doi]
LID - e0140672
AB  - The aging process is accompanied by altered immune system functioning and an 
      increased risk of infection. Dendritic cells (DCs) are antigen-presenting cells 
      that play a key role in both adaptive and innate immunity, but how aging affects 
      DCs and their influence on immunity has not been thoroughly established. Here we 
      examined the function of conventional DCs (cDCs) in old mice after TLR7 
      stimulation, focusing on their ability to cross-prime CD8+ T cells. Using polyU, 
      a synthetic ssRNA analog, as TLR7 ligand and OVA as an antigen (Ag) model, we 
      found that cDCs from old mice have a poor ability to stimulate a CD8+ T 
      cell-mediated cytotoxic response. cDCs from old mice exhibit alterations in 
      Ag-processing machinery and TLR7 activation. Remarkably, CD8alpha+ cDCs from old mice 
      have an impaired ability to activate naive CD8+ T cells and, moreover, a lower 
      capacity to mature and to process exogenous Ag. Taken together, our results 
      suggest that immunosenescence impacts cDC function, affecting the activation of 
      naive CD8+ T cells and the generation of effector cytotoxic T cells.
FAU - Zacca, Estefania R
AU  - Zacca ER
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
FAU - Crespo, Maria I
AU  - Crespo MI
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
FAU - Acland, Rachel P
AU  - Acland RP
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
FAU - Roselli, Emiliano
AU  - Roselli E
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
FAU - Nunez, Nicolas G
AU  - Nunez NG
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
FAU - Maccioni, Mariana
AU  - Maccioni M
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
FAU - Maletto, Belkys A
AU  - Maletto BA
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
FAU - Pistoresi-Palencia, Maria C
AU  - Pistoresi-Palencia MC
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
FAU - Moron, Gabriel
AU  - Moron G
AD  - Centro de Investigaciones en Bioquimica Clinica e Inmunologia, Consejo Nacional 
      de Investigaciones Cientificas y Tecnicas, Facultad de Ciencias Quimicas, 
      Universidad Nacional de Cordoba, Cordoba, Argentina.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151016
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antigens)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Tlr7 protein, mouse)
RN  - 0 (Toll-Like Receptor 7)
RN  - 27416-86-0 (Poly U)
SB  - IM
MH  - Aging/*immunology
MH  - Animals
MH  - *Antigen Presentation
MH  - Antigens/immunology/pharmacology
MH  - CD8-Positive T-Lymphocytes/*enzymology
MH  - Cross-Priming/drug effects/*immunology
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - *Immunity, Cellular
MH  - Membrane Glycoproteins/*immunology
MH  - Mice
MH  - Poly U/immunology/pharmacology
MH  - Toll-Like Receptor 7/*immunology
PMC - PMC4608578
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/10/17 06:00
MHDA- 2016/06/04 06:00
PMCR- 2015/10/16
CRDT- 2015/10/17 06:00
PHST- 2015/07/21 00:00 [received]
PHST- 2015/09/29 00:00 [accepted]
PHST- 2015/10/17 06:00 [entrez]
PHST- 2015/10/17 06:00 [pubmed]
PHST- 2016/06/04 06:00 [medline]
PHST- 2015/10/16 00:00 [pmc-release]
AID - PONE-D-15-32037 [pii]
AID - 10.1371/journal.pone.0140672 [doi]
PST - epublish
SO  - PLoS One. 2015 Oct 16;10(10):e0140672. doi: 10.1371/journal.pone.0140672. 
      eCollection 2015.